Phosphotransbutyrylase expression in Bacillus megaterium

The molecular analysis of a genomic region of B. megaterium revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). The enzyme activity was measured throughout the different phases of growth in B. megaterium, and its activity was found to be maximal in the late exponentia...

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Detalles Bibliográficos
Autores principales: Vazquez, G.J., Pettinari, M.J., Méndez, B.S.
Formato: JOUR
Materias:
bacterial enzyme
isoleucine
phosphotransbutyrylase
unclassified drug
valine
article
Bacillus megaterium
bacterial growth
enzyme activity
enzyme substrate
gene expression regulation
nonhuman
nucleotide sequence
priority journal
protein expression
regulatory mechanism
Amino Acids, Branched-Chain
Culture Media
DNA-Binding Proteins
DNA-Directed RNA Polymerases
Gene Expression Regulation, Bacterial
Kinetics
Phosphate Acetyltransferase
RNA Polymerase Sigma 54
Sigma Factor
Bacteria (microorganisms)
Posibacteria
Prokaryota
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03438651_v42_n5_p345_Vazquez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The molecular analysis of a genomic region of B. megaterium revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). The enzyme activity was measured throughout the different phases of growth in B. megaterium, and its activity was found to be maximal in the late exponential growth phase. The branched amino acids isoleucine and valine activated Ptb expression. PtbBm was capable of using butyryl-CoA and 2-methyl-propionyl CoA as substrates. ActBm, a σ54 regulator from B. megaterium whose gene is situated upstream from the ptb gene, activated its expression.

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