Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment
A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported...
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todo:paper_03438651_v59_n5_p514_Lopez2023-10-03T15:26:16Z Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment López, N.I. Pettinari, M.J. Stackebrandt, E. Tribelli, P.M. Põtter, M. Steinbüchel, A. Méndez, B.S. arginine deiminase beta galactosidase casein esculin gelatin indole poly(3 hydroxybutyric acid) RNA 16S urease acidification Antarctica article bacterial growth bacterial strain controlled study denitrification DNA DNA hybridization gene sequence hydrolysis nitrate reducing bacterium nonhuman nucleotide sequence priority journal Pseudomonas pseudomonas extremaustralis Pseudomonas sensu stricto temperature sensitivity Antarctic Regions DNA, Bacterial DNA, Ribosomal Hydroxybutyrates Molecular Sequence Data Phylogeny Polyesters Pseudomonas RNA, Ribosomal, 16S Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas veronii A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogenetic affiliation. Strain 14-3T is closely related to Pseudomonas veronii DSM 11331T, sharing 99.7% sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (35.1%). Tests for arginine dihydrolase and nitrate reduction were positive, while those for denitrification, indol production, glucose acidification, urease, -galactosidase, esculin, caseine and gelatin hydrolysis were negative. Growth of this bacterium occurred in a range from 4 to 37°C but not at 42°C. It accumulated poly(3-hydroxybutyrate) when grown on sodium octanoate medium. Strain 14-3T therefore represents the type strain of a new species, for which the name Pseudomonas extremaustralis sp. nov. is proposed. The type strain 14-3T has been deposited as DSM 17835T and as CIP 109839T. © 2009 Springer Science+Business Media, LLC. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03438651_v59_n5_p514_Lopez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
arginine deiminase beta galactosidase casein esculin gelatin indole poly(3 hydroxybutyric acid) RNA 16S urease acidification Antarctica article bacterial growth bacterial strain controlled study denitrification DNA DNA hybridization gene sequence hydrolysis nitrate reducing bacterium nonhuman nucleotide sequence priority journal Pseudomonas pseudomonas extremaustralis Pseudomonas sensu stricto temperature sensitivity Antarctic Regions DNA, Bacterial DNA, Ribosomal Hydroxybutyrates Molecular Sequence Data Phylogeny Polyesters Pseudomonas RNA, Ribosomal, 16S Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas veronii |
| spellingShingle |
arginine deiminase beta galactosidase casein esculin gelatin indole poly(3 hydroxybutyric acid) RNA 16S urease acidification Antarctica article bacterial growth bacterial strain controlled study denitrification DNA DNA hybridization gene sequence hydrolysis nitrate reducing bacterium nonhuman nucleotide sequence priority journal Pseudomonas pseudomonas extremaustralis Pseudomonas sensu stricto temperature sensitivity Antarctic Regions DNA, Bacterial DNA, Ribosomal Hydroxybutyrates Molecular Sequence Data Phylogeny Polyesters Pseudomonas RNA, Ribosomal, 16S Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas veronii López, N.I. Pettinari, M.J. Stackebrandt, E. Tribelli, P.M. Põtter, M. Steinbüchel, A. Méndez, B.S. Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment |
| topic_facet |
arginine deiminase beta galactosidase casein esculin gelatin indole poly(3 hydroxybutyric acid) RNA 16S urease acidification Antarctica article bacterial growth bacterial strain controlled study denitrification DNA DNA hybridization gene sequence hydrolysis nitrate reducing bacterium nonhuman nucleotide sequence priority journal Pseudomonas pseudomonas extremaustralis Pseudomonas sensu stricto temperature sensitivity Antarctic Regions DNA, Bacterial DNA, Ribosomal Hydroxybutyrates Molecular Sequence Data Phylogeny Polyesters Pseudomonas RNA, Ribosomal, 16S Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas veronii |
| description |
A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogenetic affiliation. Strain 14-3T is closely related to Pseudomonas veronii DSM 11331T, sharing 99.7% sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (35.1%). Tests for arginine dihydrolase and nitrate reduction were positive, while those for denitrification, indol production, glucose acidification, urease, -galactosidase, esculin, caseine and gelatin hydrolysis were negative. Growth of this bacterium occurred in a range from 4 to 37°C but not at 42°C. It accumulated poly(3-hydroxybutyrate) when grown on sodium octanoate medium. Strain 14-3T therefore represents the type strain of a new species, for which the name Pseudomonas extremaustralis sp. nov. is proposed. The type strain 14-3T has been deposited as DSM 17835T and as CIP 109839T. © 2009 Springer Science+Business Media, LLC. |
| format |
JOUR |
| author |
López, N.I. Pettinari, M.J. Stackebrandt, E. Tribelli, P.M. Põtter, M. Steinbüchel, A. Méndez, B.S. |
| author_facet |
López, N.I. Pettinari, M.J. Stackebrandt, E. Tribelli, P.M. Põtter, M. Steinbüchel, A. Méndez, B.S. |
| author_sort |
López, N.I. |
| title |
Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment |
| title_short |
Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment |
| title_full |
Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment |
| title_fullStr |
Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment |
| title_full_unstemmed |
Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment |
| title_sort |
pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment |
| url |
http://hdl.handle.net/20.500.12110/paper_03438651_v59_n5_p514_Lopez |
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