An extracellular acid polysaccharide from coelastrum sphaericum

An extracellular acid polysaccharide was precipitated at 70% ethanol, together with a mannose-containing carbohydrate, sucrose and glucose, from the culture media of Coelastrum sphaericum. Purification by repeated centrifugation and dialysis and gel permeation cromatography yielded a product compose...

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Autores principales: Rodríguez, M.C., Cerezo, A.S.
Formato: JOUR
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03650375_v87_n5-6_p197_Rodriguez
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03650375_v87_n5-6_p197_Rodriguez
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spelling todo:paper_03650375_v87_n5-6_p197_Rodriguez2023-10-03T15:27:58Z An extracellular acid polysaccharide from coelastrum sphaericum Rodríguez, M.C. Cerezo, A.S. An extracellular acid polysaccharide was precipitated at 70% ethanol, together with a mannose-containing carbohydrate, sucrose and glucose, from the culture media of Coelastrum sphaericum. Purification by repeated centrifugation and dialysis and gel permeation cromatography yielded a product composed by an acid polysaccharide together with lesser amounts of a mannose-containing carbohydrate. Methylation analysis showed that the acid polysaccharide had a linear backbone with an average structural unit formed by 3-linked L-rhamnopyranose (2 moles), 3-linked D-galactopyranose (1 mole), 3-linked D-glucopyranose (1 mole) and 4-linked D-glucopyranuronic acid (1 mole). The chain had L-rhamnopyranose (1.9 mol%) and D-galactofuranose (0.5 mol %) single stubs 4-linked to the rhamnopyranose units in the backbone. Fil:Rodríguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03650375_v87_n5-6_p197_Rodriguez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description An extracellular acid polysaccharide was precipitated at 70% ethanol, together with a mannose-containing carbohydrate, sucrose and glucose, from the culture media of Coelastrum sphaericum. Purification by repeated centrifugation and dialysis and gel permeation cromatography yielded a product composed by an acid polysaccharide together with lesser amounts of a mannose-containing carbohydrate. Methylation analysis showed that the acid polysaccharide had a linear backbone with an average structural unit formed by 3-linked L-rhamnopyranose (2 moles), 3-linked D-galactopyranose (1 mole), 3-linked D-glucopyranose (1 mole) and 4-linked D-glucopyranuronic acid (1 mole). The chain had L-rhamnopyranose (1.9 mol%) and D-galactofuranose (0.5 mol %) single stubs 4-linked to the rhamnopyranose units in the backbone.
format JOUR
author Rodríguez, M.C.
Cerezo, A.S.
spellingShingle Rodríguez, M.C.
Cerezo, A.S.
An extracellular acid polysaccharide from coelastrum sphaericum
author_facet Rodríguez, M.C.
Cerezo, A.S.
author_sort Rodríguez, M.C.
title An extracellular acid polysaccharide from coelastrum sphaericum
title_short An extracellular acid polysaccharide from coelastrum sphaericum
title_full An extracellular acid polysaccharide from coelastrum sphaericum
title_fullStr An extracellular acid polysaccharide from coelastrum sphaericum
title_full_unstemmed An extracellular acid polysaccharide from coelastrum sphaericum
title_sort extracellular acid polysaccharide from coelastrum sphaericum
url http://hdl.handle.net/20.500.12110/paper_03650375_v87_n5-6_p197_Rodriguez
work_keys_str_mv AT rodriguezmc anextracellularacidpolysaccharidefromcoelastrumsphaericum
AT cerezoas anextracellularacidpolysaccharidefromcoelastrumsphaericum
AT rodriguezmc extracellularacidpolysaccharidefromcoelastrumsphaericum
AT cerezoas extracellularacidpolysaccharidefromcoelastrumsphaericum
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