A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli
Plasmid pPG1 from Staphylococcus aureus coding for ampicillin (Apr), gentamicin (Gmr) and amikacin (Akr) resistance was transformed into Escherichia coli. Transformation efficiency was about 2 × 103 transformants/μg of plasmid DNA. The plasmids present in the E. coli transformants were identical to...
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todo:paper_03781097_v80_n2-3_p147_Gadaleta2023-10-03T15:31:46Z A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli Gadaleta, P. Kaufman, S. Martini, P. Zorzópulos, J. Antibiotic resistance Hetero specific gene exchange Staphylococcus plasmid amikacin gentamicin amikacin ampicillin chloramphenicol gentamicin tetracycline antibiotic resistance article bacterium transformation Escherichia coli genetics penicillin resistance plasmid Staphylococcus aureus dna hybridization escherichia coli nonhuman plasmid priority journal southern blotting staphylococcus Amikacin Ampicillin Resistance Drug Resistance, Microbial Escherichia coli Gentamicins Plasmids Staphylococcus aureus Support, Non-U.S. Gov't Transformation, Bacterial Escherichia coli Staphylococcus Staphylococcus aureus Plasmid pPG1 from Staphylococcus aureus coding for ampicillin (Apr), gentamicin (Gmr) and amikacin (Akr) resistance was transformed into Escherichia coli. Transformation efficiency was about 2 × 103 transformants/μg of plasmid DNA. The plasmids present in the E. coli transformants were identical to pPG1 according to their restriction patterns. The copy number of pPG1 was estimated to be at least 20-times less in E. coli than in S. aureus. The minimal inhibitory concentrations (MICs) for Ap and Gm were lower in E. coli than in S. aureus. However, the MIC for Ak was higher in E. coli transformants than in S. aureus. pPG1 was maintained in the E. coli transformants for at least 80 generations at 37°C without antibiotic selection pressure. © 1991. Fil:Gadaleta, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v80_n2-3_p147_Gadaleta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antibiotic resistance Hetero specific gene exchange Staphylococcus plasmid amikacin gentamicin amikacin ampicillin chloramphenicol gentamicin tetracycline antibiotic resistance article bacterium transformation Escherichia coli genetics penicillin resistance plasmid Staphylococcus aureus dna hybridization escherichia coli nonhuman plasmid priority journal southern blotting staphylococcus Amikacin Ampicillin Resistance Drug Resistance, Microbial Escherichia coli Gentamicins Plasmids Staphylococcus aureus Support, Non-U.S. Gov't Transformation, Bacterial Escherichia coli Staphylococcus Staphylococcus aureus |
| spellingShingle |
Antibiotic resistance Hetero specific gene exchange Staphylococcus plasmid amikacin gentamicin amikacin ampicillin chloramphenicol gentamicin tetracycline antibiotic resistance article bacterium transformation Escherichia coli genetics penicillin resistance plasmid Staphylococcus aureus dna hybridization escherichia coli nonhuman plasmid priority journal southern blotting staphylococcus Amikacin Ampicillin Resistance Drug Resistance, Microbial Escherichia coli Gentamicins Plasmids Staphylococcus aureus Support, Non-U.S. Gov't Transformation, Bacterial Escherichia coli Staphylococcus Staphylococcus aureus Gadaleta, P. Kaufman, S. Martini, P. Zorzópulos, J. A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli |
| topic_facet |
Antibiotic resistance Hetero specific gene exchange Staphylococcus plasmid amikacin gentamicin amikacin ampicillin chloramphenicol gentamicin tetracycline antibiotic resistance article bacterium transformation Escherichia coli genetics penicillin resistance plasmid Staphylococcus aureus dna hybridization escherichia coli nonhuman plasmid priority journal southern blotting staphylococcus Amikacin Ampicillin Resistance Drug Resistance, Microbial Escherichia coli Gentamicins Plasmids Staphylococcus aureus Support, Non-U.S. Gov't Transformation, Bacterial Escherichia coli Staphylococcus Staphylococcus aureus |
| description |
Plasmid pPG1 from Staphylococcus aureus coding for ampicillin (Apr), gentamicin (Gmr) and amikacin (Akr) resistance was transformed into Escherichia coli. Transformation efficiency was about 2 × 103 transformants/μg of plasmid DNA. The plasmids present in the E. coli transformants were identical to pPG1 according to their restriction patterns. The copy number of pPG1 was estimated to be at least 20-times less in E. coli than in S. aureus. The minimal inhibitory concentrations (MICs) for Ap and Gm were lower in E. coli than in S. aureus. However, the MIC for Ak was higher in E. coli transformants than in S. aureus. pPG1 was maintained in the E. coli transformants for at least 80 generations at 37°C without antibiotic selection pressure. © 1991. |
| format |
JOUR |
| author |
Gadaleta, P. Kaufman, S. Martini, P. Zorzópulos, J. |
| author_facet |
Gadaleta, P. Kaufman, S. Martini, P. Zorzópulos, J. |
| author_sort |
Gadaleta, P. |
| title |
A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli |
| title_short |
A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli |
| title_full |
A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli |
| title_fullStr |
A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli |
| title_full_unstemmed |
A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli |
| title_sort |
staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in escherichia coli |
| url |
http://hdl.handle.net/20.500.12110/paper_03781097_v80_n2-3_p147_Gadaleta |
| work_keys_str_mv |
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1807321293144457216 |