Morphine inhibits an α9-acetylcholine nicotinic receptor-mediated response by a mechanism which does not involve opioid receptors
Nicotinic acetylcholine (nACh) receptors are known to be targets for modulation by a number of substances, including the opiates. It is known that acetylcholine (ACh) coexists with opioid peptides in cochlear efferent neurons, and such a colocalization has been proposed for the vestibular system. In...
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todo:paper_03785955_v149_n1-2_p167_Lioudyno2023-10-03T15:33:09Z Morphine inhibits an α9-acetylcholine nicotinic receptor-mediated response by a mechanism which does not involve opioid receptors Lioudyno, M.I. Verbitsky, M. Holt, J.C. Elgoyhen, A.B. Guth, P.S. Efferent Hair cell Interaction Morphine nACh receptor Vestibular system morphine nicotinic receptor opiate receptor acetylcholine Chrna9 protein, mouse morphine naloxone narcotic agent narcotic antagonist nicotinic receptor animal cell animal experiment article controlled study efferent nerve frog hair cell nonhuman patch clamp priority journal receptor blocking vestibular nervous system Xenopus laevis animal drug effect electric conductivity hair cell metabolism oocyte physiology Acetylcholine Animals Electric Conductivity Hair Cells Morphine Naloxone Narcotic Antagonists Narcotics Oocytes Rana pipiens Receptors, Nicotinic Xenopus laevis Nicotinic acetylcholine (nACh) receptors are known to be targets for modulation by a number of substances, including the opiates. It is known that acetylcholine (ACh) coexists with opioid peptides in cochlear efferent neurons, and such a colocalization has been proposed for the vestibular system. In the present study we test the hypothesis that morphine, an opioid receptor agonist with a broad spectrum of selectivity, modulates α9nACh receptor-mediated responses in frog vestibular hair cells. Morphine dose-dependently and reversibly inhibited ACh-induced currents as recorded by the perforated patch-clamp method. In the presence of morphine the ACh dose-response curve was shifted to the right in a parallel fashion, suggesting a competitive interaction. However, naloxone did not antagonize the inhibition produced by morphine. To test the hypothesis that morphine could interact with the α9nACh receptor without the involvement of opioid receptors, experiments were performed using Xenopus laevis oocytes injected with the α9nACh receptor cRNA. The currents activated by ACh in Xenopus oocytes, a system that lacks opioid receptors, were also dose-dependently inhibited by morphine. We conclude that morphine inhibits the α9nACh receptor-mediated response in hair cells and Xenopus oocytes through a mechanism which does not involve opioid receptors but may be a direct block of the α9nACh receptor. Copyright (C) 2000 Elsevier Science B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03785955_v149_n1-2_p167_Lioudyno |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Efferent Hair cell Interaction Morphine nACh receptor Vestibular system morphine nicotinic receptor opiate receptor acetylcholine Chrna9 protein, mouse morphine naloxone narcotic agent narcotic antagonist nicotinic receptor animal cell animal experiment article controlled study efferent nerve frog hair cell nonhuman patch clamp priority journal receptor blocking vestibular nervous system Xenopus laevis animal drug effect electric conductivity hair cell metabolism oocyte physiology Acetylcholine Animals Electric Conductivity Hair Cells Morphine Naloxone Narcotic Antagonists Narcotics Oocytes Rana pipiens Receptors, Nicotinic Xenopus laevis |
| spellingShingle |
Efferent Hair cell Interaction Morphine nACh receptor Vestibular system morphine nicotinic receptor opiate receptor acetylcholine Chrna9 protein, mouse morphine naloxone narcotic agent narcotic antagonist nicotinic receptor animal cell animal experiment article controlled study efferent nerve frog hair cell nonhuman patch clamp priority journal receptor blocking vestibular nervous system Xenopus laevis animal drug effect electric conductivity hair cell metabolism oocyte physiology Acetylcholine Animals Electric Conductivity Hair Cells Morphine Naloxone Narcotic Antagonists Narcotics Oocytes Rana pipiens Receptors, Nicotinic Xenopus laevis Lioudyno, M.I. Verbitsky, M. Holt, J.C. Elgoyhen, A.B. Guth, P.S. Morphine inhibits an α9-acetylcholine nicotinic receptor-mediated response by a mechanism which does not involve opioid receptors |
| topic_facet |
Efferent Hair cell Interaction Morphine nACh receptor Vestibular system morphine nicotinic receptor opiate receptor acetylcholine Chrna9 protein, mouse morphine naloxone narcotic agent narcotic antagonist nicotinic receptor animal cell animal experiment article controlled study efferent nerve frog hair cell nonhuman patch clamp priority journal receptor blocking vestibular nervous system Xenopus laevis animal drug effect electric conductivity hair cell metabolism oocyte physiology Acetylcholine Animals Electric Conductivity Hair Cells Morphine Naloxone Narcotic Antagonists Narcotics Oocytes Rana pipiens Receptors, Nicotinic Xenopus laevis |
| description |
Nicotinic acetylcholine (nACh) receptors are known to be targets for modulation by a number of substances, including the opiates. It is known that acetylcholine (ACh) coexists with opioid peptides in cochlear efferent neurons, and such a colocalization has been proposed for the vestibular system. In the present study we test the hypothesis that morphine, an opioid receptor agonist with a broad spectrum of selectivity, modulates α9nACh receptor-mediated responses in frog vestibular hair cells. Morphine dose-dependently and reversibly inhibited ACh-induced currents as recorded by the perforated patch-clamp method. In the presence of morphine the ACh dose-response curve was shifted to the right in a parallel fashion, suggesting a competitive interaction. However, naloxone did not antagonize the inhibition produced by morphine. To test the hypothesis that morphine could interact with the α9nACh receptor without the involvement of opioid receptors, experiments were performed using Xenopus laevis oocytes injected with the α9nACh receptor cRNA. The currents activated by ACh in Xenopus oocytes, a system that lacks opioid receptors, were also dose-dependently inhibited by morphine. We conclude that morphine inhibits the α9nACh receptor-mediated response in hair cells and Xenopus oocytes through a mechanism which does not involve opioid receptors but may be a direct block of the α9nACh receptor. Copyright (C) 2000 Elsevier Science B.V. |
| format |
JOUR |
| author |
Lioudyno, M.I. Verbitsky, M. Holt, J.C. Elgoyhen, A.B. Guth, P.S. |
| author_facet |
Lioudyno, M.I. Verbitsky, M. Holt, J.C. Elgoyhen, A.B. Guth, P.S. |
| author_sort |
Lioudyno, M.I. |
| title |
Morphine inhibits an α9-acetylcholine nicotinic receptor-mediated response by a mechanism which does not involve opioid receptors |
| title_short |
Morphine inhibits an α9-acetylcholine nicotinic receptor-mediated response by a mechanism which does not involve opioid receptors |
| title_full |
Morphine inhibits an α9-acetylcholine nicotinic receptor-mediated response by a mechanism which does not involve opioid receptors |
| title_fullStr |
Morphine inhibits an α9-acetylcholine nicotinic receptor-mediated response by a mechanism which does not involve opioid receptors |
| title_full_unstemmed |
Morphine inhibits an α9-acetylcholine nicotinic receptor-mediated response by a mechanism which does not involve opioid receptors |
| title_sort |
morphine inhibits an α9-acetylcholine nicotinic receptor-mediated response by a mechanism which does not involve opioid receptors |
| url |
http://hdl.handle.net/20.500.12110/paper_03785955_v149_n1-2_p167_Lioudyno |
| work_keys_str_mv |
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