Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters

Gonadotropin-releasing hormone (GnRH) is the hypothalamic hormone that regulates the reproductive system by stimulating release of gonadotropins from the anterior pituitary gland. The molecular variants of the reproductive neuropeptide GnRH were characterized from brain tissue of three perciform spe...

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Autores principales: Miranda, L.A., Montaner, A.D., Ansaldo, M., Affanni, J.M., Somoza, G.M.
Formato: JOUR
Materias:
GnRH fish
Perciformes
Reproduction
Reverse phase HPLC Radioimmunoassay
Chaenocephalus aceratus
chromatography
gonadorelin
high performance liquid chromatography
Notothenia rossi
protein analysis
Pseudochaenichthys georgianus
radioimmunoassay
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_07224060_v21_n2_p122_Miranda
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_07224060_v21_n2_p122_Miranda
record_format dspace
spelling todo:paper_07224060_v21_n2_p122_Miranda2023-10-03T15:36:55Z Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters Miranda, L.A. Montaner, A.D. Ansaldo, M. Affanni, J.M. Somoza, G.M. GnRH fish Perciformes Reproduction Reverse phase HPLC Radioimmunoassay Chaenocephalus aceratus chromatography gonadorelin high performance liquid chromatography Notothenia rossi protein analysis Pseudochaenichthys georgianus radioimmunoassay Gonadotropin-releasing hormone (GnRH) is the hypothalamic hormone that regulates the reproductive system by stimulating release of gonadotropins from the anterior pituitary gland. The molecular variants of the reproductive neuropeptide GnRH were characterized from brain tissue of three perciform species from Antarctic waters: Pseudochaenichthys georgianus, Chaenocephalus aceratus, and Notothenia rossi. The study involved reverse phase high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassay (RIA) with two antisera that recognize all GnRH variants already identified: PBL 45 and PBL 49. The results showed that brain extracts of P. georgianus, C. aceratus, and N: rossi contain, like those of other perciform fish, three forms of GnRH likely to be: sbGnRH (seabream GnRH), cGnRH-II (chicken GnRH II) and sGnRH (salmon GnRH). They also showed evidence for the presence of a fourth GnRH variant, chromatographically and immunologically different from the other known forms of the vertebrate hormone. Although final conclusions will require isolation, purification, and sequencing of these molecules, these results offer encouraging possibilities of further advances in the characterization of a multiplicity of GnRH molecular variants. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07224060_v21_n2_p122_Miranda
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic GnRH fish
Perciformes
Reproduction
Reverse phase HPLC Radioimmunoassay
Chaenocephalus aceratus
chromatography
gonadorelin
high performance liquid chromatography
Notothenia rossi
protein analysis
Pseudochaenichthys georgianus
radioimmunoassay
spellingShingle GnRH fish
Perciformes
Reproduction
Reverse phase HPLC Radioimmunoassay
Chaenocephalus aceratus
chromatography
gonadorelin
high performance liquid chromatography
Notothenia rossi
protein analysis
Pseudochaenichthys georgianus
radioimmunoassay
Miranda, L.A.
Montaner, A.D.
Ansaldo, M.
Affanni, J.M.
Somoza, G.M.
Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters
topic_facet GnRH fish
Perciformes
Reproduction
Reverse phase HPLC Radioimmunoassay
Chaenocephalus aceratus
chromatography
gonadorelin
high performance liquid chromatography
Notothenia rossi
protein analysis
Pseudochaenichthys georgianus
radioimmunoassay
description Gonadotropin-releasing hormone (GnRH) is the hypothalamic hormone that regulates the reproductive system by stimulating release of gonadotropins from the anterior pituitary gland. The molecular variants of the reproductive neuropeptide GnRH were characterized from brain tissue of three perciform species from Antarctic waters: Pseudochaenichthys georgianus, Chaenocephalus aceratus, and Notothenia rossi. The study involved reverse phase high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassay (RIA) with two antisera that recognize all GnRH variants already identified: PBL 45 and PBL 49. The results showed that brain extracts of P. georgianus, C. aceratus, and N: rossi contain, like those of other perciform fish, three forms of GnRH likely to be: sbGnRH (seabream GnRH), cGnRH-II (chicken GnRH II) and sGnRH (salmon GnRH). They also showed evidence for the presence of a fourth GnRH variant, chromatographically and immunologically different from the other known forms of the vertebrate hormone. Although final conclusions will require isolation, purification, and sequencing of these molecules, these results offer encouraging possibilities of further advances in the characterization of a multiplicity of GnRH molecular variants.
format JOUR
author Miranda, L.A.
Montaner, A.D.
Ansaldo, M.
Affanni, J.M.
Somoza, G.M.
author_facet Miranda, L.A.
Montaner, A.D.
Ansaldo, M.
Affanni, J.M.
Somoza, G.M.
author_sort Miranda, L.A.
title Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters
title_short Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters
title_full Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters
title_fullStr Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters
title_full_unstemmed Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters
title_sort characterization of brain gonadotropin-releasing hormone (gnrh) molecular variants in brain extracts from different perciform fishes from antarctic waters
url http://hdl.handle.net/20.500.12110/paper_07224060_v21_n2_p122_Miranda
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