Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae
Copper toxicity in different embryonic and larval stages of the common South American toad Rhinella arenarum was evaluated by means of continuous and 24-h pulse treatments in 12 different developmental stages. Lethal concentrations (LC) of 10, 50, and 90% of continuous treatment with Cu from early b...
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| Formato: | JOUR |
| Lenguaje: | English |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_07307268_v30_n12_p2771_Aronzon |
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todo:paper_07307268_v30_n12_p2771_Aronzon2023-10-03T15:37:20Z Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae Aronzon, C.M. Sandoval, M.T. Herkovits, J. Pérezcoll, C.S. Amphibian embryos AMPHITOX Copper Stage-dependent toxicity Teratogenesis Adverse effect Amphibian embryos AMPHITOX Body sizes Copper toxicity Developmental stage Evolutionary perspective Exposure-time Gastrula stage High resistance Hind limbs Larval periods Larval stage Lethal concentration Teratogenesis Toxicity Copper copper concentration (composition) copper embryo environmental protection frog ion larva toxicity agenesis animal embryo animal experiment article blastula body size developmental stage environmental protection evoked muscle response evolutionary developmental biology female gastrula gill larva LC 50 limb bud microcephaly mouth mouth malformation nonhuman organogenesis parietal operculum priority journal Rhinella arenarum South America tail teratogenesis toad Animals Bufo arenarum Copper Embryo, Nonmammalian Gills Larva Lethal Dose 50 Teratogens Water Pollutants, Chemical South America Amphibia Bufo americanus Copper toxicity in different embryonic and larval stages of the common South American toad Rhinella arenarum was evaluated by means of continuous and 24-h pulse treatments in 12 different developmental stages. Lethal concentrations (LC) of 10, 50, and 90% of continuous treatment with Cu from early blastula (S.4), complete operculum (S.25), and hind limb bud (S.28) stages were plotted from 24 to 168h, resulting from S.4 in a 24-h LC50 of 137μg Cu 2+/L and a 168-h LC50 of 19.5μg Cu 2+/L. This result was in agreement with pulse treatments that showed a high resistance to Cu at blastula and gastrula stages, whereas the organogenic period, between muscular response (S.18) and open mouth (S.21), was very susceptible to this metal. Continuous treatments from S.25 showed no significant differences along exposure time (168-h LC50=51μg Cu 2+/L), but in the case of S.28 toxicity increased slightly from a 24-h LC50 of 138.6μg Cu 2+/L to a 168-h LC50 of 104μg Cu 2+/L, pointing out that, although the larval period was significantly more resistant to Cu, there was also a remarkable stage-dependent susceptibility to this metal. Copper teratogenic potential was approximately two, and main adverse effects were reduced body size, axial flexure, microcephaly, acephaly, mouth malformations, agenesis of or underdeveloped gills, agenesis of or underdeveloped tail, and hydropsy. The results are discussed considering Cu toxicity mechanisms, an evolutionary perspective, and environmental protection. © 2011 SETAC. Fil:Aronzon, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07307268_v30_n12_p2771_Aronzon |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
English |
| orig_language_str_mv |
English |
| topic |
Amphibian embryos AMPHITOX Copper Stage-dependent toxicity Teratogenesis Adverse effect Amphibian embryos AMPHITOX Body sizes Copper toxicity Developmental stage Evolutionary perspective Exposure-time Gastrula stage High resistance Hind limbs Larval periods Larval stage Lethal concentration Teratogenesis Toxicity Copper copper concentration (composition) copper embryo environmental protection frog ion larva toxicity agenesis animal embryo animal experiment article blastula body size developmental stage environmental protection evoked muscle response evolutionary developmental biology female gastrula gill larva LC 50 limb bud microcephaly mouth mouth malformation nonhuman organogenesis parietal operculum priority journal Rhinella arenarum South America tail teratogenesis toad Animals Bufo arenarum Copper Embryo, Nonmammalian Gills Larva Lethal Dose 50 Teratogens Water Pollutants, Chemical South America Amphibia Bufo americanus |
| spellingShingle |
Amphibian embryos AMPHITOX Copper Stage-dependent toxicity Teratogenesis Adverse effect Amphibian embryos AMPHITOX Body sizes Copper toxicity Developmental stage Evolutionary perspective Exposure-time Gastrula stage High resistance Hind limbs Larval periods Larval stage Lethal concentration Teratogenesis Toxicity Copper copper concentration (composition) copper embryo environmental protection frog ion larva toxicity agenesis animal embryo animal experiment article blastula body size developmental stage environmental protection evoked muscle response evolutionary developmental biology female gastrula gill larva LC 50 limb bud microcephaly mouth mouth malformation nonhuman organogenesis parietal operculum priority journal Rhinella arenarum South America tail teratogenesis toad Animals Bufo arenarum Copper Embryo, Nonmammalian Gills Larva Lethal Dose 50 Teratogens Water Pollutants, Chemical South America Amphibia Bufo americanus Aronzon, C.M. Sandoval, M.T. Herkovits, J. Pérezcoll, C.S. Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae |
| topic_facet |
Amphibian embryos AMPHITOX Copper Stage-dependent toxicity Teratogenesis Adverse effect Amphibian embryos AMPHITOX Body sizes Copper toxicity Developmental stage Evolutionary perspective Exposure-time Gastrula stage High resistance Hind limbs Larval periods Larval stage Lethal concentration Teratogenesis Toxicity Copper copper concentration (composition) copper embryo environmental protection frog ion larva toxicity agenesis animal embryo animal experiment article blastula body size developmental stage environmental protection evoked muscle response evolutionary developmental biology female gastrula gill larva LC 50 limb bud microcephaly mouth mouth malformation nonhuman organogenesis parietal operculum priority journal Rhinella arenarum South America tail teratogenesis toad Animals Bufo arenarum Copper Embryo, Nonmammalian Gills Larva Lethal Dose 50 Teratogens Water Pollutants, Chemical South America Amphibia Bufo americanus |
| description |
Copper toxicity in different embryonic and larval stages of the common South American toad Rhinella arenarum was evaluated by means of continuous and 24-h pulse treatments in 12 different developmental stages. Lethal concentrations (LC) of 10, 50, and 90% of continuous treatment with Cu from early blastula (S.4), complete operculum (S.25), and hind limb bud (S.28) stages were plotted from 24 to 168h, resulting from S.4 in a 24-h LC50 of 137μg Cu 2+/L and a 168-h LC50 of 19.5μg Cu 2+/L. This result was in agreement with pulse treatments that showed a high resistance to Cu at blastula and gastrula stages, whereas the organogenic period, between muscular response (S.18) and open mouth (S.21), was very susceptible to this metal. Continuous treatments from S.25 showed no significant differences along exposure time (168-h LC50=51μg Cu 2+/L), but in the case of S.28 toxicity increased slightly from a 24-h LC50 of 138.6μg Cu 2+/L to a 168-h LC50 of 104μg Cu 2+/L, pointing out that, although the larval period was significantly more resistant to Cu, there was also a remarkable stage-dependent susceptibility to this metal. Copper teratogenic potential was approximately two, and main adverse effects were reduced body size, axial flexure, microcephaly, acephaly, mouth malformations, agenesis of or underdeveloped gills, agenesis of or underdeveloped tail, and hydropsy. The results are discussed considering Cu toxicity mechanisms, an evolutionary perspective, and environmental protection. © 2011 SETAC. |
| format |
JOUR |
| author |
Aronzon, C.M. Sandoval, M.T. Herkovits, J. Pérezcoll, C.S. |
| author_facet |
Aronzon, C.M. Sandoval, M.T. Herkovits, J. Pérezcoll, C.S. |
| author_sort |
Aronzon, C.M. |
| title |
Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae |
| title_short |
Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae |
| title_full |
Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae |
| title_fullStr |
Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae |
| title_full_unstemmed |
Stage-dependent susceptibility to copper in Rhinella arenarum embryos and larvae |
| title_sort |
stage-dependent susceptibility to copper in rhinella arenarum embryos and larvae |
| url |
http://hdl.handle.net/20.500.12110/paper_07307268_v30_n12_p2771_Aronzon |
| work_keys_str_mv |
AT aronzoncm stagedependentsusceptibilitytocopperinrhinellaarenarumembryosandlarvae AT sandovalmt stagedependentsusceptibilitytocopperinrhinellaarenarumembryosandlarvae AT herkovitsj stagedependentsusceptibilitytocopperinrhinellaarenarumembryosandlarvae AT perezcollcs stagedependentsusceptibilitytocopperinrhinellaarenarumembryosandlarvae |
| _version_ |
1807324363507105792 |