Intracellular melatonin distribution in cultured cell lines

A specific antibody combined with a fluorescein-labeled immunoglobulin was used to investigate the topographic distribution of melatonin in a variety of cells of different origins. Positive identification of both nuclear and cytosolic melatonin was confirmed in all the tested cells: Swiss 3T3 mouse...

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Autores principales:	Finocchiaro, L.M.E., Glikin, G.C.
Formato:	JOUR
Materias:	3T3 mouse fibroblasts Actin Bromodeoxyuridine Immunofluorescence Melatonin Serotonin actin broxuridine melatonin serotonin tubulin animal cell article cattle cell cycle cell strain 3t3 cellular distribution controlled study dna synthesis dog granulosa cell hela cell human human cell kidney cell monocyte mouse neuroblastoma cell nonhuman rat spleen cell teratocarcinoma Animals Cattle Cell Culture Techniques Cell Cycle Cell Line Cell Nucleus Cytoskeletal Proteins Cytosol DNA DNA Replication Dogs Female Fluorescent Antibody Technique, Indirect Humans Mice Rats Subcellular Fractions
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_07423098_v24_n1_p22_Finocchiaro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_07423098_v24_n1_p22_Finocchiaro
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spelling	todo:paper_07423098_v24_n1_p22_Finocchiaro2023-10-03T15:38:23Z Intracellular melatonin distribution in cultured cell lines Finocchiaro, L.M.E. Glikin, G.C. 3T3 mouse fibroblasts Actin Bromodeoxyuridine Immunofluorescence Melatonin Serotonin actin broxuridine melatonin serotonin tubulin animal cell article cattle cell cycle cell strain 3t3 cellular distribution controlled study dna synthesis dog granulosa cell hela cell human human cell kidney cell monocyte mouse neuroblastoma cell nonhuman rat spleen cell teratocarcinoma Animals Bromodeoxyuridine Cattle Cell Culture Techniques Cell Cycle Cell Line Cell Nucleus Cytoskeletal Proteins Cytosol DNA DNA Replication Dogs Female Fluorescent Antibody Technique, Indirect Humans Melatonin Mice Rats Subcellular Fractions A specific antibody combined with a fluorescein-labeled immunoglobulin was used to investigate the topographic distribution of melatonin in a variety of cells of different origins. Positive identification of both nuclear and cytosolic melatonin was confirmed in all the tested cells: Swiss 3T3 mouse fibroblasts, BCG1 bovine granulosa, NB41A3 mouse neuroblastoma, F9 mouse teratocarcinoma, MDCK normal canine kidney derived and human HeLa cell lines, as well as in human peripheral blood mononuclear leukocytes and rat splenic cells. In 3T3 mouse fibroblasts melatonin immunofluorescence partially colocalized with actin and serotonin immunostaining, but not with tubulin or actin stress fibers. Several distinct patterns of subcellular melatonin distribution, different from the bromodeoxyuridine-labeled replication profiles, have been discerned throughout the cell cycle of synchronized 3T3 cells. In addition, synchronized 3T3 mouse fibroblasts cultured in the presence of 10-3 M melatonin progressed more slowly through the cell cycle than control cells. These results suggest that melatonin may interact directly with nuclear and cytoskeletal structures probably affecting different cell functions such as cell cycle control, subcellular organization, and genome stability. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07423098_v24_n1_p22_Finocchiaro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	3T3 mouse fibroblasts Actin Bromodeoxyuridine Immunofluorescence Melatonin Serotonin actin broxuridine melatonin serotonin tubulin animal cell article cattle cell cycle cell strain 3t3 cellular distribution controlled study dna synthesis dog granulosa cell hela cell human human cell kidney cell monocyte mouse neuroblastoma cell nonhuman rat spleen cell teratocarcinoma Animals Bromodeoxyuridine Cattle Cell Culture Techniques Cell Cycle Cell Line Cell Nucleus Cytoskeletal Proteins Cytosol DNA DNA Replication Dogs Female Fluorescent Antibody Technique, Indirect Humans Melatonin Mice Rats Subcellular Fractions
spellingShingle	3T3 mouse fibroblasts Actin Bromodeoxyuridine Immunofluorescence Melatonin Serotonin actin broxuridine melatonin serotonin tubulin animal cell article cattle cell cycle cell strain 3t3 cellular distribution controlled study dna synthesis dog granulosa cell hela cell human human cell kidney cell monocyte mouse neuroblastoma cell nonhuman rat spleen cell teratocarcinoma Animals Bromodeoxyuridine Cattle Cell Culture Techniques Cell Cycle Cell Line Cell Nucleus Cytoskeletal Proteins Cytosol DNA DNA Replication Dogs Female Fluorescent Antibody Technique, Indirect Humans Melatonin Mice Rats Subcellular Fractions Finocchiaro, L.M.E. Glikin, G.C. Intracellular melatonin distribution in cultured cell lines
topic_facet	3T3 mouse fibroblasts Actin Bromodeoxyuridine Immunofluorescence Melatonin Serotonin actin broxuridine melatonin serotonin tubulin animal cell article cattle cell cycle cell strain 3t3 cellular distribution controlled study dna synthesis dog granulosa cell hela cell human human cell kidney cell monocyte mouse neuroblastoma cell nonhuman rat spleen cell teratocarcinoma Animals Bromodeoxyuridine Cattle Cell Culture Techniques Cell Cycle Cell Line Cell Nucleus Cytoskeletal Proteins Cytosol DNA DNA Replication Dogs Female Fluorescent Antibody Technique, Indirect Humans Melatonin Mice Rats Subcellular Fractions
description	A specific antibody combined with a fluorescein-labeled immunoglobulin was used to investigate the topographic distribution of melatonin in a variety of cells of different origins. Positive identification of both nuclear and cytosolic melatonin was confirmed in all the tested cells: Swiss 3T3 mouse fibroblasts, BCG1 bovine granulosa, NB41A3 mouse neuroblastoma, F9 mouse teratocarcinoma, MDCK normal canine kidney derived and human HeLa cell lines, as well as in human peripheral blood mononuclear leukocytes and rat splenic cells. In 3T3 mouse fibroblasts melatonin immunofluorescence partially colocalized with actin and serotonin immunostaining, but not with tubulin or actin stress fibers. Several distinct patterns of subcellular melatonin distribution, different from the bromodeoxyuridine-labeled replication profiles, have been discerned throughout the cell cycle of synchronized 3T3 cells. In addition, synchronized 3T3 mouse fibroblasts cultured in the presence of 10-3 M melatonin progressed more slowly through the cell cycle than control cells. These results suggest that melatonin may interact directly with nuclear and cytoskeletal structures probably affecting different cell functions such as cell cycle control, subcellular organization, and genome stability.
format	JOUR
author	Finocchiaro, L.M.E. Glikin, G.C.
author_facet	Finocchiaro, L.M.E. Glikin, G.C.
author_sort	Finocchiaro, L.M.E.
title	Intracellular melatonin distribution in cultured cell lines
title_short	Intracellular melatonin distribution in cultured cell lines
title_full	Intracellular melatonin distribution in cultured cell lines
title_fullStr	Intracellular melatonin distribution in cultured cell lines
title_full_unstemmed	Intracellular melatonin distribution in cultured cell lines
title_sort	intracellular melatonin distribution in cultured cell lines
url	http://hdl.handle.net/20.500.12110/paper_07423098_v24_n1_p22_Finocchiaro
work_keys_str_mv	AT finocchiarolme intracellularmelatonindistributioninculturedcelllines AT glikingc intracellularmelatonindistributioninculturedcelllines
_version_	1807316634524712960

Intracellular melatonin distribution in cultured cell lines

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