Modulating surface density of proteins via caged surfaces and controlled light exposure
We demonstrate the possibility of tuning the degree of functionalization of a surface using photoactivatable chemistries and controlled light exposure. A photosensitive organosilane with a protected amine terminal group and a tetraethyleneglycol spacer was synthesized. A o-nitrobenzyl cage was used...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_07437463_v27_n6_p2789_Alvarez |
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todo:paper_07437463_v27_n6_p2789_Alvarez2023-10-03T15:38:44Z Modulating surface density of proteins via caged surfaces and controlled light exposure Álvarez, M. Alonso, J.M. Filevich, O. Bhagawati, M. Etchenique, R. Piehler, J. Del Campo, A. Amine functionality Degree of functionalization Density control Ligand density Light exposure Organosilanes Photo-tunable Photoactivatable Protein density Protein layers Protein micropatterns Protein surface Silica substrate Surface density Terminal groups Underlying layers Light sensitive materials Organic compounds Photosensitivity Silica Proteins photosensitizing agent protein article chemistry light photochemistry surface property synthesis Light Photochemical Processes Photosensitizing Agents Proteins Surface Properties We demonstrate the possibility of tuning the degree of functionalization of a surface using photoactivatable chemistries and controlled light exposure. A photosensitive organosilane with a protected amine terminal group and a tetraethyleneglycol spacer was synthesized. A o-nitrobenzyl cage was used as the photoremovable group to cage the amine functionality. Surfaces with phototunable amine densities were generated by controlled irradiation of silica substrates modified with the photosensitive anchor. Protein layers with different densities could be obtained by successive coupling and assembly steps. Protein surface concentrations were quantified by reflectance interference. Our results demonstrate that the protein density correlates with the photogenerated ligand density. The density control was proved over four coupling steps (biotin, SAv, BTtris-NTA, MBP, or GFP), indicating that the interactions between underlying layer and soluble targets are highly specific and the immobilized targets at the four levels maintain their full functionality. Protein micropatterns with a gradient of protein density were also obtained. © 2011 American Chemical Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07437463_v27_n6_p2789_Alvarez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amine functionality Degree of functionalization Density control Ligand density Light exposure Organosilanes Photo-tunable Photoactivatable Protein density Protein layers Protein micropatterns Protein surface Silica substrate Surface density Terminal groups Underlying layers Light sensitive materials Organic compounds Photosensitivity Silica Proteins photosensitizing agent protein article chemistry light photochemistry surface property synthesis Light Photochemical Processes Photosensitizing Agents Proteins Surface Properties |
| spellingShingle |
Amine functionality Degree of functionalization Density control Ligand density Light exposure Organosilanes Photo-tunable Photoactivatable Protein density Protein layers Protein micropatterns Protein surface Silica substrate Surface density Terminal groups Underlying layers Light sensitive materials Organic compounds Photosensitivity Silica Proteins photosensitizing agent protein article chemistry light photochemistry surface property synthesis Light Photochemical Processes Photosensitizing Agents Proteins Surface Properties Álvarez, M. Alonso, J.M. Filevich, O. Bhagawati, M. Etchenique, R. Piehler, J. Del Campo, A. Modulating surface density of proteins via caged surfaces and controlled light exposure |
| topic_facet |
Amine functionality Degree of functionalization Density control Ligand density Light exposure Organosilanes Photo-tunable Photoactivatable Protein density Protein layers Protein micropatterns Protein surface Silica substrate Surface density Terminal groups Underlying layers Light sensitive materials Organic compounds Photosensitivity Silica Proteins photosensitizing agent protein article chemistry light photochemistry surface property synthesis Light Photochemical Processes Photosensitizing Agents Proteins Surface Properties |
| description |
We demonstrate the possibility of tuning the degree of functionalization of a surface using photoactivatable chemistries and controlled light exposure. A photosensitive organosilane with a protected amine terminal group and a tetraethyleneglycol spacer was synthesized. A o-nitrobenzyl cage was used as the photoremovable group to cage the amine functionality. Surfaces with phototunable amine densities were generated by controlled irradiation of silica substrates modified with the photosensitive anchor. Protein layers with different densities could be obtained by successive coupling and assembly steps. Protein surface concentrations were quantified by reflectance interference. Our results demonstrate that the protein density correlates with the photogenerated ligand density. The density control was proved over four coupling steps (biotin, SAv, BTtris-NTA, MBP, or GFP), indicating that the interactions between underlying layer and soluble targets are highly specific and the immobilized targets at the four levels maintain their full functionality. Protein micropatterns with a gradient of protein density were also obtained. © 2011 American Chemical Society. |
| format |
JOUR |
| author |
Álvarez, M. Alonso, J.M. Filevich, O. Bhagawati, M. Etchenique, R. Piehler, J. Del Campo, A. |
| author_facet |
Álvarez, M. Alonso, J.M. Filevich, O. Bhagawati, M. Etchenique, R. Piehler, J. Del Campo, A. |
| author_sort |
Álvarez, M. |
| title |
Modulating surface density of proteins via caged surfaces and controlled light exposure |
| title_short |
Modulating surface density of proteins via caged surfaces and controlled light exposure |
| title_full |
Modulating surface density of proteins via caged surfaces and controlled light exposure |
| title_fullStr |
Modulating surface density of proteins via caged surfaces and controlled light exposure |
| title_full_unstemmed |
Modulating surface density of proteins via caged surfaces and controlled light exposure |
| title_sort |
modulating surface density of proteins via caged surfaces and controlled light exposure |
| url |
http://hdl.handle.net/20.500.12110/paper_07437463_v27_n6_p2789_Alvarez |
| work_keys_str_mv |
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| _version_ |
1807321741063618560 |