Naphthalene detection by a bioluminescence sensor applied to wastewater samples

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A bioluminescence detection system using Pseudomonas fluorescens HK44 was applied for naphthalene measurement in the range 0-0.5mgl-1, lower than the maximum of 1mgl-1 recommended by EPA. Diluted sample injections of naphthalene were added to 0.2 or 0.4gl-1 cellular concentrations used in the detect...

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Autores principales: Valdman, E., Battaglini, F., Leite, S.G.F., Valdman, B.
Formato: JOUR
Materias:
Bioluminescence
Naphthalene
On-line detection
Pseudomonas fluorescens HK44
Wastewater
Bioassay
Biological water treatment
Biosensors
Contamination
Effluents
Genetic engineering
Petroleum industry
pH effects
Polycyclic aromatic hydrocarbons
Wastewater treatment
Yeast
Catabolism
Genetically engineered microorganisms (GEM)
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09254005_v103_n1-2_p7_Valdman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09254005_v103_n1-2_p7_Valdman
record_format dspace
spelling todo:paper_09254005_v103_n1-2_p7_Valdman2023-10-03T15:46:10Z Naphthalene detection by a bioluminescence sensor applied to wastewater samples Valdman, E. Battaglini, F. Leite, S.G.F. Valdman, B. Bioluminescence Naphthalene On-line detection Pseudomonas fluorescens HK44 Wastewater Bioassay Biological water treatment Biosensors Contamination Effluents Genetic engineering Naphthalene Petroleum industry pH effects Polycyclic aromatic hydrocarbons Wastewater Wastewater treatment Yeast Catabolism Genetically engineered microorganisms (GEM) On-line detection Pseudomonas fluorescens HK44 Bioluminescence A bioluminescence detection system using Pseudomonas fluorescens HK44 was applied for naphthalene measurement in the range 0-0.5mgl-1, lower than the maximum of 1mgl-1 recommended by EPA. Diluted sample injections of naphthalene were added to 0.2 or 0.4gl-1 cellular concentrations used in the detection medium. The lowest cell density (0.2gl -1) exhibited a linear response up to naphthalene concentration of about 0.4mgl-1 being selected to be used in the subsequent experiments. The same system was applied to samples of influent and effluent streams of a biological wastewater treatment from a petroleum industry containing 2.4 and 1.1mg hydrocarbonsl-1, respectively, including naphthalene. The output signal of the biosensor system for the influent stream sample was fully compatible with the results obtained for the naphthalene solutions. Results obtained with the effluent sample showed that other contaminants than naphthalene may influence the biosensor response and are under investigations. © 2004 Elsevier B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09254005_v103_n1-2_p7_Valdman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bioluminescence
Naphthalene
On-line detection
Pseudomonas fluorescens HK44
Wastewater
Bioassay
Biological water treatment
Biosensors
Contamination
Effluents
Genetic engineering
Naphthalene
Petroleum industry
pH effects
Polycyclic aromatic hydrocarbons
Wastewater
Wastewater treatment
Yeast
Catabolism
Genetically engineered microorganisms (GEM)
On-line detection
Pseudomonas fluorescens HK44
Bioluminescence
spellingShingle Bioluminescence
Naphthalene
On-line detection
Pseudomonas fluorescens HK44
Wastewater
Bioassay
Biological water treatment
Biosensors
Contamination
Effluents
Genetic engineering
Naphthalene
Petroleum industry
pH effects
Polycyclic aromatic hydrocarbons
Wastewater
Wastewater treatment
Yeast
Catabolism
Genetically engineered microorganisms (GEM)
On-line detection
Pseudomonas fluorescens HK44
Bioluminescence
Valdman, E.
Battaglini, F.
Leite, S.G.F.
Valdman, B.
Naphthalene detection by a bioluminescence sensor applied to wastewater samples
topic_facet Bioluminescence
Naphthalene
On-line detection
Pseudomonas fluorescens HK44
Wastewater
Bioassay
Biological water treatment
Biosensors
Contamination
Effluents
Genetic engineering
Naphthalene
Petroleum industry
pH effects
Polycyclic aromatic hydrocarbons
Wastewater
Wastewater treatment
Yeast
Catabolism
Genetically engineered microorganisms (GEM)
On-line detection
Pseudomonas fluorescens HK44
Bioluminescence
description A bioluminescence detection system using Pseudomonas fluorescens HK44 was applied for naphthalene measurement in the range 0-0.5mgl-1, lower than the maximum of 1mgl-1 recommended by EPA. Diluted sample injections of naphthalene were added to 0.2 or 0.4gl-1 cellular concentrations used in the detection medium. The lowest cell density (0.2gl -1) exhibited a linear response up to naphthalene concentration of about 0.4mgl-1 being selected to be used in the subsequent experiments. The same system was applied to samples of influent and effluent streams of a biological wastewater treatment from a petroleum industry containing 2.4 and 1.1mg hydrocarbonsl-1, respectively, including naphthalene. The output signal of the biosensor system for the influent stream sample was fully compatible with the results obtained for the naphthalene solutions. Results obtained with the effluent sample showed that other contaminants than naphthalene may influence the biosensor response and are under investigations. © 2004 Elsevier B.V. All rights reserved.
format JOUR
author Valdman, E.
Battaglini, F.
Leite, S.G.F.
Valdman, B.
author_facet Valdman, E.
Battaglini, F.
Leite, S.G.F.
Valdman, B.
author_sort Valdman, E.
title Naphthalene detection by a bioluminescence sensor applied to wastewater samples
title_short Naphthalene detection by a bioluminescence sensor applied to wastewater samples
title_full Naphthalene detection by a bioluminescence sensor applied to wastewater samples
title_fullStr Naphthalene detection by a bioluminescence sensor applied to wastewater samples
title_full_unstemmed Naphthalene detection by a bioluminescence sensor applied to wastewater samples
title_sort naphthalene detection by a bioluminescence sensor applied to wastewater samples
url http://hdl.handle.net/20.500.12110/paper_09254005_v103_n1-2_p7_Valdman
work_keys_str_mv AT valdmane naphthalenedetectionbyabioluminescencesensorappliedtowastewatersamples
AT battaglinif naphthalenedetectionbyabioluminescencesensorappliedtowastewatersamples
AT leitesgf naphthalenedetectionbyabioluminescencesensorappliedtowastewatersamples
AT valdmanb naphthalenedetectionbyabioluminescencesensorappliedtowastewatersamples
_version_ 1807315142681034752

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