The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis
We analyzed the influence of presenilins on the genetic cascades that control neuronal differentiation in Xenopus embryos. Resembling sonic hedgehog (shh) overexpression, presenilin mRNA injection reduced the number of N-tubulin+ primary neurons and modulated Gli3 and Zic2 according to their roles i...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_09254773_v107_n1-2_p119_Paganelli |
| Aporte de: |
| id |
todo:paper_09254773_v107_n1-2_p119_Paganelli |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_09254773_v107_n1-2_p119_Paganelli2023-10-03T15:46:18Z The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis Paganelli, A.R. Ocaña, O. Prat, M.I. Franco, P.G. López, S.L. Morelli, L. Adamo, A.M. Riccomagno, M.M. Matsubara, E. Shoji, M. Affranchino, J.L. Castaño, E.M. Carrasco, A.E. Apoptosis Morpholino oligonucleotide Presenilin Primary neurogenesis Proliferation Sonic hedgehog Xenopus laevis antisense oligonucleotide messenger RNA presenilin 1 sonic hedgehog protein Alzheimer disease apoptosis article controlled study embryo gene expression regulation immunohistochemistry nervous system development nonhuman priority journal reverse transcription polymerase chain reaction Xenopus Xenopus We analyzed the influence of presenilins on the genetic cascades that control neuronal differentiation in Xenopus embryos. Resembling sonic hedgehog (shh) overexpression, presenilin mRNA injection reduced the number of N-tubulin+ primary neurons and modulated Gli3 and Zic2 according to their roles in activating and repressing primary neurogenesis, respectively. Presenilin increased shh expression within its normal domain, mainly in the floor plate, whereas an antisense X-presenilin-α morpholino oligonucleotide reduced shh expression. Both shh and presenilin promoted cell proliferation and apoptosis, but the effects of shh were widely distributed, while those resulting from presenilin injection coincided with the range of shh signaling. We suggest that presenilin may modulate primary neurogenesis, proliferation, and apoptosis in the neural plate, through the enhancement of shh signaling. Copyright © 2001 Elsevier Science Ireland Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09254773_v107_n1-2_p119_Paganelli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Morpholino oligonucleotide Presenilin Primary neurogenesis Proliferation Sonic hedgehog Xenopus laevis antisense oligonucleotide messenger RNA presenilin 1 sonic hedgehog protein Alzheimer disease apoptosis article controlled study embryo gene expression regulation immunohistochemistry nervous system development nonhuman priority journal reverse transcription polymerase chain reaction Xenopus Xenopus |
| spellingShingle |
Apoptosis Morpholino oligonucleotide Presenilin Primary neurogenesis Proliferation Sonic hedgehog Xenopus laevis antisense oligonucleotide messenger RNA presenilin 1 sonic hedgehog protein Alzheimer disease apoptosis article controlled study embryo gene expression regulation immunohistochemistry nervous system development nonhuman priority journal reverse transcription polymerase chain reaction Xenopus Xenopus Paganelli, A.R. Ocaña, O. Prat, M.I. Franco, P.G. López, S.L. Morelli, L. Adamo, A.M. Riccomagno, M.M. Matsubara, E. Shoji, M. Affranchino, J.L. Castaño, E.M. Carrasco, A.E. The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| topic_facet |
Apoptosis Morpholino oligonucleotide Presenilin Primary neurogenesis Proliferation Sonic hedgehog Xenopus laevis antisense oligonucleotide messenger RNA presenilin 1 sonic hedgehog protein Alzheimer disease apoptosis article controlled study embryo gene expression regulation immunohistochemistry nervous system development nonhuman priority journal reverse transcription polymerase chain reaction Xenopus Xenopus |
| description |
We analyzed the influence of presenilins on the genetic cascades that control neuronal differentiation in Xenopus embryos. Resembling sonic hedgehog (shh) overexpression, presenilin mRNA injection reduced the number of N-tubulin+ primary neurons and modulated Gli3 and Zic2 according to their roles in activating and repressing primary neurogenesis, respectively. Presenilin increased shh expression within its normal domain, mainly in the floor plate, whereas an antisense X-presenilin-α morpholino oligonucleotide reduced shh expression. Both shh and presenilin promoted cell proliferation and apoptosis, but the effects of shh were widely distributed, while those resulting from presenilin injection coincided with the range of shh signaling. We suggest that presenilin may modulate primary neurogenesis, proliferation, and apoptosis in the neural plate, through the enhancement of shh signaling. Copyright © 2001 Elsevier Science Ireland Ltd. |
| format |
JOUR |
| author |
Paganelli, A.R. Ocaña, O. Prat, M.I. Franco, P.G. López, S.L. Morelli, L. Adamo, A.M. Riccomagno, M.M. Matsubara, E. Shoji, M. Affranchino, J.L. Castaño, E.M. Carrasco, A.E. |
| author_facet |
Paganelli, A.R. Ocaña, O. Prat, M.I. Franco, P.G. López, S.L. Morelli, L. Adamo, A.M. Riccomagno, M.M. Matsubara, E. Shoji, M. Affranchino, J.L. Castaño, E.M. Carrasco, A.E. |
| author_sort |
Paganelli, A.R. |
| title |
The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| title_short |
The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| title_full |
The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| title_fullStr |
The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| title_full_unstemmed |
The Alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in Xenopus primary neurogenesis |
| title_sort |
alzheimer-related gene presenilin-1 facilitates sonic hedgehog expression in xenopus primary neurogenesis |
| url |
http://hdl.handle.net/20.500.12110/paper_09254773_v107_n1-2_p119_Paganelli |
| work_keys_str_mv |
AT paganelliar thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT ocanao thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT pratmi thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT francopg thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT lopezsl thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT morellil thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT adamoam thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT riccomagnomm thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT matsubarae thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT shojim thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT affranchinojl thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT castanoem thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT carrascoae thealzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT paganelliar alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT ocanao alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT pratmi alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT francopg alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT lopezsl alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT morellil alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT adamoam alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT riccomagnomm alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT matsubarae alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT shojim alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT affranchinojl alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT castanoem alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis AT carrascoae alzheimerrelatedgenepresenilin1facilitatessonichedgehogexpressioninxenopusprimaryneurogenesis |
| _version_ |
1807323833939525632 |