Partially purified leaf extracts of Melia azedarach L. inhibit Tacaribe virus growth in neonatal mice

Neonatal mice, inoculated intraperitoneally with 103 PFU of Tacaribe virus, died after developing a typical encephalitis. The administration of partially purified extracts of Melia azedarach L. (Ma) fresh green leaves, in seven doses by the same route, reduced virus spread which normally involves th...

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Autores principales: Coulombie, F.C., Andrei, G.M., Laguens, R.P., De Torres, R.A., Coto, C.E.
Formato: JOUR
Lenguaje:English
Materias:
interferon
melia azedarach extract
plant extract
unclassified drug
animal experiment
animal model
arenavirus
article
brain
immune response
intraperitoneal drug administration
kidney
liver
mouse
newborn
nonhuman
virus concentration
virus infection
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0951418X_v6_n1_p15_Coulombie
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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record_format dspace
spelling todo:paper_0951418X_v6_n1_p15_Coulombie2023-10-03T15:50:39Z Partially purified leaf extracts of Melia azedarach L. inhibit Tacaribe virus growth in neonatal mice Coulombie, F.C. Andrei, G.M. Laguens, R.P. De Torres, R.A. Coto, C.E. interferon melia azedarach extract plant extract unclassified drug animal experiment animal model arenavirus article brain immune response intraperitoneal drug administration kidney liver mouse newborn nonhuman virus concentration virus infection Neonatal mice, inoculated intraperitoneally with 103 PFU of Tacaribe virus, died after developing a typical encephalitis. The administration of partially purified extracts of Melia azedarach L. (Ma) fresh green leaves, in seven doses by the same route, reduced virus spread which normally involves the kidneys, liver and brain. Mortality fell to 50% in treated mice and viral titres in brain were at least 3 logs lower than those of untreated mice. Surviving mice mounted a humoral immune response. High levels of neutralizing antibodies were present up to 60 days post-infection when the animals were killed. Ma administration exerts a marked decrease in circulating interferon levels of infected mice. Thus, at day 6 post-infection the amount of acid resistant interferon in treated animals was eight times lower than in those untreated. The antiviral effect of Ma in vivo is clearly stated in this murine-arenavirus model. Fil:Coulombie, F.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Andrei, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0951418X_v6_n1_p15_Coulombie
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language English
orig_language_str_mv English
topic interferon
melia azedarach extract
plant extract
unclassified drug
animal experiment
animal model
arenavirus
article
brain
immune response
intraperitoneal drug administration
kidney
liver
mouse
newborn
nonhuman
virus concentration
virus infection
spellingShingle interferon
melia azedarach extract
plant extract
unclassified drug
animal experiment
animal model
arenavirus
article
brain
immune response
intraperitoneal drug administration
kidney
liver
mouse
newborn
nonhuman
virus concentration
virus infection
Coulombie, F.C.
Andrei, G.M.
Laguens, R.P.
De Torres, R.A.
Coto, C.E.
Partially purified leaf extracts of Melia azedarach L. inhibit Tacaribe virus growth in neonatal mice
topic_facet interferon
melia azedarach extract
plant extract
unclassified drug
animal experiment
animal model
arenavirus
article
brain
immune response
intraperitoneal drug administration
kidney
liver
mouse
newborn
nonhuman
virus concentration
virus infection
description Neonatal mice, inoculated intraperitoneally with 103 PFU of Tacaribe virus, died after developing a typical encephalitis. The administration of partially purified extracts of Melia azedarach L. (Ma) fresh green leaves, in seven doses by the same route, reduced virus spread which normally involves the kidneys, liver and brain. Mortality fell to 50% in treated mice and viral titres in brain were at least 3 logs lower than those of untreated mice. Surviving mice mounted a humoral immune response. High levels of neutralizing antibodies were present up to 60 days post-infection when the animals were killed. Ma administration exerts a marked decrease in circulating interferon levels of infected mice. Thus, at day 6 post-infection the amount of acid resistant interferon in treated animals was eight times lower than in those untreated. The antiviral effect of Ma in vivo is clearly stated in this murine-arenavirus model.
format JOUR
author Coulombie, F.C.
Andrei, G.M.
Laguens, R.P.
De Torres, R.A.
Coto, C.E.
author_facet Coulombie, F.C.
Andrei, G.M.
Laguens, R.P.
De Torres, R.A.
Coto, C.E.
author_sort Coulombie, F.C.
title Partially purified leaf extracts of Melia azedarach L. inhibit Tacaribe virus growth in neonatal mice
title_short Partially purified leaf extracts of Melia azedarach L. inhibit Tacaribe virus growth in neonatal mice
title_full Partially purified leaf extracts of Melia azedarach L. inhibit Tacaribe virus growth in neonatal mice
title_fullStr Partially purified leaf extracts of Melia azedarach L. inhibit Tacaribe virus growth in neonatal mice
title_full_unstemmed Partially purified leaf extracts of Melia azedarach L. inhibit Tacaribe virus growth in neonatal mice
title_sort partially purified leaf extracts of melia azedarach l. inhibit tacaribe virus growth in neonatal mice
url http://hdl.handle.net/20.500.12110/paper_0951418X_v6_n1_p15_Coulombie
work_keys_str_mv AT coulombiefc partiallypurifiedleafextractsofmeliaazedarachlinhibittacaribevirusgrowthinneonatalmice
AT andreigm partiallypurifiedleafextractsofmeliaazedarachlinhibittacaribevirusgrowthinneonatalmice
AT laguensrp partiallypurifiedleafextractsofmeliaazedarachlinhibittacaribevirusgrowthinneonatalmice
AT detorresra partiallypurifiedleafextractsofmeliaazedarachlinhibittacaribevirusgrowthinneonatalmice
AT cotoce partiallypurifiedleafextractsofmeliaazedarachlinhibittacaribevirusgrowthinneonatalmice
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