Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice

The dopamine D4 receptor (D4R) has received considerable interest because of its higher affinity for atypical antipsychotics, the extremely polymorphic nature of the human gene and the genetic association with attention deficit and hyperactivity disorder (ADHD). Several efforts have been undertaken...

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Autores principales:	Noaín, D., Avale, M.E., Wedemeyer, C., Calvo, D., Peper, M., Rubinstein, M.
Formato:	JOUR
Materias:	Anterior olfactory nucleus Green fluorescent protein Lateral parabrachial nucleus Prefrontal cortex Ventral pallidum dopamine 4 receptor enhanced green fluorescent protein animal cell animal experiment animal tissue article bacterial artificial chromosome brain nerve cell cell labeling cingulate gyrus corpus striatum dopaminergic transmission female gene expression profiling gene identification globus pallidus hippocampus immunohistochemistry limbic cortex male mesencephalon mouse nonhuman olfactory nucleus orbital cortex parabrachial nucleus prefrontal cortex priority journal receptor gene transgenic mouse Animals Brain Chromosomes, Artificial, Bacterial Embryo Green Fluorescent Proteins Immunohistochemistry Male Mice Mice, Transgenic Microscopy, Confocal Neurons Patch-Clamp Techniques Receptors, Dopamine D4
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0953816X_v24_n9_p2429_Noain
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_0953816X_v24_n9_p2429_Noain2023-10-03T15:51:17Z Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice Noaín, D. Avale, M.E. Wedemeyer, C. Calvo, D. Peper, M. Rubinstein, M. Anterior olfactory nucleus Green fluorescent protein Lateral parabrachial nucleus Prefrontal cortex Ventral pallidum dopamine 4 receptor enhanced green fluorescent protein animal cell animal experiment animal tissue article bacterial artificial chromosome brain nerve cell cell labeling cingulate gyrus corpus striatum dopaminergic transmission female gene expression profiling gene identification globus pallidus hippocampus immunohistochemistry limbic cortex male mesencephalon mouse nonhuman olfactory nucleus orbital cortex parabrachial nucleus prefrontal cortex priority journal receptor gene transgenic mouse Animals Brain Chromosomes, Artificial, Bacterial Embryo Green Fluorescent Proteins Immunohistochemistry Male Mice Mice, Transgenic Microscopy, Confocal Neurons Patch-Clamp Techniques Receptors, Dopamine D4 The dopamine D4 receptor (D4R) has received considerable interest because of its higher affinity for atypical antipsychotics, the extremely polymorphic nature of the human gene and the genetic association with attention deficit and hyperactivity disorder (ADHD). Several efforts have been undertaken to determine the D4R expression pattern in the brain using immunohistochemistry, binding autoradiography and in situ hybridization, but the overall published results present large discrepancies. Here, we have explored an alternative genetic approach by studying bacterial artificial chromosome (BAC) transgenic mice that express enhanced green fluorescent protein (EGFP) under the transcriptional control of the mouse dopamine D4 receptor gene (Drd4). Immunohistochemical analysis performed in brain sections of Drd4-EGFP transgenic mice using an anti-EGFP polyclonal antibody showed that transgenic expression was predominant in deep layer neurons of the prefrontal cortex, particularly in the orbital, prelimbic, cingulate and rostral agranular portions. In addition, discrete groups of Drd4-EGFP labelled neurons were observed in the anterior olfactory nucleus, ventral pallidum, and lateral parabrachial nucleus. EGFP was not detected in the striatum, hippocampus or midbrain as described using other techniques. Given the fine specificity of EGFP expression in BAC transgenic mice and the high sensitivity of the EGFP antibody used in this study, our results indicate that Drd4 expression in the adult mouse brain is limited to a more restricted number of areas than previously reported. Its leading expression in the prefrontal cortex supports the importance of the D4R in complex behaviours depending on cortical dopamine (DA) transmission and its possible role in the etiopathophysiology of ADHD. © The Authors (2006). Fil:Noaín, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Avale, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wedemeyer, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Peper, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rubinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0953816X_v24_n9_p2429_Noain
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Anterior olfactory nucleus Green fluorescent protein Lateral parabrachial nucleus Prefrontal cortex Ventral pallidum dopamine 4 receptor enhanced green fluorescent protein animal cell animal experiment animal tissue article bacterial artificial chromosome brain nerve cell cell labeling cingulate gyrus corpus striatum dopaminergic transmission female gene expression profiling gene identification globus pallidus hippocampus immunohistochemistry limbic cortex male mesencephalon mouse nonhuman olfactory nucleus orbital cortex parabrachial nucleus prefrontal cortex priority journal receptor gene transgenic mouse Animals Brain Chromosomes, Artificial, Bacterial Embryo Green Fluorescent Proteins Immunohistochemistry Male Mice Mice, Transgenic Microscopy, Confocal Neurons Patch-Clamp Techniques Receptors, Dopamine D4
spellingShingle	Anterior olfactory nucleus Green fluorescent protein Lateral parabrachial nucleus Prefrontal cortex Ventral pallidum dopamine 4 receptor enhanced green fluorescent protein animal cell animal experiment animal tissue article bacterial artificial chromosome brain nerve cell cell labeling cingulate gyrus corpus striatum dopaminergic transmission female gene expression profiling gene identification globus pallidus hippocampus immunohistochemistry limbic cortex male mesencephalon mouse nonhuman olfactory nucleus orbital cortex parabrachial nucleus prefrontal cortex priority journal receptor gene transgenic mouse Animals Brain Chromosomes, Artificial, Bacterial Embryo Green Fluorescent Proteins Immunohistochemistry Male Mice Mice, Transgenic Microscopy, Confocal Neurons Patch-Clamp Techniques Receptors, Dopamine D4 Noaín, D. Avale, M.E. Wedemeyer, C. Calvo, D. Peper, M. Rubinstein, M. Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice
topic_facet	Anterior olfactory nucleus Green fluorescent protein Lateral parabrachial nucleus Prefrontal cortex Ventral pallidum dopamine 4 receptor enhanced green fluorescent protein animal cell animal experiment animal tissue article bacterial artificial chromosome brain nerve cell cell labeling cingulate gyrus corpus striatum dopaminergic transmission female gene expression profiling gene identification globus pallidus hippocampus immunohistochemistry limbic cortex male mesencephalon mouse nonhuman olfactory nucleus orbital cortex parabrachial nucleus prefrontal cortex priority journal receptor gene transgenic mouse Animals Brain Chromosomes, Artificial, Bacterial Embryo Green Fluorescent Proteins Immunohistochemistry Male Mice Mice, Transgenic Microscopy, Confocal Neurons Patch-Clamp Techniques Receptors, Dopamine D4
description	The dopamine D4 receptor (D4R) has received considerable interest because of its higher affinity for atypical antipsychotics, the extremely polymorphic nature of the human gene and the genetic association with attention deficit and hyperactivity disorder (ADHD). Several efforts have been undertaken to determine the D4R expression pattern in the brain using immunohistochemistry, binding autoradiography and in situ hybridization, but the overall published results present large discrepancies. Here, we have explored an alternative genetic approach by studying bacterial artificial chromosome (BAC) transgenic mice that express enhanced green fluorescent protein (EGFP) under the transcriptional control of the mouse dopamine D4 receptor gene (Drd4). Immunohistochemical analysis performed in brain sections of Drd4-EGFP transgenic mice using an anti-EGFP polyclonal antibody showed that transgenic expression was predominant in deep layer neurons of the prefrontal cortex, particularly in the orbital, prelimbic, cingulate and rostral agranular portions. In addition, discrete groups of Drd4-EGFP labelled neurons were observed in the anterior olfactory nucleus, ventral pallidum, and lateral parabrachial nucleus. EGFP was not detected in the striatum, hippocampus or midbrain as described using other techniques. Given the fine specificity of EGFP expression in BAC transgenic mice and the high sensitivity of the EGFP antibody used in this study, our results indicate that Drd4 expression in the adult mouse brain is limited to a more restricted number of areas than previously reported. Its leading expression in the prefrontal cortex supports the importance of the D4R in complex behaviours depending on cortical dopamine (DA) transmission and its possible role in the etiopathophysiology of ADHD. © The Authors (2006).
format	JOUR
author	Noaín, D. Avale, M.E. Wedemeyer, C. Calvo, D. Peper, M. Rubinstein, M.
author_facet	Noaín, D. Avale, M.E. Wedemeyer, C. Calvo, D. Peper, M. Rubinstein, M.
author_sort	Noaín, D.
title	Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice
title_short	Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice
title_full	Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice
title_fullStr	Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice
title_full_unstemmed	Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice
title_sort	identification of brain neurons expressing the dopamine d4 receptor gene using bac transgenic mice
url	http://hdl.handle.net/20.500.12110/paper_0953816X_v24_n9_p2429_Noain
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_version_	1807314669200736256

Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice

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