Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis

Ri T-DNA transformed roots have been used effectively in studying the interaction between various plant hosts and arbuscular mycorrhizal (AM) fungi. We investigated the in vitro monoxenic symbiosis between the AM fungus Glomus intraradices and transformed soybean roots (TSRs). Comparisons were made...

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Autores principales: Fernández, L., Silvani, V., Bompadre, J., Pérgola, M., Godeas, A.
Formato: JOUR
Materias:
Glomus intraradices
Monoxenic culture
Transformed soybean roots
Absorbing structure
Am fungus
Arbuscular mycorrhizal fungi
Arbuscular mycorrhizal symbiosis
Characteristic development
Cortical cells
Glycine max
In-plants
In-vitro
Intensity values
Plant hosts
Soybean roots
Whole plants
Amplitude modulation
Nitrogen fixation
Nucleic acids
Plants (botany)
Cell culture
Arbuscular
Bacteria (microorganisms)
Fungi
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09593993_v25_n10_p1857_Fernandez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_09593993_v25_n10_p1857_Fernandez
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spelling todo:paper_09593993_v25_n10_p1857_Fernandez2023-10-03T15:52:52Z Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis Fernández, L. Silvani, V. Bompadre, J. Pérgola, M. Godeas, A. Glomus intraradices Monoxenic culture Transformed soybean roots Absorbing structure Am fungus Arbuscular mycorrhizal fungi Arbuscular mycorrhizal symbiosis Characteristic development Cortical cells Glomus intraradices Glycine max In-plants In-vitro Intensity values Plant hosts Soybean roots Whole plants Amplitude modulation Nitrogen fixation Nucleic acids Plants (botany) Cell culture Arbuscular Bacteria (microorganisms) Fungi Glomus intraradices Glycine max Ri T-DNA transformed roots have been used effectively in studying the interaction between various plant hosts and arbuscular mycorrhizal (AM) fungi. We investigated the in vitro monoxenic symbiosis between the AM fungus Glomus intraradices and transformed soybean roots (TSRs). Comparisons were made between TSR system and plants of the same genotype. The extraradical fungal structures generated in vitro culture showed normal development. Straight runner hyphae branched into short simple branched absorbing structures and spores were initiated. AM symbiosis was confirmed by the presence of arbuscules and vesicles in cortical cells of the TSRs. The frequency of intraradical colonization in TSRs was higher than in plants grown in soil, whereas the intensity values of intraradical colonization in TSR cultures were similar to those in whole plants. These results show that TSR cultures were able to support the growth and characteristic development of G. intraradices. © 2009 Springer Science+Business Media B.V. Fil:Fernández, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Silvani, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bompadre, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérgola, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09593993_v25_n10_p1857_Fernandez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Glomus intraradices
Monoxenic culture
Transformed soybean roots
Absorbing structure
Am fungus
Arbuscular mycorrhizal fungi
Arbuscular mycorrhizal symbiosis
Characteristic development
Cortical cells
Glomus intraradices
Glycine max
In-plants
In-vitro
Intensity values
Plant hosts
Soybean roots
Whole plants
Amplitude modulation
Nitrogen fixation
Nucleic acids
Plants (botany)
Cell culture
Arbuscular
Bacteria (microorganisms)
Fungi
Glomus intraradices
Glycine max
spellingShingle Glomus intraradices
Monoxenic culture
Transformed soybean roots
Absorbing structure
Am fungus
Arbuscular mycorrhizal fungi
Arbuscular mycorrhizal symbiosis
Characteristic development
Cortical cells
Glomus intraradices
Glycine max
In-plants
In-vitro
Intensity values
Plant hosts
Soybean roots
Whole plants
Amplitude modulation
Nitrogen fixation
Nucleic acids
Plants (botany)
Cell culture
Arbuscular
Bacteria (microorganisms)
Fungi
Glomus intraradices
Glycine max
Fernández, L.
Silvani, V.
Bompadre, J.
Pérgola, M.
Godeas, A.
Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
topic_facet Glomus intraradices
Monoxenic culture
Transformed soybean roots
Absorbing structure
Am fungus
Arbuscular mycorrhizal fungi
Arbuscular mycorrhizal symbiosis
Characteristic development
Cortical cells
Glomus intraradices
Glycine max
In-plants
In-vitro
Intensity values
Plant hosts
Soybean roots
Whole plants
Amplitude modulation
Nitrogen fixation
Nucleic acids
Plants (botany)
Cell culture
Arbuscular
Bacteria (microorganisms)
Fungi
Glomus intraradices
Glycine max
description Ri T-DNA transformed roots have been used effectively in studying the interaction between various plant hosts and arbuscular mycorrhizal (AM) fungi. We investigated the in vitro monoxenic symbiosis between the AM fungus Glomus intraradices and transformed soybean roots (TSRs). Comparisons were made between TSR system and plants of the same genotype. The extraradical fungal structures generated in vitro culture showed normal development. Straight runner hyphae branched into short simple branched absorbing structures and spores were initiated. AM symbiosis was confirmed by the presence of arbuscules and vesicles in cortical cells of the TSRs. The frequency of intraradical colonization in TSRs was higher than in plants grown in soil, whereas the intensity values of intraradical colonization in TSR cultures were similar to those in whole plants. These results show that TSR cultures were able to support the growth and characteristic development of G. intraradices. © 2009 Springer Science+Business Media B.V.
format JOUR
author Fernández, L.
Silvani, V.
Bompadre, J.
Pérgola, M.
Godeas, A.
author_facet Fernández, L.
Silvani, V.
Bompadre, J.
Pérgola, M.
Godeas, A.
author_sort Fernández, L.
title Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
title_short Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
title_full Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
title_fullStr Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
title_full_unstemmed Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
title_sort transformed soybean (glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
url http://hdl.handle.net/20.500.12110/paper_09593993_v25_n10_p1857_Fernandez
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AT pergolam transformedsoybeanglycinemaxrootsasatoolforthestudyofthearbuscularmycorrhizalsymbiosis
AT godeasa transformedsoybeanglycinemaxrootsasatoolforthestudyofthearbuscularmycorrhizalsymbiosis
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