Effect of a NADPH generating system on the steroidogenic response in rat luteal cells

The effect of a NADPH generating system (NADPH-GS) on the function of rat luteal cells was studied. Cells were obtained from pregnant mare serum gonadotropin (PMSG) and human choirionic gonadotropin (hCG) primed immature rats and further incubated with 3 NADPH-GS. This system produced an increase in...

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Autores principales:	Stein, P., Tesone, M.
Formato:	JOUR
Materias:	20α-hydroxy-progesterone, 20α-hydroxy-4-pregnen-3-one 25-hydroxy-cholesterol, 5-cholesten-3β-25-diol pregnenolone progesterone reduced nicotinamide adenine dinucleotide phosphate animal cell article female luteal cell nonhuman priority journal rat steroidogenesis Animal Chorionic Gonadotropin Corpus Luteum Female Gonadotropins, Equine NADP Pregnenolone Progesterone Rats Support, Non-U.S. Gov't Animalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09600760_v41_n2_p145_Stein
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_09600760_v41_n2_p145_Stein
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spelling	todo:paper_09600760_v41_n2_p145_Stein2023-10-03T15:53:26Z Effect of a NADPH generating system on the steroidogenic response in rat luteal cells Stein, P. Tesone, M. 20α-hydroxy-progesterone, 20α-hydroxy-4-pregnen-3-one 25-hydroxy-cholesterol, 5-cholesten-3β-25-diol pregnenolone progesterone reduced nicotinamide adenine dinucleotide phosphate animal cell article female luteal cell nonhuman priority journal rat steroidogenesis Animal Chorionic Gonadotropin Corpus Luteum Female Gonadotropins, Equine NADP Pregnenolone Progesterone Rats Support, Non-U.S. Gov't Animalia The effect of a NADPH generating system (NADPH-GS) on the function of rat luteal cells was studied. Cells were obtained from pregnant mare serum gonadotropin (PMSG) and human choirionic gonadotropin (hCG) primed immature rats and further incubated with 3 NADPH-GS. This system produced an increase in progesterone production and maximal stimulation was achieved at 1 mM NADP+ (10- to 15-fold). This effect was enhanced by addition of luteinizing hormone (LH 0.25 nM) to the incubation medium. On the contrary, insulin (2 nM) inhibited the effect observed with the NADPH-GS. The conversion of progesterone into 20α-hydroxy-progesterone was not responsible for the changes observed. To analyze the site of NADPH action, pregnenolone and progesterone were measured using two inhibitors of steroid biosynthesis: aminoglutethimide and cyanoketone. The results confirm the specific site of action of NADPH at the mitochondrial conversion of cholesterol to pregnenolone. The effect of NADPH-GS was also observed in cultured purified luteal cells suggesting that the action of NADPH could be mediated by a free entry of the cofactor across the luteal cell plasma membrane. It can be concluded that the addition of NADPH improves the luteal cell incubation conditions and contributes to understanding the regulatory action of LH and insulin on the ovarian steroidogenic process. © 1992. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09600760_v41_n2_p145_Stein
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	20α-hydroxy-progesterone, 20α-hydroxy-4-pregnen-3-one 25-hydroxy-cholesterol, 5-cholesten-3β-25-diol pregnenolone progesterone reduced nicotinamide adenine dinucleotide phosphate animal cell article female luteal cell nonhuman priority journal rat steroidogenesis Animal Chorionic Gonadotropin Corpus Luteum Female Gonadotropins, Equine NADP Pregnenolone Progesterone Rats Support, Non-U.S. Gov't Animalia
spellingShingle	20α-hydroxy-progesterone, 20α-hydroxy-4-pregnen-3-one 25-hydroxy-cholesterol, 5-cholesten-3β-25-diol pregnenolone progesterone reduced nicotinamide adenine dinucleotide phosphate animal cell article female luteal cell nonhuman priority journal rat steroidogenesis Animal Chorionic Gonadotropin Corpus Luteum Female Gonadotropins, Equine NADP Pregnenolone Progesterone Rats Support, Non-U.S. Gov't Animalia Stein, P. Tesone, M. Effect of a NADPH generating system on the steroidogenic response in rat luteal cells
topic_facet	20α-hydroxy-progesterone, 20α-hydroxy-4-pregnen-3-one 25-hydroxy-cholesterol, 5-cholesten-3β-25-diol pregnenolone progesterone reduced nicotinamide adenine dinucleotide phosphate animal cell article female luteal cell nonhuman priority journal rat steroidogenesis Animal Chorionic Gonadotropin Corpus Luteum Female Gonadotropins, Equine NADP Pregnenolone Progesterone Rats Support, Non-U.S. Gov't Animalia
description	The effect of a NADPH generating system (NADPH-GS) on the function of rat luteal cells was studied. Cells were obtained from pregnant mare serum gonadotropin (PMSG) and human choirionic gonadotropin (hCG) primed immature rats and further incubated with 3 NADPH-GS. This system produced an increase in progesterone production and maximal stimulation was achieved at 1 mM NADP+ (10- to 15-fold). This effect was enhanced by addition of luteinizing hormone (LH 0.25 nM) to the incubation medium. On the contrary, insulin (2 nM) inhibited the effect observed with the NADPH-GS. The conversion of progesterone into 20α-hydroxy-progesterone was not responsible for the changes observed. To analyze the site of NADPH action, pregnenolone and progesterone were measured using two inhibitors of steroid biosynthesis: aminoglutethimide and cyanoketone. The results confirm the specific site of action of NADPH at the mitochondrial conversion of cholesterol to pregnenolone. The effect of NADPH-GS was also observed in cultured purified luteal cells suggesting that the action of NADPH could be mediated by a free entry of the cofactor across the luteal cell plasma membrane. It can be concluded that the addition of NADPH improves the luteal cell incubation conditions and contributes to understanding the regulatory action of LH and insulin on the ovarian steroidogenic process. © 1992.
format	JOUR
author	Stein, P. Tesone, M.
author_facet	Stein, P. Tesone, M.
author_sort	Stein, P.
title	Effect of a NADPH generating system on the steroidogenic response in rat luteal cells
title_short	Effect of a NADPH generating system on the steroidogenic response in rat luteal cells
title_full	Effect of a NADPH generating system on the steroidogenic response in rat luteal cells
title_fullStr	Effect of a NADPH generating system on the steroidogenic response in rat luteal cells
title_full_unstemmed	Effect of a NADPH generating system on the steroidogenic response in rat luteal cells
title_sort	effect of a nadph generating system on the steroidogenic response in rat luteal cells
url	http://hdl.handle.net/20.500.12110/paper_09600760_v41_n2_p145_Stein
work_keys_str_mv	AT steinp effectofanadphgeneratingsystemonthesteroidogenicresponseinratlutealcells AT tesonem effectofanadphgeneratingsystemonthesteroidogenicresponseinratlutealcells
_version_	1807318199811702784

Effect of a NADPH generating system on the steroidogenic response in rat luteal cells

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