Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin

We have previously described the regulation of interleukin-1 receptor antagonist (IL-1ra) protein secretion and expression by IL-1, glucocorticoids and corticotropin-releasing hormone in monocytes in culture. In the present work, we analyze the direct effect of adrenocorticotropic hormone (ACTH) and...

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Autores principales:	Kovalovsky, D., Pereda, M.P., Stalla, G.K., Holsboer, F., Arzt, E.
Formato:	JOUR
Materias:	β-Endorphin Adrenocorticotropic hormone Glucocorticoids Interleukin- 1 Interleukin-1 receptor antagonist beta endorphin corticotropin interleukin 1 receptor proopiomelanocortin article cytokine production cytokine release dose response hormonal regulation hormone action human human cell hypothalamus hypophysis system immunoregulation monocyte priority journal Adrenocorticotropic Hormone beta-Endorphin Blotting, Northern Cells, Cultured Humans Interleukin 1 Receptor Antagonist Protein Monocytes Pro-Opiomelanocortin Receptors, Interleukin-1 Sialoglycoproteins
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10217401_v6_n5_p367_Kovalovsky
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_10217401_v6_n5_p367_Kovalovsky
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spelling	todo:paper_10217401_v6_n5_p367_Kovalovsky2023-10-03T15:56:40Z Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin Kovalovsky, D. Pereda, M.P. Stalla, G.K. Holsboer, F. Arzt, E. β-Endorphin Adrenocorticotropic hormone Glucocorticoids Interleukin- 1 Interleukin-1 receptor antagonist beta endorphin corticotropin interleukin 1 receptor proopiomelanocortin article cytokine production cytokine release dose response hormonal regulation hormone action human human cell hypothalamus hypophysis system immunoregulation monocyte priority journal Adrenocorticotropic Hormone beta-Endorphin Blotting, Northern Cells, Cultured Humans Interleukin 1 Receptor Antagonist Protein Monocytes Pro-Opiomelanocortin Receptors, Interleukin-1 Sialoglycoproteins We have previously described the regulation of interleukin-1 receptor antagonist (IL-1ra) protein secretion and expression by IL-1, glucocorticoids and corticotropin-releasing hormone in monocytes in culture. In the present work, we analyze the direct effect of adrenocorticotropic hormone (ACTH) and β-endorphin on the expression and secretion of IL-1ra by human monocytes in culture. ACTH exerted a dose-dependent inhibitory effect on lipopolysaccharide (LPS)-induced IL-1ra production and mRNA expression. Basal IL-1ra levels were not affected by treatment with any ACTH dose. In contrast, on human monocytes, β-endorphin at concentrations as low as 10 pg/ml produced an increase of basal IL-1ra protein secretion and mRNA expression, this effect being reverted by pretreatment with naloxone. No effect of β- endorphin was observed either in IL-1ra mRNA expression or protein secretion when cells were treated with LPS. The different effects of ACTH and β- endorphin could account for their differential contribution to the inflammatory response: while ACTH contributes to the glucocorticoid overall control of the inflammatory response, β-endorphin exerts an inhibitory tone on the resting IL-1 system. Because IL-1ra is essential in setting the level of monocyte and inflammatory response its differential regulation by the HPA axis hormones contributes to regulating the IL-1/inflammatory temporal response. Fil:Kovalovsky, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereda, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10217401_v6_n5_p367_Kovalovsky
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	β-Endorphin Adrenocorticotropic hormone Glucocorticoids Interleukin- 1 Interleukin-1 receptor antagonist beta endorphin corticotropin interleukin 1 receptor proopiomelanocortin article cytokine production cytokine release dose response hormonal regulation hormone action human human cell hypothalamus hypophysis system immunoregulation monocyte priority journal Adrenocorticotropic Hormone beta-Endorphin Blotting, Northern Cells, Cultured Humans Interleukin 1 Receptor Antagonist Protein Monocytes Pro-Opiomelanocortin Receptors, Interleukin-1 Sialoglycoproteins
spellingShingle	β-Endorphin Adrenocorticotropic hormone Glucocorticoids Interleukin- 1 Interleukin-1 receptor antagonist beta endorphin corticotropin interleukin 1 receptor proopiomelanocortin article cytokine production cytokine release dose response hormonal regulation hormone action human human cell hypothalamus hypophysis system immunoregulation monocyte priority journal Adrenocorticotropic Hormone beta-Endorphin Blotting, Northern Cells, Cultured Humans Interleukin 1 Receptor Antagonist Protein Monocytes Pro-Opiomelanocortin Receptors, Interleukin-1 Sialoglycoproteins Kovalovsky, D. Pereda, M.P. Stalla, G.K. Holsboer, F. Arzt, E. Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin
topic_facet	β-Endorphin Adrenocorticotropic hormone Glucocorticoids Interleukin- 1 Interleukin-1 receptor antagonist beta endorphin corticotropin interleukin 1 receptor proopiomelanocortin article cytokine production cytokine release dose response hormonal regulation hormone action human human cell hypothalamus hypophysis system immunoregulation monocyte priority journal Adrenocorticotropic Hormone beta-Endorphin Blotting, Northern Cells, Cultured Humans Interleukin 1 Receptor Antagonist Protein Monocytes Pro-Opiomelanocortin Receptors, Interleukin-1 Sialoglycoproteins
description	We have previously described the regulation of interleukin-1 receptor antagonist (IL-1ra) protein secretion and expression by IL-1, glucocorticoids and corticotropin-releasing hormone in monocytes in culture. In the present work, we analyze the direct effect of adrenocorticotropic hormone (ACTH) and β-endorphin on the expression and secretion of IL-1ra by human monocytes in culture. ACTH exerted a dose-dependent inhibitory effect on lipopolysaccharide (LPS)-induced IL-1ra production and mRNA expression. Basal IL-1ra levels were not affected by treatment with any ACTH dose. In contrast, on human monocytes, β-endorphin at concentrations as low as 10 pg/ml produced an increase of basal IL-1ra protein secretion and mRNA expression, this effect being reverted by pretreatment with naloxone. No effect of β- endorphin was observed either in IL-1ra mRNA expression or protein secretion when cells were treated with LPS. The different effects of ACTH and β- endorphin could account for their differential contribution to the inflammatory response: while ACTH contributes to the glucocorticoid overall control of the inflammatory response, β-endorphin exerts an inhibitory tone on the resting IL-1 system. Because IL-1ra is essential in setting the level of monocyte and inflammatory response its differential regulation by the HPA axis hormones contributes to regulating the IL-1/inflammatory temporal response.
format	JOUR
author	Kovalovsky, D. Pereda, M.P. Stalla, G.K. Holsboer, F. Arzt, E.
author_facet	Kovalovsky, D. Pereda, M.P. Stalla, G.K. Holsboer, F. Arzt, E.
author_sort	Kovalovsky, D.
title	Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin
title_short	Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin
title_full	Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin
title_fullStr	Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin
title_full_unstemmed	Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin
title_sort	differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin
url	http://hdl.handle.net/20.500.12110/paper_10217401_v6_n5_p367_Kovalovsky
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Differential regulation of interleukin-1 receptor antagonist by proopiomelanocortin peptides adrenocorticotropic hormone and β-endorphin

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