Development of the endosperm of Myrsine laetevirens (Myrsinaceae). I. Cellularization and deposition of cell-wall storage carbohydrates

Myrsine laetevirens has a nuclear type of endosperm development. Vacuole formation in peripheral coenocytic cytoplasm precedes cytokinesis and compartmentalizes the cytoplasm. Cellularization starts as a series of anticlinal walls projecting centripetally. Periclinal cell walls are formed from expan...

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Autores principales: Otegui, M., Lima, C., Maldonado, S., De Lederkremer, R.M.
Formato: JOUR
Materias:
Carbohydrates
Endosperm cellularization
Endosperm development
Myrsine laetevirens
Xyloglucans
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10585893_v160_n3_p491_Otegui
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_10585893_v160_n3_p491_Otegui2023-10-03T16:00:57Z Development of the endosperm of Myrsine laetevirens (Myrsinaceae). I. Cellularization and deposition of cell-wall storage carbohydrates Otegui, M. Lima, C. Maldonado, S. De Lederkremer, R.M. Carbohydrates Endosperm cellularization Endosperm development Myrsine laetevirens Xyloglucans Myrsine laetevirens Myrsine laetevirens has a nuclear type of endosperm development. Vacuole formation in peripheral coenocytic cytoplasm precedes cytokinesis and compartmentalizes the cytoplasm. Cellularization starts as a series of anticlinal walls projecting centripetally. Periclinal cell walls are formed from expanding cell plates following nuclear divisions. The developing walls are flanked by endoplasmic reticulum cisternae with dilated and short profiles and few ribosomes. Subsequent anticlinal wall growth and periclinal divisions elongate the endosperm into the central vacuole, resulting, finally, in the convergence of the advancing tissue. After that, the endosperm increases in size by anticlinal, periclinal, and oblique divisions in all cells of the endosperm and involving phragmoplast and cell plate. Carbohydrate deposition in the cell walls begins 40 d after pollination (DAP) with deposition of pectins in the cell layers next to the embryo. At ca. 50 DAP, the cell walls of the bulk endosperm begin to thicken, showing the presence of xyloglucans. The deposition of new cell-wall material is achieved by the activity of the Golgi stacks located near the cell walls. Neutral sugars were determined after two conditions of acid hydrolysis. Monosaccharides were identified by high-performance anion exchange chromatography (Dionex, Sunnyvale, Calif.). Arabinose, xylose, glucose, and galactose were the main monosaccharides, but their ratio changes during development. Methylation analysis of cell-wall polysaccharides accords with the occurrence of significant amounts of xyloglucans. The presence of pectic polysaccharides can be inferred from the amounts of uronic acids, galactose, arabinose, and rhamnose. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10585893_v160_n3_p491_Otegui
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Carbohydrates
Endosperm cellularization
Endosperm development
Myrsine laetevirens
Xyloglucans
Myrsine laetevirens
spellingShingle Carbohydrates
Endosperm cellularization
Endosperm development
Myrsine laetevirens
Xyloglucans
Myrsine laetevirens
Otegui, M.
Lima, C.
Maldonado, S.
De Lederkremer, R.M.
Development of the endosperm of Myrsine laetevirens (Myrsinaceae). I. Cellularization and deposition of cell-wall storage carbohydrates
topic_facet Carbohydrates
Endosperm cellularization
Endosperm development
Myrsine laetevirens
Xyloglucans
Myrsine laetevirens
description Myrsine laetevirens has a nuclear type of endosperm development. Vacuole formation in peripheral coenocytic cytoplasm precedes cytokinesis and compartmentalizes the cytoplasm. Cellularization starts as a series of anticlinal walls projecting centripetally. Periclinal cell walls are formed from expanding cell plates following nuclear divisions. The developing walls are flanked by endoplasmic reticulum cisternae with dilated and short profiles and few ribosomes. Subsequent anticlinal wall growth and periclinal divisions elongate the endosperm into the central vacuole, resulting, finally, in the convergence of the advancing tissue. After that, the endosperm increases in size by anticlinal, periclinal, and oblique divisions in all cells of the endosperm and involving phragmoplast and cell plate. Carbohydrate deposition in the cell walls begins 40 d after pollination (DAP) with deposition of pectins in the cell layers next to the embryo. At ca. 50 DAP, the cell walls of the bulk endosperm begin to thicken, showing the presence of xyloglucans. The deposition of new cell-wall material is achieved by the activity of the Golgi stacks located near the cell walls. Neutral sugars were determined after two conditions of acid hydrolysis. Monosaccharides were identified by high-performance anion exchange chromatography (Dionex, Sunnyvale, Calif.). Arabinose, xylose, glucose, and galactose were the main monosaccharides, but their ratio changes during development. Methylation analysis of cell-wall polysaccharides accords with the occurrence of significant amounts of xyloglucans. The presence of pectic polysaccharides can be inferred from the amounts of uronic acids, galactose, arabinose, and rhamnose.
format JOUR
author Otegui, M.
Lima, C.
Maldonado, S.
De Lederkremer, R.M.
author_facet Otegui, M.
Lima, C.
Maldonado, S.
De Lederkremer, R.M.
author_sort Otegui, M.
title Development of the endosperm of Myrsine laetevirens (Myrsinaceae). I. Cellularization and deposition of cell-wall storage carbohydrates
title_short Development of the endosperm of Myrsine laetevirens (Myrsinaceae). I. Cellularization and deposition of cell-wall storage carbohydrates
title_full Development of the endosperm of Myrsine laetevirens (Myrsinaceae). I. Cellularization and deposition of cell-wall storage carbohydrates
title_fullStr Development of the endosperm of Myrsine laetevirens (Myrsinaceae). I. Cellularization and deposition of cell-wall storage carbohydrates
title_full_unstemmed Development of the endosperm of Myrsine laetevirens (Myrsinaceae). I. Cellularization and deposition of cell-wall storage carbohydrates
title_sort development of the endosperm of myrsine laetevirens (myrsinaceae). i. cellularization and deposition of cell-wall storage carbohydrates
url http://hdl.handle.net/20.500.12110/paper_10585893_v160_n3_p491_Otegui
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