Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats

The aim of this study was to analyze whether di(2-ethylhexyl) phthalate (DEHP), a Sertoli and Leydig cell toxicant, is able to induce alterations in the expression of testicular gap and tight junction proteins. DEHP was administered by gavage (1 g/5 mL corn oil/kg body weight/ day) to 25-day-old mal...

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Autores principales:	Sobarzo, C.M., Lustig, L., Ponzio, R., Suescun, M.O., Denduchis, B.
Formato:	JOUR
Lenguaje:	English
Materias:	Claudin-11 Connexin 43 Occludin Reproductive toxicant Zonula occludens-1 claudin 11 lanthanum occludin phthalic acid bis(2 ethylhexyl) ester protein ZO1 testosterone animal experiment animal tissue article blood testis barrier body weight controlled study gap junction histopathology immunofluorescence Leydig cell male nonhuman priority journal protein expression rat Sertoli cell spermatogenesis testis testosterone blood level tight junction Western blotting Animals Connexins Diethylhexyl Phthalate Gap Junctions Leydig Cells Male Rats Rats, Sprague-Dawley Seminiferous Tubules Sertoli Cells Testis Tight Junctions Animalia Rattus Zea mays
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1059910X_v72_n11_p868_Sobarzo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_1059910X_v72_n11_p868_Sobarzo2023-10-03T16:01:05Z Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats Sobarzo, C.M. Lustig, L. Ponzio, R. Suescun, M.O. Denduchis, B. Claudin-11 Connexin 43 Occludin Reproductive toxicant Zonula occludens-1 claudin 11 lanthanum occludin phthalic acid bis(2 ethylhexyl) ester protein ZO1 testosterone animal experiment animal tissue article blood testis barrier body weight controlled study gap junction histopathology immunofluorescence Leydig cell male nonhuman priority journal protein expression rat Sertoli cell spermatogenesis testis testosterone blood level tight junction Western blotting Animals Connexins Diethylhexyl Phthalate Gap Junctions Leydig Cells Male Rats Rats, Sprague-Dawley Seminiferous Tubules Sertoli Cells Testis Tight Junctions Animalia Rattus Zea mays The aim of this study was to analyze whether di(2-ethylhexyl) phthalate (DEHP), a Sertoli and Leydig cell toxicant, is able to induce alterations in the expression of testicular gap and tight junction proteins. DEHP was administered by gavage (1 g/5 mL corn oil/kg body weight/ day) to 25-day-old male Sprague-Dawley rats for 2 days (DEHP-27d) and control rats were treated with corn-oil vehicle for 2 days (C-27d); animals were killed 24 h after the last treatment. Testes of DEHP-27d rats showed different degrees of germ cell sloughing of seminiferous tubules (ST). No alterations of the blood testis barrier (BTB) by lanthanum tracer study were observed. ST of DEHP-27d rats showed a milder immunofluorescence and more restricted expression of connexin-43 (Cx43) in the adluminal and basal compartment compared to C-27d. In DEHP-27d rats, we found a discontinuous immunofluorescent (IF) pattern for zonula occludens (ZO-1), contrasting with the continuous IF profile observed in C-27d, and a delocalization of claudin-11. A decrease in Cx43 and ZO-1 and no changes in occludin expression were detected by Western blot in the testes of DEHP-27d rats. Results from 57-day-old rats treated with DEHP for 2 days and held for 30 days without treatment showed that the alterations in protein expression induced by DEHP are reversible. However, a delay of spermatogenesis compared to C-57d rats, occurred. Data demonstrated that DEHP does not impair BTB permeability but induces germ cell sloughing that might respond to a down regulation of Cx43 and ZO-1 that alters cell junction proteins. © 2009 Wiley-Liss, Inc. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1059910X_v72_n11_p868_Sobarzo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	English
orig_language_str_mv	English
topic	Claudin-11 Connexin 43 Occludin Reproductive toxicant Zonula occludens-1 claudin 11 lanthanum occludin phthalic acid bis(2 ethylhexyl) ester protein ZO1 testosterone animal experiment animal tissue article blood testis barrier body weight controlled study gap junction histopathology immunofluorescence Leydig cell male nonhuman priority journal protein expression rat Sertoli cell spermatogenesis testis testosterone blood level tight junction Western blotting Animals Connexins Diethylhexyl Phthalate Gap Junctions Leydig Cells Male Rats Rats, Sprague-Dawley Seminiferous Tubules Sertoli Cells Testis Tight Junctions Animalia Rattus Zea mays
spellingShingle	Claudin-11 Connexin 43 Occludin Reproductive toxicant Zonula occludens-1 claudin 11 lanthanum occludin phthalic acid bis(2 ethylhexyl) ester protein ZO1 testosterone animal experiment animal tissue article blood testis barrier body weight controlled study gap junction histopathology immunofluorescence Leydig cell male nonhuman priority journal protein expression rat Sertoli cell spermatogenesis testis testosterone blood level tight junction Western blotting Animals Connexins Diethylhexyl Phthalate Gap Junctions Leydig Cells Male Rats Rats, Sprague-Dawley Seminiferous Tubules Sertoli Cells Testis Tight Junctions Animalia Rattus Zea mays Sobarzo, C.M. Lustig, L. Ponzio, R. Suescun, M.O. Denduchis, B. Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats
topic_facet	Claudin-11 Connexin 43 Occludin Reproductive toxicant Zonula occludens-1 claudin 11 lanthanum occludin phthalic acid bis(2 ethylhexyl) ester protein ZO1 testosterone animal experiment animal tissue article blood testis barrier body weight controlled study gap junction histopathology immunofluorescence Leydig cell male nonhuman priority journal protein expression rat Sertoli cell spermatogenesis testis testosterone blood level tight junction Western blotting Animals Connexins Diethylhexyl Phthalate Gap Junctions Leydig Cells Male Rats Rats, Sprague-Dawley Seminiferous Tubules Sertoli Cells Testis Tight Junctions Animalia Rattus Zea mays
description	The aim of this study was to analyze whether di(2-ethylhexyl) phthalate (DEHP), a Sertoli and Leydig cell toxicant, is able to induce alterations in the expression of testicular gap and tight junction proteins. DEHP was administered by gavage (1 g/5 mL corn oil/kg body weight/ day) to 25-day-old male Sprague-Dawley rats for 2 days (DEHP-27d) and control rats were treated with corn-oil vehicle for 2 days (C-27d); animals were killed 24 h after the last treatment. Testes of DEHP-27d rats showed different degrees of germ cell sloughing of seminiferous tubules (ST). No alterations of the blood testis barrier (BTB) by lanthanum tracer study were observed. ST of DEHP-27d rats showed a milder immunofluorescence and more restricted expression of connexin-43 (Cx43) in the adluminal and basal compartment compared to C-27d. In DEHP-27d rats, we found a discontinuous immunofluorescent (IF) pattern for zonula occludens (ZO-1), contrasting with the continuous IF profile observed in C-27d, and a delocalization of claudin-11. A decrease in Cx43 and ZO-1 and no changes in occludin expression were detected by Western blot in the testes of DEHP-27d rats. Results from 57-day-old rats treated with DEHP for 2 days and held for 30 days without treatment showed that the alterations in protein expression induced by DEHP are reversible. However, a delay of spermatogenesis compared to C-57d rats, occurred. Data demonstrated that DEHP does not impair BTB permeability but induces germ cell sloughing that might respond to a down regulation of Cx43 and ZO-1 that alters cell junction proteins. © 2009 Wiley-Liss, Inc.
format	JOUR
author	Sobarzo, C.M. Lustig, L. Ponzio, R. Suescun, M.O. Denduchis, B.
author_facet	Sobarzo, C.M. Lustig, L. Ponzio, R. Suescun, M.O. Denduchis, B.
author_sort	Sobarzo, C.M.
title	Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats
title_short	Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats
title_full	Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats
title_fullStr	Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats
title_full_unstemmed	Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats
title_sort	effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats
url	http://hdl.handle.net/20.500.12110/paper_1059910X_v72_n11_p868_Sobarzo
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Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats

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