Characterisation of fiber obtained from pumpkin (cucumis moschata duch.) mesocarp through enzymatic treatment

Cell wall-enriched pumpkin (Cucumis moschata Duch.) powder was submitted to enzymatic hydrolysis by cellulase or hemicellulase in order to evaluate the performance of these cell wall-degrading enzymes on that substrate. Different enzyme-substrate ratios were evaluated and the effect exerted by the b...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Fissore, E.N., Ponce, N.M., Stortz, C.A., Rojas, A.M., Gerschenson, L.N.
Formato: JOUR
Materias:
Enzymes
Industrial wastes
Pectin
Pumpkin
Cells
Enzyme kinetics
Fruits
Hydrolysis
Macromolecules
pH effects
Polysaccharides
Acidic citrate buffers
Enzymatic treatment
Fibers
Cucumis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10820132_v13_n2_p141_Fissore
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_10820132_v13_n2_p141_Fissore
record_format dspace
spelling todo:paper_10820132_v13_n2_p141_Fissore2023-10-03T16:03:53Z Characterisation of fiber obtained from pumpkin (cucumis moschata duch.) mesocarp through enzymatic treatment Fissore, E.N. Ponce, N.M. Stortz, C.A. Rojas, A.M. Gerschenson, L.N. Enzymes Industrial wastes Pectin Pumpkin Cells Enzyme kinetics Fruits Hydrolysis Industrial wastes Macromolecules pH effects Polysaccharides Acidic citrate buffers Enzymatic treatment Pectin Pumpkin Fibers Cucumis Cell wall-enriched pumpkin (Cucumis moschata Duch.) powder was submitted to enzymatic hydrolysis by cellulase or hemicellulase in order to evaluate the performance of these cell wall-degrading enzymes on that substrate. Different enzyme-substrate ratios were evaluated and the effect exerted by the buffer on cell wall polysaccharides. Cellulase produced the release of pectin macromolecules which include homogalacturonans side chains, the rhamnogalacturonan I core and rhamnogalacturonan II, in conjunction with xylogalacturonans. The content of galacturonic acid in product obtained ranged from 545 to 781 g/kg of fiber. Hemicellulases produced intense pectin hydrolysis leading to fiber-fractions with galacturonic acid contents ranging from 390 to 444 g/kg of fiber and enriched in glucose polymers as the enzyme proportion increased. Few rhamnogalacturonan-I was present.The acidic citrate buffer (pH 5.2) used for allowing enzyme activity could per se remove noncovalent cross-links like ionic bonds. As a consequence, pectin-in-extensin entanglements, pectins joined by Ca2+-bridges through the homogalacturonan side chains, and some pectins that are originally soluble in cold water due to little or no binding to the cell wall, could be removed by this citrate buffer. Enzymatic hydrolysis as well as buffer extraction produced fiber-products with an important thickening effect of aqueous systems. This effect was smaller as the ratio enzyme-substrate was increased and, in general, the fiber fractions isolated produced an in vitro glucose diffusion retardation. © 2007 SAGE Publications. Fil:Fissore, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rojas, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gerschenson, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10820132_v13_n2_p141_Fissore
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Enzymes
Industrial wastes
Pectin
Pumpkin
Cells
Enzyme kinetics
Fruits
Hydrolysis
Industrial wastes
Macromolecules
pH effects
Polysaccharides
Acidic citrate buffers
Enzymatic treatment
Pectin
Pumpkin
Fibers
Cucumis
spellingShingle Enzymes
Industrial wastes
Pectin
Pumpkin
Cells
Enzyme kinetics
Fruits
Hydrolysis
Industrial wastes
Macromolecules
pH effects
Polysaccharides
Acidic citrate buffers
Enzymatic treatment
Pectin
Pumpkin
Fibers
Cucumis
Fissore, E.N.
Ponce, N.M.
Stortz, C.A.
Rojas, A.M.
Gerschenson, L.N.
Characterisation of fiber obtained from pumpkin (cucumis moschata duch.) mesocarp through enzymatic treatment
topic_facet Enzymes
Industrial wastes
Pectin
Pumpkin
Cells
Enzyme kinetics
Fruits
Hydrolysis
Industrial wastes
Macromolecules
pH effects
Polysaccharides
Acidic citrate buffers
Enzymatic treatment
Pectin
Pumpkin
Fibers
Cucumis
description Cell wall-enriched pumpkin (Cucumis moschata Duch.) powder was submitted to enzymatic hydrolysis by cellulase or hemicellulase in order to evaluate the performance of these cell wall-degrading enzymes on that substrate. Different enzyme-substrate ratios were evaluated and the effect exerted by the buffer on cell wall polysaccharides. Cellulase produced the release of pectin macromolecules which include homogalacturonans side chains, the rhamnogalacturonan I core and rhamnogalacturonan II, in conjunction with xylogalacturonans. The content of galacturonic acid in product obtained ranged from 545 to 781 g/kg of fiber. Hemicellulases produced intense pectin hydrolysis leading to fiber-fractions with galacturonic acid contents ranging from 390 to 444 g/kg of fiber and enriched in glucose polymers as the enzyme proportion increased. Few rhamnogalacturonan-I was present.The acidic citrate buffer (pH 5.2) used for allowing enzyme activity could per se remove noncovalent cross-links like ionic bonds. As a consequence, pectin-in-extensin entanglements, pectins joined by Ca2+-bridges through the homogalacturonan side chains, and some pectins that are originally soluble in cold water due to little or no binding to the cell wall, could be removed by this citrate buffer. Enzymatic hydrolysis as well as buffer extraction produced fiber-products with an important thickening effect of aqueous systems. This effect was smaller as the ratio enzyme-substrate was increased and, in general, the fiber fractions isolated produced an in vitro glucose diffusion retardation. © 2007 SAGE Publications.
format JOUR
author Fissore, E.N.
Ponce, N.M.
Stortz, C.A.
Rojas, A.M.
Gerschenson, L.N.
author_facet Fissore, E.N.
Ponce, N.M.
Stortz, C.A.
Rojas, A.M.
Gerschenson, L.N.
author_sort Fissore, E.N.
title Characterisation of fiber obtained from pumpkin (cucumis moschata duch.) mesocarp through enzymatic treatment
title_short Characterisation of fiber obtained from pumpkin (cucumis moschata duch.) mesocarp through enzymatic treatment
title_full Characterisation of fiber obtained from pumpkin (cucumis moschata duch.) mesocarp through enzymatic treatment
title_fullStr Characterisation of fiber obtained from pumpkin (cucumis moschata duch.) mesocarp through enzymatic treatment
title_full_unstemmed Characterisation of fiber obtained from pumpkin (cucumis moschata duch.) mesocarp through enzymatic treatment
title_sort characterisation of fiber obtained from pumpkin (cucumis moschata duch.) mesocarp through enzymatic treatment
url http://hdl.handle.net/20.500.12110/paper_10820132_v13_n2_p141_Fissore
work_keys_str_mv AT fissoreen characterisationoffiberobtainedfrompumpkincucumismoschataduchmesocarpthroughenzymatictreatment
AT poncenm characterisationoffiberobtainedfrompumpkincucumismoschataduchmesocarpthroughenzymatictreatment
AT stortzca characterisationoffiberobtainedfrompumpkincucumismoschataduchmesocarpthroughenzymatictreatment
AT rojasam characterisationoffiberobtainedfrompumpkincucumismoschataduchmesocarpthroughenzymatictreatment
AT gerschensonln characterisationoffiberobtainedfrompumpkincucumismoschataduchmesocarpthroughenzymatictreatment
_version_ 1807317071615229952

Ejemplares similares