An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives
A simple reversed phase HPLC method was developed for the determination of polyamines (putrescine, cadaverine, spermidine, spermine) and other polycations (n-acetyl putrescine, histamine, n-acetilspermine, agmatine), as their benzoylated derivatives. The advantages of this method, examined in variou...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10826076_v23_n5_p693_Hockl |
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todo:paper_10826076_v23_n5_p693_Hockl2023-10-03T16:03:59Z An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives Hockl, P.F. Thyssen, S.M. Libertun, C. agmatine cadaverine histamine polyamine polycation putrescine putrescine derivative spermidine spermine spermine derivative animal tissue article calibration chromatography derivatization female high performance liquid chromatography hypophysis hypothalamus nonhuman ovary polyamine synthesis rat reproducibility reversed phase high performance liquid chromatography seminal plasma standard A simple reversed phase HPLC method was developed for the determination of polyamines (putrescine, cadaverine, spermidine, spermine) and other polycations (n-acetyl putrescine, histamine, n-acetilspermine, agmatine), as their benzoylated derivatives. The advantages of this method, examined in various matrixes (hypothalamus, hypophyses, ovary, and seminal plasma), are its versatility, simplicity, and short time of determination of polyamines and related substances of importance in prokaryotes and eukaryotes, even in different matrixes, using a standard HPLC equipment (UV detection at 254 nm), with good resolution, sensitivity, and reproducibility. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10826076_v23_n5_p693_Hockl |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
agmatine cadaverine histamine polyamine polycation putrescine putrescine derivative spermidine spermine spermine derivative animal tissue article calibration chromatography derivatization female high performance liquid chromatography hypophysis hypothalamus nonhuman ovary polyamine synthesis rat reproducibility reversed phase high performance liquid chromatography seminal plasma standard |
| spellingShingle |
agmatine cadaverine histamine polyamine polycation putrescine putrescine derivative spermidine spermine spermine derivative animal tissue article calibration chromatography derivatization female high performance liquid chromatography hypophysis hypothalamus nonhuman ovary polyamine synthesis rat reproducibility reversed phase high performance liquid chromatography seminal plasma standard Hockl, P.F. Thyssen, S.M. Libertun, C. An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives |
| topic_facet |
agmatine cadaverine histamine polyamine polycation putrescine putrescine derivative spermidine spermine spermine derivative animal tissue article calibration chromatography derivatization female high performance liquid chromatography hypophysis hypothalamus nonhuman ovary polyamine synthesis rat reproducibility reversed phase high performance liquid chromatography seminal plasma standard |
| description |
A simple reversed phase HPLC method was developed for the determination of polyamines (putrescine, cadaverine, spermidine, spermine) and other polycations (n-acetyl putrescine, histamine, n-acetilspermine, agmatine), as their benzoylated derivatives. The advantages of this method, examined in various matrixes (hypothalamus, hypophyses, ovary, and seminal plasma), are its versatility, simplicity, and short time of determination of polyamines and related substances of importance in prokaryotes and eukaryotes, even in different matrixes, using a standard HPLC equipment (UV detection at 254 nm), with good resolution, sensitivity, and reproducibility. |
| format |
JOUR |
| author |
Hockl, P.F. Thyssen, S.M. Libertun, C. |
| author_facet |
Hockl, P.F. Thyssen, S.M. Libertun, C. |
| author_sort |
Hockl, P.F. |
| title |
An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives |
| title_short |
An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives |
| title_full |
An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives |
| title_fullStr |
An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives |
| title_full_unstemmed |
An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives |
| title_sort |
improved hplc method for identification and quantitation of polyamines and related compounds as benzoylated derivatives |
| url |
http://hdl.handle.net/20.500.12110/paper_10826076_v23_n5_p693_Hockl |
| work_keys_str_mv |
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