Main features of the oxidative metabolism in gills and liver of Odontesthes nigricans Richardson (Pisces, Atherinopsidae)
The aim of this work was to study comparatively the oxidative metabolism in gills and liver of a silverside, Odontesthes nigricans, in their natural environment, the Beagle Channel. Oxidative damage to lipids was evaluated by assessing TBARS and lipid radical content, in gills and liver. Gills showe...
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todo:paper_10964959_v154_n4_p406_Lattuca2023-10-03T16:05:23Z Main features of the oxidative metabolism in gills and liver of Odontesthes nigricans Richardson (Pisces, Atherinopsidae) Lattuca, M.E. Malanga, G. Hurtado, C.A. Pérez, A.F. Calvo, J. Puntarulo, S. Antioxidants Beagle Channel Lipid peroxidation Odontesthes nigricans Oxidative metabolism alpha tocopherol ascorbic acid beta carotene catalase lipid radical thiobarbituric acid reactive substance aerobic metabolism animal experiment animal model article cell damage controlled study environmental exposure enzyme activity fish disease gill lipid oxidation liver metabolism nonhuman oxidative stress priority journal Animals Antioxidants Electron Spin Resonance Spectroscopy Female Fishes Free Radicals Gills Intracellular Space Lipid Metabolism Lipid Peroxidation Liver Male Oxidative Stress Reactive Oxygen Species Water Argentina elongata Atherinopsidae Odontesthes nigricans Pisces The aim of this work was to study comparatively the oxidative metabolism in gills and liver of a silverside, Odontesthes nigricans, in their natural environment, the Beagle Channel. Oxidative damage to lipids was evaluated by assessing TBARS and lipid radical content, in gills and liver. Gills showed a significantly higher degree of damage than liver. The content of α-tocopherol, β-carotene and catalase activity showed significantly higher values in the liver than in the gills. The ascorbyl radical (A•) content showed no significant differences between gills and liver. The ascorbate (AH-) content was 12 ± 2 and 159 ± 28 nmol/mg FW in gills and liver, respectively. Oxidative metabolism at the hydrophilic level was assessed as the ratio A•/AH-. The ratio A•/AH- was significantly different between organs, (6 ± 2)10- 5 and (5 ± 2)10- 6, for the gills and the liver, respectively. Both, lipid radical content/α-tocopherol content and lipid radical content/β-carotene content ratios were significantly higher in gills as compared to the values recorded for the liver, suggesting an increased situation of oxidative stress condition in the lipid phase of the gills. Taken as a whole, the O. nigricans liver exhibited a better control of oxidative damage than the gills, allowing minimization of intracellular damage when exposed to environmental stressing conditions. © 2009 Elsevier Inc. All rights reserved. Fil:Lattuca, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10964959_v154_n4_p406_Lattuca |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antioxidants Beagle Channel Lipid peroxidation Odontesthes nigricans Oxidative metabolism alpha tocopherol ascorbic acid beta carotene catalase lipid radical thiobarbituric acid reactive substance aerobic metabolism animal experiment animal model article cell damage controlled study environmental exposure enzyme activity fish disease gill lipid oxidation liver metabolism nonhuman oxidative stress priority journal Animals Antioxidants Electron Spin Resonance Spectroscopy Female Fishes Free Radicals Gills Intracellular Space Lipid Metabolism Lipid Peroxidation Liver Male Oxidative Stress Reactive Oxygen Species Water Argentina elongata Atherinopsidae Odontesthes nigricans Pisces |
| spellingShingle |
Antioxidants Beagle Channel Lipid peroxidation Odontesthes nigricans Oxidative metabolism alpha tocopherol ascorbic acid beta carotene catalase lipid radical thiobarbituric acid reactive substance aerobic metabolism animal experiment animal model article cell damage controlled study environmental exposure enzyme activity fish disease gill lipid oxidation liver metabolism nonhuman oxidative stress priority journal Animals Antioxidants Electron Spin Resonance Spectroscopy Female Fishes Free Radicals Gills Intracellular Space Lipid Metabolism Lipid Peroxidation Liver Male Oxidative Stress Reactive Oxygen Species Water Argentina elongata Atherinopsidae Odontesthes nigricans Pisces Lattuca, M.E. Malanga, G. Hurtado, C.A. Pérez, A.F. Calvo, J. Puntarulo, S. Main features of the oxidative metabolism in gills and liver of Odontesthes nigricans Richardson (Pisces, Atherinopsidae) |
| topic_facet |
Antioxidants Beagle Channel Lipid peroxidation Odontesthes nigricans Oxidative metabolism alpha tocopherol ascorbic acid beta carotene catalase lipid radical thiobarbituric acid reactive substance aerobic metabolism animal experiment animal model article cell damage controlled study environmental exposure enzyme activity fish disease gill lipid oxidation liver metabolism nonhuman oxidative stress priority journal Animals Antioxidants Electron Spin Resonance Spectroscopy Female Fishes Free Radicals Gills Intracellular Space Lipid Metabolism Lipid Peroxidation Liver Male Oxidative Stress Reactive Oxygen Species Water Argentina elongata Atherinopsidae Odontesthes nigricans Pisces |
| description |
The aim of this work was to study comparatively the oxidative metabolism in gills and liver of a silverside, Odontesthes nigricans, in their natural environment, the Beagle Channel. Oxidative damage to lipids was evaluated by assessing TBARS and lipid radical content, in gills and liver. Gills showed a significantly higher degree of damage than liver. The content of α-tocopherol, β-carotene and catalase activity showed significantly higher values in the liver than in the gills. The ascorbyl radical (A•) content showed no significant differences between gills and liver. The ascorbate (AH-) content was 12 ± 2 and 159 ± 28 nmol/mg FW in gills and liver, respectively. Oxidative metabolism at the hydrophilic level was assessed as the ratio A•/AH-. The ratio A•/AH- was significantly different between organs, (6 ± 2)10- 5 and (5 ± 2)10- 6, for the gills and the liver, respectively. Both, lipid radical content/α-tocopherol content and lipid radical content/β-carotene content ratios were significantly higher in gills as compared to the values recorded for the liver, suggesting an increased situation of oxidative stress condition in the lipid phase of the gills. Taken as a whole, the O. nigricans liver exhibited a better control of oxidative damage than the gills, allowing minimization of intracellular damage when exposed to environmental stressing conditions. © 2009 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Lattuca, M.E. Malanga, G. Hurtado, C.A. Pérez, A.F. Calvo, J. Puntarulo, S. |
| author_facet |
Lattuca, M.E. Malanga, G. Hurtado, C.A. Pérez, A.F. Calvo, J. Puntarulo, S. |
| author_sort |
Lattuca, M.E. |
| title |
Main features of the oxidative metabolism in gills and liver of Odontesthes nigricans Richardson (Pisces, Atherinopsidae) |
| title_short |
Main features of the oxidative metabolism in gills and liver of Odontesthes nigricans Richardson (Pisces, Atherinopsidae) |
| title_full |
Main features of the oxidative metabolism in gills and liver of Odontesthes nigricans Richardson (Pisces, Atherinopsidae) |
| title_fullStr |
Main features of the oxidative metabolism in gills and liver of Odontesthes nigricans Richardson (Pisces, Atherinopsidae) |
| title_full_unstemmed |
Main features of the oxidative metabolism in gills and liver of Odontesthes nigricans Richardson (Pisces, Atherinopsidae) |
| title_sort |
main features of the oxidative metabolism in gills and liver of odontesthes nigricans richardson (pisces, atherinopsidae) |
| url |
http://hdl.handle.net/20.500.12110/paper_10964959_v154_n4_p406_Lattuca |
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