Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent

Diachasmimorpha longicaudata (hymenoptera: braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. in this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. the cytogenetic ana...

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Autores principales: Carabajal Paladino, L., Papeschi, A., Lanzavecchia, S., Cladera, J., Bressa, M.J.
Formato: JOUR
Materias:
Ag-NOR
Braconidae
C-banding
Diachasmimorpha longicaudata
FISH
Heterochromatin
Hymenoptera
Karyotype
rDNA
biological control
chromosome
cytogenetics
DNA
fly
genomics
karyotype
parasitoid
wasp
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_12105759_v110_n3_p401_CarabajalPaladino
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_12105759_v110_n3_p401_CarabajalPaladino
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spelling todo:paper_12105759_v110_n3_p401_CarabajalPaladino2023-10-03T16:09:01Z Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent Carabajal Paladino, L. Papeschi, A. Lanzavecchia, S. Cladera, J. Bressa, M.J. Ag-NOR Braconidae C-banding Diachasmimorpha longicaudata FISH Heterochromatin Hymenoptera Karyotype rDNA biological control chromosome cytogenetics DNA fly genomics karyotype parasitoid wasp Diachasmimorpha longicaudata (hymenoptera: braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. in this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. the cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). the entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. the high heterochromatin content differentiates d. longicaudata from other braconid species. fluorescence in situ hybridization (fish) using autologous 18s rdna probes revealed six clusters of rdna, i.e. six nucleolar organizer regions (nors), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. this number is exceptionally high for hymenoptera, which usually have two nors in the diploid complement. it is noteworthy that these rdna-fish experiments represent the first use of this technique on a braconid species using autologous probes. since ag-nor-bands were coincident with c-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. the physical characteristics of the d. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rdna clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies. Fil:Carabajal Paladino, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Papeschi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanzavecchia, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cladera, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bressa, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_12105759_v110_n3_p401_CarabajalPaladino
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ag-NOR
Braconidae
C-banding
Diachasmimorpha longicaudata
FISH
Heterochromatin
Hymenoptera
Karyotype
rDNA
biological control
chromosome
cytogenetics
DNA
fly
genomics
karyotype
parasitoid
wasp
spellingShingle Ag-NOR
Braconidae
C-banding
Diachasmimorpha longicaudata
FISH
Heterochromatin
Hymenoptera
Karyotype
rDNA
biological control
chromosome
cytogenetics
DNA
fly
genomics
karyotype
parasitoid
wasp
Carabajal Paladino, L.
Papeschi, A.
Lanzavecchia, S.
Cladera, J.
Bressa, M.J.
Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
topic_facet Ag-NOR
Braconidae
C-banding
Diachasmimorpha longicaudata
FISH
Heterochromatin
Hymenoptera
Karyotype
rDNA
biological control
chromosome
cytogenetics
DNA
fly
genomics
karyotype
parasitoid
wasp
description Diachasmimorpha longicaudata (hymenoptera: braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. in this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. the cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). the entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. the high heterochromatin content differentiates d. longicaudata from other braconid species. fluorescence in situ hybridization (fish) using autologous 18s rdna probes revealed six clusters of rdna, i.e. six nucleolar organizer regions (nors), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. this number is exceptionally high for hymenoptera, which usually have two nors in the diploid complement. it is noteworthy that these rdna-fish experiments represent the first use of this technique on a braconid species using autologous probes. since ag-nor-bands were coincident with c-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. the physical characteristics of the d. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rdna clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies.
format JOUR
author Carabajal Paladino, L.
Papeschi, A.
Lanzavecchia, S.
Cladera, J.
Bressa, M.J.
author_facet Carabajal Paladino, L.
Papeschi, A.
Lanzavecchia, S.
Cladera, J.
Bressa, M.J.
author_sort Carabajal Paladino, L.
title Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
title_short Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
title_full Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
title_fullStr Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
title_full_unstemmed Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
title_sort cytogenetic characterization of diachasmimorpha longicaudata (hymenoptera: braconidae), a parasitoid wasp used as a biological control agent
url http://hdl.handle.net/20.500.12110/paper_12105759_v110_n3_p401_CarabajalPaladino
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