N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes

Objectives: Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat...

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Autores principales:	Calzadilla, P., Gómez-Serrano, M., García-Santos, E., Schiappacasse, A., Abalde, Y., Calvo, J.C., Peral, B., Guerra, L.N.
Formato:	JOUR
Materias:	Adipose differentiation N-acetylcysteine Obesity proteins acetylcysteine amine oxidase (flavin containing) isoenzyme A aminoacylase fatty acid binding protein 4 heat shock protein 70 transketolase triacylglycerol adipogenesis amino acid metabolism animal cell article carbohydrate metabolism cell differentiation cell maturation cell proliferation cell strain 3T3 cell viability concentration response controlled study drug effect drug mechanism lipid storage lipogenesis mouse nonhuman obesity oxidative stress priority journal proadipocyte protein expression 3T3-L1 Cells Acetylcysteine Adipocytes Adipogenesis Amidohydrolases Animals Cell Differentiation Fatty Acid-Binding Proteins HSP72 Heat-Shock Proteins Mice Monoamine Oxidase Oxidative Stress Transketolase Triglycerides
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13510002_v18_n6_p210_Calzadilla
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_13510002_v18_n6_p210_Calzadilla
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spelling	todo:paper_13510002_v18_n6_p210_Calzadilla2023-10-03T16:10:05Z N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes Calzadilla, P. Gómez-Serrano, M. García-Santos, E. Schiappacasse, A. Abalde, Y. Calvo, J.C. Peral, B. Guerra, L.N. Adipose differentiation N-acetylcysteine Obesity proteins acetylcysteine amine oxidase (flavin containing) isoenzyme A aminoacylase fatty acid binding protein 4 heat shock protein 70 transketolase triacylglycerol adipogenesis amino acid metabolism animal cell article carbohydrate metabolism cell differentiation cell maturation cell proliferation cell strain 3T3 cell viability concentration response controlled study drug effect drug mechanism lipid storage lipogenesis mouse nonhuman obesity oxidative stress priority journal proadipocyte protein expression 3T3-L1 Cells Acetylcysteine Adipocytes Adipogenesis Amidohydrolases Animals Cell Differentiation Fatty Acid-Binding Proteins HSP72 Heat-Shock Proteins Mice Monoamine Oxidase Oxidative Stress Transketolase Triglycerides Objectives: Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat accumulation and the expression of obesity-associated proteins. Methods: We studied the effects of 10 μM NAC on obesity-related protein expression in cultured 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids. Results: NAC treatment inhibited fat accumulation and reduced the expression of obesity-related proteins, including monoamine oxidase A, heat shock protein 70 (HSP70), aminoacylase -1 (ACY-1), and transketolase. Discussion: Our results suggest that the effects of NAC on triglycerides (Tgs) and protein expression are correlated. In support of this, we showed that NAC treatment affected both the Tg synthesis pathway and the expression levels of proteins implicated in human obesity. © W. S. Maney & Son Ltd 2013. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13510002_v18_n6_p210_Calzadilla
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adipose differentiation N-acetylcysteine Obesity proteins acetylcysteine amine oxidase (flavin containing) isoenzyme A aminoacylase fatty acid binding protein 4 heat shock protein 70 transketolase triacylglycerol adipogenesis amino acid metabolism animal cell article carbohydrate metabolism cell differentiation cell maturation cell proliferation cell strain 3T3 cell viability concentration response controlled study drug effect drug mechanism lipid storage lipogenesis mouse nonhuman obesity oxidative stress priority journal proadipocyte protein expression 3T3-L1 Cells Acetylcysteine Adipocytes Adipogenesis Amidohydrolases Animals Cell Differentiation Fatty Acid-Binding Proteins HSP72 Heat-Shock Proteins Mice Monoamine Oxidase Oxidative Stress Transketolase Triglycerides
spellingShingle	Adipose differentiation N-acetylcysteine Obesity proteins acetylcysteine amine oxidase (flavin containing) isoenzyme A aminoacylase fatty acid binding protein 4 heat shock protein 70 transketolase triacylglycerol adipogenesis amino acid metabolism animal cell article carbohydrate metabolism cell differentiation cell maturation cell proliferation cell strain 3T3 cell viability concentration response controlled study drug effect drug mechanism lipid storage lipogenesis mouse nonhuman obesity oxidative stress priority journal proadipocyte protein expression 3T3-L1 Cells Acetylcysteine Adipocytes Adipogenesis Amidohydrolases Animals Cell Differentiation Fatty Acid-Binding Proteins HSP72 Heat-Shock Proteins Mice Monoamine Oxidase Oxidative Stress Transketolase Triglycerides Calzadilla, P. Gómez-Serrano, M. García-Santos, E. Schiappacasse, A. Abalde, Y. Calvo, J.C. Peral, B. Guerra, L.N. N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
topic_facet	Adipose differentiation N-acetylcysteine Obesity proteins acetylcysteine amine oxidase (flavin containing) isoenzyme A aminoacylase fatty acid binding protein 4 heat shock protein 70 transketolase triacylglycerol adipogenesis amino acid metabolism animal cell article carbohydrate metabolism cell differentiation cell maturation cell proliferation cell strain 3T3 cell viability concentration response controlled study drug effect drug mechanism lipid storage lipogenesis mouse nonhuman obesity oxidative stress priority journal proadipocyte protein expression 3T3-L1 Cells Acetylcysteine Adipocytes Adipogenesis Amidohydrolases Animals Cell Differentiation Fatty Acid-Binding Proteins HSP72 Heat-Shock Proteins Mice Monoamine Oxidase Oxidative Stress Transketolase Triglycerides
description	Objectives: Oxidative stress plays critical roles in the pathogeneses of diabetes, hypertension, and atherosclerosis, but its effect on fat accumulation is still unclear. In this study, we analyzed the role of the well-known antioxidant and a glutathione (GSH) precursor N-acetylcysteine (NAC) in fat accumulation and the expression of obesity-associated proteins. Methods: We studied the effects of 10 μM NAC on obesity-related protein expression in cultured 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids. Results: NAC treatment inhibited fat accumulation and reduced the expression of obesity-related proteins, including monoamine oxidase A, heat shock protein 70 (HSP70), aminoacylase -1 (ACY-1), and transketolase. Discussion: Our results suggest that the effects of NAC on triglycerides (Tgs) and protein expression are correlated. In support of this, we showed that NAC treatment affected both the Tg synthesis pathway and the expression levels of proteins implicated in human obesity. © W. S. Maney & Son Ltd 2013.
format	JOUR
author	Calzadilla, P. Gómez-Serrano, M. García-Santos, E. Schiappacasse, A. Abalde, Y. Calvo, J.C. Peral, B. Guerra, L.N.
author_facet	Calzadilla, P. Gómez-Serrano, M. García-Santos, E. Schiappacasse, A. Abalde, Y. Calvo, J.C. Peral, B. Guerra, L.N.
author_sort	Calzadilla, P.
title	N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
title_short	N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
title_full	N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
title_fullStr	N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
title_full_unstemmed	N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes
title_sort	n-acetylcysteine affects obesity-related protein expression in 3t3-l1 adipocytes
url	http://hdl.handle.net/20.500.12110/paper_13510002_v18_n6_p210_Calzadilla
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N-Acetylcysteine affects obesity-related protein expression in 3T3-L1 adipocytes

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