Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae
It has been previously reported that 5-aminolevulinic acid (ALA) and 4-aminobutyric acid (GABA) share a common permease in Saccharomyces cerevisiae (Bermúdez Moretti et al., 1993). The aim of the present work was to determine the relationship between the transport of these compounds in isolated cell...
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todo:paper_13572725_v27_n2_p169_BermudezMoretti2023-10-03T16:10:25Z Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae Bermúdez Moretti, M. Correa García, S.R. Chianelli, M.S. Ramos, E.H. Mattoon, J.R. Batlle, A. 4-Aminobutyric acid 5-Aminolevulinic acid Membrane transport Permease Saccharomyces cerevisiae 4 aminobutyric acid aminolevulinic acid amino acid transport article isotope labeling saccharomyces cerevisiae Aminolevulinic Acid Biological Transport Cell Membrane Culture Media gamma-Aminobutyric Acid Kinetics Membrane Transport Proteins Saccharomyces cerevisiae Support, Non-U.S. Gov't Time Factors It has been previously reported that 5-aminolevulinic acid (ALA) and 4-aminobutyric acid (GABA) share a common permease in Saccharomyces cerevisiae (Bermúdez Moretti et al., 1993). The aim of the present work was to determine the relationship between the transport of these compounds in isolated cells. Assessment of amino acid incorporation was performed in S. cerevisiae using 14C-ALA or 3H-GABA. Initial rates of ALA incorporation in cells grown in the presence of 5 mM ALA and 5 mM GABA, were three to four times lower than in cells grown without supplements. Kinetic studies indicate that GABA competitively inhibits ALA transport. During the growth phase GABA uptake was also inhibited by 74% and 60% in the presence of ALA and GABA, respectively. These findings indicate that in S. cerevisiae the structurally related compounds, ALA and GABA, may be incorporated into the cells by a common carrier protein. Should this occur in other lukaryotic cells it may explain the neurotoxic effect attributed to ALA in the pathogenesis of acute porphyrias. © 1995. Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Correa García, S.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Chianelli, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramos, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13572725_v27_n2_p169_BermudezMoretti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
4-Aminobutyric acid 5-Aminolevulinic acid Membrane transport Permease Saccharomyces cerevisiae 4 aminobutyric acid aminolevulinic acid amino acid transport article isotope labeling saccharomyces cerevisiae Aminolevulinic Acid Biological Transport Cell Membrane Culture Media gamma-Aminobutyric Acid Kinetics Membrane Transport Proteins Saccharomyces cerevisiae Support, Non-U.S. Gov't Time Factors |
| spellingShingle |
4-Aminobutyric acid 5-Aminolevulinic acid Membrane transport Permease Saccharomyces cerevisiae 4 aminobutyric acid aminolevulinic acid amino acid transport article isotope labeling saccharomyces cerevisiae Aminolevulinic Acid Biological Transport Cell Membrane Culture Media gamma-Aminobutyric Acid Kinetics Membrane Transport Proteins Saccharomyces cerevisiae Support, Non-U.S. Gov't Time Factors Bermúdez Moretti, M. Correa García, S.R. Chianelli, M.S. Ramos, E.H. Mattoon, J.R. Batlle, A. Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae |
| topic_facet |
4-Aminobutyric acid 5-Aminolevulinic acid Membrane transport Permease Saccharomyces cerevisiae 4 aminobutyric acid aminolevulinic acid amino acid transport article isotope labeling saccharomyces cerevisiae Aminolevulinic Acid Biological Transport Cell Membrane Culture Media gamma-Aminobutyric Acid Kinetics Membrane Transport Proteins Saccharomyces cerevisiae Support, Non-U.S. Gov't Time Factors |
| description |
It has been previously reported that 5-aminolevulinic acid (ALA) and 4-aminobutyric acid (GABA) share a common permease in Saccharomyces cerevisiae (Bermúdez Moretti et al., 1993). The aim of the present work was to determine the relationship between the transport of these compounds in isolated cells. Assessment of amino acid incorporation was performed in S. cerevisiae using 14C-ALA or 3H-GABA. Initial rates of ALA incorporation in cells grown in the presence of 5 mM ALA and 5 mM GABA, were three to four times lower than in cells grown without supplements. Kinetic studies indicate that GABA competitively inhibits ALA transport. During the growth phase GABA uptake was also inhibited by 74% and 60% in the presence of ALA and GABA, respectively. These findings indicate that in S. cerevisiae the structurally related compounds, ALA and GABA, may be incorporated into the cells by a common carrier protein. Should this occur in other lukaryotic cells it may explain the neurotoxic effect attributed to ALA in the pathogenesis of acute porphyrias. © 1995. |
| format |
JOUR |
| author |
Bermúdez Moretti, M. Correa García, S.R. Chianelli, M.S. Ramos, E.H. Mattoon, J.R. Batlle, A. |
| author_facet |
Bermúdez Moretti, M. Correa García, S.R. Chianelli, M.S. Ramos, E.H. Mattoon, J.R. Batlle, A. |
| author_sort |
Bermúdez Moretti, M. |
| title |
Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae |
| title_short |
Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae |
| title_full |
Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae |
| title_fullStr |
Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae |
| title_full_unstemmed |
Evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into Saccharomyces cerevisiae |
| title_sort |
evidence that 4-aminobutyric acid and 5-aminolevulinic acid share a common transport system into saccharomyces cerevisiae |
| url |
http://hdl.handle.net/20.500.12110/paper_13572725_v27_n2_p169_BermudezMoretti |
| work_keys_str_mv |
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1807321051561984000 |