Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy

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The aim of the present study was to evaluate the photodynamic efficacy of a novel phthalocyanine derivate 2,3,9,10,16,17,23,24-octakis[(N,N-dimethylamino) ethylsulfanyl]phthalocyaninatozinc(II) (referred here as S1) using MCF-7c3 human breast cancer cells and the LM2 adenocarcinoma subcutaneously im...

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Autores principales:	Vittar, N.B.R., Prucca, C.G., Strassert, C., Awruch, J., Rivarola, V.A.
Formato:	JOUR
Lenguaje:	English
Materias:	Apoptosis Cancer Necrosis Photodynamic Therapy Photosensitizer dimyristoylphosphatidylcholine liposome phthalocyanine zinc adenocarcinoma animal cell animal model animal tissue antineoplastic activity apoptosis article breast cancer cancer cell culture cancer inhibition cell activation cell damage controlled study cytolysis drug delivery system drug distribution female human human cell incubation time internalization light damage light irradiance lysosome microscopy mitochondrion morphology mouse nonhuman photodynamic therapy photodynamics treatment response tumor volume Animals Cell Death Cell Line, Tumor Cell Survival Culture Media Darkness Female Humans Indoles Intracellular Space Mice Mice, Inbred BALB C Neoplasms Organometallic Compounds Photochemotherapy Solutions Spectrometry, Fluorescence Treatment Outcome Xenograft Model Antitumor Assays Animalia Mus
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13572725_v40_n10_p2192_Vittar
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_13572725_v40_n10_p2192_Vittar2023-10-03T16:10:31Z Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy Vittar, N.B.R. Prucca, C.G. Strassert, C. Awruch, J. Rivarola, V.A. Apoptosis Cancer Necrosis Photodynamic Therapy Photosensitizer dimyristoylphosphatidylcholine liposome phthalocyanine zinc adenocarcinoma animal cell animal model animal tissue antineoplastic activity apoptosis article breast cancer cancer cell culture cancer inhibition cell activation cell damage controlled study cytolysis drug delivery system drug distribution female human human cell incubation time internalization light damage light irradiance lysosome microscopy mitochondrion morphology mouse nonhuman photodynamic therapy photodynamics treatment response tumor volume Animals Cell Death Cell Line, Tumor Cell Survival Culture Media Darkness Female Humans Indoles Intracellular Space Mice Mice, Inbred BALB C Neoplasms Organometallic Compounds Photochemotherapy Solutions Spectrometry, Fluorescence Treatment Outcome Xenograft Model Antitumor Assays Animalia Mus The aim of the present study was to evaluate the photodynamic efficacy of a novel phthalocyanine derivate 2,3,9,10,16,17,23,24-octakis[(N,N-dimethylamino) ethylsulfanyl]phthalocyaninatozinc(II) (referred here as S1) using MCF-7c3 human breast cancer cells and the LM2 adenocarcinoma subcutaneously implanted in Balb/c mice as experimental models. The S1-l-α-dimyristoyl-phosphatidylcholine liposome was selected as the best delivery system because it showed greater internalization into cells (35 nmol/106 cells), relative to other liposomes. After 3 h incubation S1 was partially localized in lysosomes, the compartment that represented its primary photodamage site. The S1 treated cultures also revealed a degree of mitochondrial morphology alteration. Indeed, S1 leads to photokilling of the cells with different efficacies indicating that cell photoinactivation was dependent on both the phthalocyanine concentration and the light dose applied. Analyses of morphology and nuclear condensation level indicated that some of the cells exposed to photodynamic therapy were undergoing apoptosis within 8 h after treatment. To assess the in vivo effectiveness of S1, animals bearing tumors were treated with 0.2 mg/kg S1 followed 24 h later by 108 J cm-2 light at 600-800 nm and 60 mW cm-2,while other animals served as controls (no treatment, light alone, or S1 alone). All S1 treated tumors and none of the controls exhibited complete or partial responses, and these responses continued for the entire observation period of 12 days. Evaluation of tumor size showed that the treatment effectively delayed tumor growth. Light microscopy investigations of irradiated tumor specimens showed that S1 causes an early direct damage of malignant cells, largely via processes leading to random necrotic pathways. © 2008 Elsevier Ltd. All rights reserved. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13572725_v40_n10_p2192_Vittar
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	English
orig_language_str_mv	English
topic	Apoptosis Cancer Necrosis Photodynamic Therapy Photosensitizer dimyristoylphosphatidylcholine liposome phthalocyanine zinc adenocarcinoma animal cell animal model animal tissue antineoplastic activity apoptosis article breast cancer cancer cell culture cancer inhibition cell activation cell damage controlled study cytolysis drug delivery system drug distribution female human human cell incubation time internalization light damage light irradiance lysosome microscopy mitochondrion morphology mouse nonhuman photodynamic therapy photodynamics treatment response tumor volume Animals Cell Death Cell Line, Tumor Cell Survival Culture Media Darkness Female Humans Indoles Intracellular Space Mice Mice, Inbred BALB C Neoplasms Organometallic Compounds Photochemotherapy Solutions Spectrometry, Fluorescence Treatment Outcome Xenograft Model Antitumor Assays Animalia Mus
spellingShingle	Apoptosis Cancer Necrosis Photodynamic Therapy Photosensitizer dimyristoylphosphatidylcholine liposome phthalocyanine zinc adenocarcinoma animal cell animal model animal tissue antineoplastic activity apoptosis article breast cancer cancer cell culture cancer inhibition cell activation cell damage controlled study cytolysis drug delivery system drug distribution female human human cell incubation time internalization light damage light irradiance lysosome microscopy mitochondrion morphology mouse nonhuman photodynamic therapy photodynamics treatment response tumor volume Animals Cell Death Cell Line, Tumor Cell Survival Culture Media Darkness Female Humans Indoles Intracellular Space Mice Mice, Inbred BALB C Neoplasms Organometallic Compounds Photochemotherapy Solutions Spectrometry, Fluorescence Treatment Outcome Xenograft Model Antitumor Assays Animalia Mus Vittar, N.B.R. Prucca, C.G. Strassert, C. Awruch, J. Rivarola, V.A. Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy
topic_facet	Apoptosis Cancer Necrosis Photodynamic Therapy Photosensitizer dimyristoylphosphatidylcholine liposome phthalocyanine zinc adenocarcinoma animal cell animal model animal tissue antineoplastic activity apoptosis article breast cancer cancer cell culture cancer inhibition cell activation cell damage controlled study cytolysis drug delivery system drug distribution female human human cell incubation time internalization light damage light irradiance lysosome microscopy mitochondrion morphology mouse nonhuman photodynamic therapy photodynamics treatment response tumor volume Animals Cell Death Cell Line, Tumor Cell Survival Culture Media Darkness Female Humans Indoles Intracellular Space Mice Mice, Inbred BALB C Neoplasms Organometallic Compounds Photochemotherapy Solutions Spectrometry, Fluorescence Treatment Outcome Xenograft Model Antitumor Assays Animalia Mus
description	The aim of the present study was to evaluate the photodynamic efficacy of a novel phthalocyanine derivate 2,3,9,10,16,17,23,24-octakis[(N,N-dimethylamino) ethylsulfanyl]phthalocyaninatozinc(II) (referred here as S1) using MCF-7c3 human breast cancer cells and the LM2 adenocarcinoma subcutaneously implanted in Balb/c mice as experimental models. The S1-l-α-dimyristoyl-phosphatidylcholine liposome was selected as the best delivery system because it showed greater internalization into cells (35 nmol/106 cells), relative to other liposomes. After 3 h incubation S1 was partially localized in lysosomes, the compartment that represented its primary photodamage site. The S1 treated cultures also revealed a degree of mitochondrial morphology alteration. Indeed, S1 leads to photokilling of the cells with different efficacies indicating that cell photoinactivation was dependent on both the phthalocyanine concentration and the light dose applied. Analyses of morphology and nuclear condensation level indicated that some of the cells exposed to photodynamic therapy were undergoing apoptosis within 8 h after treatment. To assess the in vivo effectiveness of S1, animals bearing tumors were treated with 0.2 mg/kg S1 followed 24 h later by 108 J cm-2 light at 600-800 nm and 60 mW cm-2,while other animals served as controls (no treatment, light alone, or S1 alone). All S1 treated tumors and none of the controls exhibited complete or partial responses, and these responses continued for the entire observation period of 12 days. Evaluation of tumor size showed that the treatment effectively delayed tumor growth. Light microscopy investigations of irradiated tumor specimens showed that S1 causes an early direct damage of malignant cells, largely via processes leading to random necrotic pathways. © 2008 Elsevier Ltd. All rights reserved.
format	JOUR
author	Vittar, N.B.R. Prucca, C.G. Strassert, C. Awruch, J. Rivarola, V.A.
author_facet	Vittar, N.B.R. Prucca, C.G. Strassert, C. Awruch, J. Rivarola, V.A.
author_sort	Vittar, N.B.R.
title	Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy
title_short	Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy
title_full	Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy
title_fullStr	Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy
title_full_unstemmed	Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy
title_sort	cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy
url	http://hdl.handle.net/20.500.12110/paper_13572725_v40_n10_p2192_Vittar
work_keys_str_mv	AT vittarnbr cellularinactivationandantitumorefficacyofanewzincphthalocyaninewithpotentialuseinphotodynamictherapy AT pruccacg cellularinactivationandantitumorefficacyofanewzincphthalocyaninewithpotentialuseinphotodynamictherapy AT strassertc cellularinactivationandantitumorefficacyofanewzincphthalocyaninewithpotentialuseinphotodynamictherapy AT awruchj cellularinactivationandantitumorefficacyofanewzincphthalocyaninewithpotentialuseinphotodynamictherapy AT rivarolava cellularinactivationandantitumorefficacyofanewzincphthalocyaninewithpotentialuseinphotodynamictherapy
_version_	1807320208569794560

Cellular inactivation and antitumor efficacy of a new zinc phthalocyanine with potential use in photodynamic therapy

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