Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production
Aims: To study phosphate solubilization in Penicillium purpurogenum as function of medium pH, and carbon and nitrogen concentrations. Methods and Results: Tricalcium phosphate (CP) solubilization efficiency of P. purpurogenum was evaluated at acid or alkaline pH using different C and N sources. Gluc...
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todo:paper_13645072_v110_n5_p1215_Scervino2023-10-03T16:11:01Z Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production Scervino, J.M. Papinutti, V.L. Godoy, M.S. Rodriguez, M.A. Della Monica, I. Recchi, M. Pettinari, M.J. Godeas, A.M. Organic acids Penicillium purpurogenum Phosphate-solubilizing micro-organisms Phosphorus ammonium sulfate calcium carbon carboxylic acid fructose gluconic acid glucose nitrogen phosphate alkalinity calcium carbon concentration (composition) experimental design fertilizer application fungus glucose inoculation nitrogen optimization organic acid pH phosphate soil ecosystem soil microorganism solubilization alkalinity article bioprocess ecosystem fungus culture fungus growth inoculation nonhuman Penicillium purpurogenum pH solubilization Calcium Phosphates Carbon Culture Media Gluconates Hydrogen-Ion Concentration Nitrogen Penicillium Soil Microbiology Solubility Penicillium purpurogenum Aims: To study phosphate solubilization in Penicillium purpurogenum as function of medium pH, and carbon and nitrogen concentrations. Methods and Results: Tricalcium phosphate (CP) solubilization efficiency of P. purpurogenum was evaluated at acid or alkaline pH using different C and N sources. Glucose- and (NH4)2SO4-based media showed the highest P solubilization values followed by fructose. P. purpurogenum solubilizing ability was higher in cultures grown at pH 6·5 than cultures at pH 8·5. Organic acids were detected in both alkaline and neutral media, but the relative percentages of each organic acid differed. Highest P release coincided with the highest organic acids production peak, especially gluconic acid. When P. purpurogenum grew in alkaline media, the nature and concentration of organic acids changed at different N and C concentrations. A factorial categorical experimental design showed that the highest P-solubilizing activity, coinciding with the highest organic acid production, corresponded to the highest C concentration and lowest N concentration. Conclusions: The results described in the present study show that medium pH and carbon and nitrogen concentrations modulate the P solubilization efficiency of P. purpurogenum through the production of organic acids and particularly that of gluconic acid. In the P solubilization optimization studies, glucose and (NH4)2SO4 as C and N sources allowed a higher solubilization efficiency at high pH. Significance and Impact of the Study: This organism is a potentially proficient soil inoculant, especially in P-poor alkaline soils where other P solubilizers fail to release soluble P. Further work is necessary to elucidate whether these results can be extrapolated to natural soil ecosystems, where different pH values are present. Penicillium purpurogenum could be used to develop a bioprocess for the manufacture of phosphatic fertilizer with phosphate calcium minerals. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology. Fil:Scervino, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Papinutti, V.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguez, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Della Monica, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13645072_v110_n5_p1215_Scervino |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Organic acids Penicillium purpurogenum Phosphate-solubilizing micro-organisms Phosphorus ammonium sulfate calcium carbon carboxylic acid fructose gluconic acid glucose nitrogen phosphate alkalinity calcium carbon concentration (composition) experimental design fertilizer application fungus glucose inoculation nitrogen optimization organic acid pH phosphate soil ecosystem soil microorganism solubilization alkalinity article bioprocess ecosystem fungus culture fungus growth inoculation nonhuman Penicillium purpurogenum pH solubilization Calcium Phosphates Carbon Culture Media Gluconates Hydrogen-Ion Concentration Nitrogen Penicillium Soil Microbiology Solubility Penicillium purpurogenum |
| spellingShingle |
Organic acids Penicillium purpurogenum Phosphate-solubilizing micro-organisms Phosphorus ammonium sulfate calcium carbon carboxylic acid fructose gluconic acid glucose nitrogen phosphate alkalinity calcium carbon concentration (composition) experimental design fertilizer application fungus glucose inoculation nitrogen optimization organic acid pH phosphate soil ecosystem soil microorganism solubilization alkalinity article bioprocess ecosystem fungus culture fungus growth inoculation nonhuman Penicillium purpurogenum pH solubilization Calcium Phosphates Carbon Culture Media Gluconates Hydrogen-Ion Concentration Nitrogen Penicillium Soil Microbiology Solubility Penicillium purpurogenum Scervino, J.M. Papinutti, V.L. Godoy, M.S. Rodriguez, M.A. Della Monica, I. Recchi, M. Pettinari, M.J. Godeas, A.M. Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production |
| topic_facet |
Organic acids Penicillium purpurogenum Phosphate-solubilizing micro-organisms Phosphorus ammonium sulfate calcium carbon carboxylic acid fructose gluconic acid glucose nitrogen phosphate alkalinity calcium carbon concentration (composition) experimental design fertilizer application fungus glucose inoculation nitrogen optimization organic acid pH phosphate soil ecosystem soil microorganism solubilization alkalinity article bioprocess ecosystem fungus culture fungus growth inoculation nonhuman Penicillium purpurogenum pH solubilization Calcium Phosphates Carbon Culture Media Gluconates Hydrogen-Ion Concentration Nitrogen Penicillium Soil Microbiology Solubility Penicillium purpurogenum |
| description |
Aims: To study phosphate solubilization in Penicillium purpurogenum as function of medium pH, and carbon and nitrogen concentrations. Methods and Results: Tricalcium phosphate (CP) solubilization efficiency of P. purpurogenum was evaluated at acid or alkaline pH using different C and N sources. Glucose- and (NH4)2SO4-based media showed the highest P solubilization values followed by fructose. P. purpurogenum solubilizing ability was higher in cultures grown at pH 6·5 than cultures at pH 8·5. Organic acids were detected in both alkaline and neutral media, but the relative percentages of each organic acid differed. Highest P release coincided with the highest organic acids production peak, especially gluconic acid. When P. purpurogenum grew in alkaline media, the nature and concentration of organic acids changed at different N and C concentrations. A factorial categorical experimental design showed that the highest P-solubilizing activity, coinciding with the highest organic acid production, corresponded to the highest C concentration and lowest N concentration. Conclusions: The results described in the present study show that medium pH and carbon and nitrogen concentrations modulate the P solubilization efficiency of P. purpurogenum through the production of organic acids and particularly that of gluconic acid. In the P solubilization optimization studies, glucose and (NH4)2SO4 as C and N sources allowed a higher solubilization efficiency at high pH. Significance and Impact of the Study: This organism is a potentially proficient soil inoculant, especially in P-poor alkaline soils where other P solubilizers fail to release soluble P. Further work is necessary to elucidate whether these results can be extrapolated to natural soil ecosystems, where different pH values are present. Penicillium purpurogenum could be used to develop a bioprocess for the manufacture of phosphatic fertilizer with phosphate calcium minerals. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology. |
| format |
JOUR |
| author |
Scervino, J.M. Papinutti, V.L. Godoy, M.S. Rodriguez, M.A. Della Monica, I. Recchi, M. Pettinari, M.J. Godeas, A.M. |
| author_facet |
Scervino, J.M. Papinutti, V.L. Godoy, M.S. Rodriguez, M.A. Della Monica, I. Recchi, M. Pettinari, M.J. Godeas, A.M. |
| author_sort |
Scervino, J.M. |
| title |
Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production |
| title_short |
Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production |
| title_full |
Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production |
| title_fullStr |
Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production |
| title_full_unstemmed |
Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production |
| title_sort |
medium ph, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of penicillium purpurogenum through organic acid production |
| url |
http://hdl.handle.net/20.500.12110/paper_13645072_v110_n5_p1215_Scervino |
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