Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value-added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails. Methods and...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_13645072_v126_n3_p811_Piccinni |
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todo:paper_13645072_v126_n3_p811_Piccinni2023-10-03T16:11:04Z Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates Piccinni, F.E. Ontañon, O.M. Ghio, S. Sauka, D.H. Talia, P.M. Rivarola, M.L. Valacco, M.P. Campos, E. cazymes cellulomonas gh10 glucanases glycosyl hydrolases lignocellulose secretome xylanases arabinofuranosidase bacterial DNA bacterial enzyme bacterial RNA carboxymethylcellulose exoglucanase glucan synthase glycosidase lignocellulose RNA 16S unclassified drug xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase xyloglucanase bacterium biofuel biotechnology biotransformation cellulose crop residue enzyme activity genome lignin secretion straw Article bacterial genome bacterial growth bacterium culture biomass Cellulomonas Cellulomonas aerilata Cellulomonas biazotea Cellulomonas bogoriensis Cellulomonas carbonis Cellulomonas cellasea Cellulomonas chitinilytica Cellulomonas composti Cellulomonas denverensis Cellulomonas fimi Cellulomonas flavigena Cellulomonas gelida Cellulomonas hominis Cellulomonas humilata Cellulomonas iranensis Cellulomonas marina Cellulomonas massiliensis Cellulomonas oligotrophica Cellulomonas pakistanensis Cellulomonas phragmiteti Cellulomonas soli Cellulomonas terrae Cellulomonas uda Cellulomonas xylanilytica controlled study DNA DNA hybridization enzyme activity enzyme substrate Micrococcus luteus nonhuman phylogeny protein secretion RNA sequence sugarcane Cellulomonas Cellulomonas sp. Saccharum Triticum aestivum Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value-added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo- and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β-xylosidase, a β-glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose-degrading enzymes applicable to bioprocessing and biotechnological industries. © 2018 The Society for Applied Microbiology JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13645072_v126_n3_p811_Piccinni |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cazymes cellulomonas gh10 glucanases glycosyl hydrolases lignocellulose secretome xylanases arabinofuranosidase bacterial DNA bacterial enzyme bacterial RNA carboxymethylcellulose exoglucanase glucan synthase glycosidase lignocellulose RNA 16S unclassified drug xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase xyloglucanase bacterium biofuel biotechnology biotransformation cellulose crop residue enzyme activity genome lignin secretion straw Article bacterial genome bacterial growth bacterium culture biomass Cellulomonas Cellulomonas aerilata Cellulomonas biazotea Cellulomonas bogoriensis Cellulomonas carbonis Cellulomonas cellasea Cellulomonas chitinilytica Cellulomonas composti Cellulomonas denverensis Cellulomonas fimi Cellulomonas flavigena Cellulomonas gelida Cellulomonas hominis Cellulomonas humilata Cellulomonas iranensis Cellulomonas marina Cellulomonas massiliensis Cellulomonas oligotrophica Cellulomonas pakistanensis Cellulomonas phragmiteti Cellulomonas soli Cellulomonas terrae Cellulomonas uda Cellulomonas xylanilytica controlled study DNA DNA hybridization enzyme activity enzyme substrate Micrococcus luteus nonhuman phylogeny protein secretion RNA sequence sugarcane Cellulomonas Cellulomonas sp. Saccharum Triticum aestivum |
| spellingShingle |
cazymes cellulomonas gh10 glucanases glycosyl hydrolases lignocellulose secretome xylanases arabinofuranosidase bacterial DNA bacterial enzyme bacterial RNA carboxymethylcellulose exoglucanase glucan synthase glycosidase lignocellulose RNA 16S unclassified drug xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase xyloglucanase bacterium biofuel biotechnology biotransformation cellulose crop residue enzyme activity genome lignin secretion straw Article bacterial genome bacterial growth bacterium culture biomass Cellulomonas Cellulomonas aerilata Cellulomonas biazotea Cellulomonas bogoriensis Cellulomonas carbonis Cellulomonas cellasea Cellulomonas chitinilytica Cellulomonas composti Cellulomonas denverensis Cellulomonas fimi Cellulomonas flavigena Cellulomonas gelida Cellulomonas hominis Cellulomonas humilata Cellulomonas iranensis Cellulomonas marina Cellulomonas massiliensis Cellulomonas oligotrophica Cellulomonas pakistanensis Cellulomonas phragmiteti Cellulomonas soli Cellulomonas terrae Cellulomonas uda Cellulomonas xylanilytica controlled study DNA DNA hybridization enzyme activity enzyme substrate Micrococcus luteus nonhuman phylogeny protein secretion RNA sequence sugarcane Cellulomonas Cellulomonas sp. Saccharum Triticum aestivum Piccinni, F.E. Ontañon, O.M. Ghio, S. Sauka, D.H. Talia, P.M. Rivarola, M.L. Valacco, M.P. Campos, E. Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| topic_facet |
cazymes cellulomonas gh10 glucanases glycosyl hydrolases lignocellulose secretome xylanases arabinofuranosidase bacterial DNA bacterial enzyme bacterial RNA carboxymethylcellulose exoglucanase glucan synthase glycosidase lignocellulose RNA 16S unclassified drug xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase xyloglucanase bacterium biofuel biotechnology biotransformation cellulose crop residue enzyme activity genome lignin secretion straw Article bacterial genome bacterial growth bacterium culture biomass Cellulomonas Cellulomonas aerilata Cellulomonas biazotea Cellulomonas bogoriensis Cellulomonas carbonis Cellulomonas cellasea Cellulomonas chitinilytica Cellulomonas composti Cellulomonas denverensis Cellulomonas fimi Cellulomonas flavigena Cellulomonas gelida Cellulomonas hominis Cellulomonas humilata Cellulomonas iranensis Cellulomonas marina Cellulomonas massiliensis Cellulomonas oligotrophica Cellulomonas pakistanensis Cellulomonas phragmiteti Cellulomonas soli Cellulomonas terrae Cellulomonas uda Cellulomonas xylanilytica controlled study DNA DNA hybridization enzyme activity enzyme substrate Micrococcus luteus nonhuman phylogeny protein secretion RNA sequence sugarcane Cellulomonas Cellulomonas sp. Saccharum Triticum aestivum |
| description |
Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value-added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo- and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β-xylosidase, a β-glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose-degrading enzymes applicable to bioprocessing and biotechnological industries. © 2018 The Society for Applied Microbiology |
| format |
JOUR |
| author |
Piccinni, F.E. Ontañon, O.M. Ghio, S. Sauka, D.H. Talia, P.M. Rivarola, M.L. Valacco, M.P. Campos, E. |
| author_facet |
Piccinni, F.E. Ontañon, O.M. Ghio, S. Sauka, D.H. Talia, P.M. Rivarola, M.L. Valacco, M.P. Campos, E. |
| author_sort |
Piccinni, F.E. |
| title |
Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title_short |
Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title_full |
Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title_fullStr |
Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title_full_unstemmed |
Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates |
| title_sort |
secretome profile of cellulomonas sp. b6 growing on lignocellulosic substrates |
| url |
http://hdl.handle.net/20.500.12110/paper_13645072_v126_n3_p811_Piccinni |
| work_keys_str_mv |
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| _version_ |
1807323543332978688 |