Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin

Microtubules (MT) are formed by the assembly of α- and β-tubulins and MT-associated proteins. We characterized the effects of pharmaceutical formulations containing the microtubule disruptors thiabendazole (TBZ) and griseofulvin (GF) on the mitotic machinery of plant (A. cepa) meristematic cells. GF...

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Autores principales:	Andrioli, N.B., Soloneski, S., Larramendy, M.L., Mudry, M.D.
Formato:	JOUR
Materias:	Allium cepa Griseofulvin Microtubule arrays Thiabendazole Tubulin beta tubulin griseofulvin tiabendazole anthelmintic agent antifungal agent anaphase article cell damage cell division chromosome aberration chromosome analysis controlled study environmental exposure immunofluorescence micronucleus microtubule mini cell mitosis index mitosis spindle nonhuman nuclear buds onion polyploidy priority journal root cell telophase cytology DNA damage drug effects genetics meristem metabolism metaphase plant cell plant chromosome Anthelmintics Antifungal Agents Chromosomes, Plant DNA Damage Meristem Metaphase Microtubules Onions Plant Cells Polyploidy
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13835718_v772_n_p1_Andrioli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_13835718_v772_n_p1_Andrioli
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spelling	todo:paper_13835718_v772_n_p1_Andrioli2023-10-03T16:11:57Z Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin Andrioli, N.B. Soloneski, S. Larramendy, M.L. Mudry, M.D. Allium cepa Griseofulvin Microtubule arrays Thiabendazole Tubulin beta tubulin griseofulvin tiabendazole anthelmintic agent antifungal agent griseofulvin tiabendazole anaphase article cell damage cell division chromosome aberration chromosome analysis controlled study environmental exposure immunofluorescence micronucleus microtubule mini cell mitosis index mitosis spindle nonhuman nuclear buds onion polyploidy priority journal root cell telophase cytology DNA damage drug effects genetics meristem metabolism metaphase microtubule onion plant cell plant chromosome Anthelmintics Antifungal Agents Chromosomes, Plant DNA Damage Griseofulvin Meristem Metaphase Microtubules Onions Plant Cells Polyploidy Thiabendazole Microtubules (MT) are formed by the assembly of α- and β-tubulins and MT-associated proteins. We characterized the effects of pharmaceutical formulations containing the microtubule disruptors thiabendazole (TBZ) and griseofulvin (GF) on the mitotic machinery of plant (A. cepa) meristematic cells. GF concentrations between 10 and 250. μg/ml were tested. GF induced mitotic index inhibition and genotoxic effects, including chromosome fragments, bridges, lagged chromosomes, C-metaphases, tripolar cell division, disorganized anaphases and nuclear abnormalities in interphase cells. Efects on the mitotic machinery were studied by direct immunofluorescence with β-tubulin labeling and by DNA counterstaining with 4',6-diamidino-2-phenylindole (DAPI). Exposure of meristematic root cells to TBZ or GF, 100. μg/ml, caused microtubular damage which led to abnormal MT arrays. Our results suggest that GF induces abnormalities in spindle symmetry/polarity, while TBZ causes chromosome missegregation, polyploidy, and lack of cytokinesis. © 2014 Elsevier B.V. Fil:Andrioli, N.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13835718_v772_n_p1_Andrioli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Allium cepa Griseofulvin Microtubule arrays Thiabendazole Tubulin beta tubulin griseofulvin tiabendazole anthelmintic agent antifungal agent griseofulvin tiabendazole anaphase article cell damage cell division chromosome aberration chromosome analysis controlled study environmental exposure immunofluorescence micronucleus microtubule mini cell mitosis index mitosis spindle nonhuman nuclear buds onion polyploidy priority journal root cell telophase cytology DNA damage drug effects genetics meristem metabolism metaphase microtubule onion plant cell plant chromosome Anthelmintics Antifungal Agents Chromosomes, Plant DNA Damage Griseofulvin Meristem Metaphase Microtubules Onions Plant Cells Polyploidy Thiabendazole
spellingShingle	Allium cepa Griseofulvin Microtubule arrays Thiabendazole Tubulin beta tubulin griseofulvin tiabendazole anthelmintic agent antifungal agent griseofulvin tiabendazole anaphase article cell damage cell division chromosome aberration chromosome analysis controlled study environmental exposure immunofluorescence micronucleus microtubule mini cell mitosis index mitosis spindle nonhuman nuclear buds onion polyploidy priority journal root cell telophase cytology DNA damage drug effects genetics meristem metabolism metaphase microtubule onion plant cell plant chromosome Anthelmintics Antifungal Agents Chromosomes, Plant DNA Damage Griseofulvin Meristem Metaphase Microtubules Onions Plant Cells Polyploidy Thiabendazole Andrioli, N.B. Soloneski, S. Larramendy, M.L. Mudry, M.D. Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin
topic_facet	Allium cepa Griseofulvin Microtubule arrays Thiabendazole Tubulin beta tubulin griseofulvin tiabendazole anthelmintic agent antifungal agent griseofulvin tiabendazole anaphase article cell damage cell division chromosome aberration chromosome analysis controlled study environmental exposure immunofluorescence micronucleus microtubule mini cell mitosis index mitosis spindle nonhuman nuclear buds onion polyploidy priority journal root cell telophase cytology DNA damage drug effects genetics meristem metabolism metaphase microtubule onion plant cell plant chromosome Anthelmintics Antifungal Agents Chromosomes, Plant DNA Damage Griseofulvin Meristem Metaphase Microtubules Onions Plant Cells Polyploidy Thiabendazole
description	Microtubules (MT) are formed by the assembly of α- and β-tubulins and MT-associated proteins. We characterized the effects of pharmaceutical formulations containing the microtubule disruptors thiabendazole (TBZ) and griseofulvin (GF) on the mitotic machinery of plant (A. cepa) meristematic cells. GF concentrations between 10 and 250. μg/ml were tested. GF induced mitotic index inhibition and genotoxic effects, including chromosome fragments, bridges, lagged chromosomes, C-metaphases, tripolar cell division, disorganized anaphases and nuclear abnormalities in interphase cells. Efects on the mitotic machinery were studied by direct immunofluorescence with β-tubulin labeling and by DNA counterstaining with 4',6-diamidino-2-phenylindole (DAPI). Exposure of meristematic root cells to TBZ or GF, 100. μg/ml, caused microtubular damage which led to abnormal MT arrays. Our results suggest that GF induces abnormalities in spindle symmetry/polarity, while TBZ causes chromosome missegregation, polyploidy, and lack of cytokinesis. © 2014 Elsevier B.V.
format	JOUR
author	Andrioli, N.B. Soloneski, S. Larramendy, M.L. Mudry, M.D.
author_facet	Andrioli, N.B. Soloneski, S. Larramendy, M.L. Mudry, M.D.
author_sort	Andrioli, N.B.
title	Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin
title_short	Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin
title_full	Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin
title_fullStr	Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin
title_full_unstemmed	Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin
title_sort	induction of microtubule damage in allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin
url	http://hdl.handle.net/20.500.12110/paper_13835718_v772_n_p1_Andrioli
work_keys_str_mv	AT andriolinb inductionofmicrotubuledamageinalliumcepameristematiccellsbypharmaceuticalformulationsofthiabendazoleandgriseofulvin AT soloneskis inductionofmicrotubuledamageinalliumcepameristematiccellsbypharmaceuticalformulationsofthiabendazoleandgriseofulvin AT larramendyml inductionofmicrotubuledamageinalliumcepameristematiccellsbypharmaceuticalformulationsofthiabendazoleandgriseofulvin AT mudrymd inductionofmicrotubuledamageinalliumcepameristematiccellsbypharmaceuticalformulationsofthiabendazoleandgriseofulvin
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Induction of microtubule damage in Allium cepa meristematic cells by pharmaceutical formulations of thiabendazole and griseofulvin

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