The first chemical synthesis of UDP[6-3H]-α-D- galactofuranose

Galactofuranose metabolism is a good target for the development of novel chemotherapeutic agents for the treatment of some microbial infections. This is a valid objective because galactofuranose is absent in mammals. Two enzymes are involved in the biosynthesis of molecules containing galactofuranos...

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Detalles Bibliográficos
Autores principales:	Mariño, K., Marino, C., Lima, C., Baldoni, L., De Lederkremer, R.M.
Formato:	JOUR
Materias:	Carbohydrates Galactofuranosyltransferase Isotopic labeling Nucleotides UDP-Galf
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1434193X_v_n14_p2958_Marino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_1434193X_v_n14_p2958_Marino2023-10-03T16:14:15Z The first chemical synthesis of UDP[6-3H]-α-D- galactofuranose Mariño, K. Marino, C. Lima, C. Baldoni, L. De Lederkremer, R.M. Carbohydrates Galactofuranosyltransferase Isotopic labeling Nucleotides UDP-Galf Galactofuranose metabolism is a good target for the development of novel chemotherapeutic agents for the treatment of some microbial infections. This is a valid objective because galactofuranose is absent in mammals. Two enzymes are involved in the biosynthesis of molecules containing galactofuranose: a mutase, which catalyzes the interconversion of UDP-Galp and UDP-Galf, and D-galactofuranosyltransferases. The mechanism of action of the mutase and its inhibition is currently being investigated, whereas studies on the galactofuranosyltransferases have been hampered by the lack of a labeled galactofuranose nucleotide. In the present work we describe the chemical synthesis of UDP-α-D-[6-3H]Galf and we prove its effectiveness for incorporation of radioactive galactofuranose into a natural acceptor. This is the first report on the chemical synthesis of a labeled donor of galactofuranose with the potential for studying the galactofuranosyltransferases independently from the UDP-Galp mutase. © Wiley-VCH Verlag GmbH & Co. KGaA, 2005. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1434193X_v_n14_p2958_Marino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Carbohydrates Galactofuranosyltransferase Isotopic labeling Nucleotides UDP-Galf
spellingShingle	Carbohydrates Galactofuranosyltransferase Isotopic labeling Nucleotides UDP-Galf Mariño, K. Marino, C. Lima, C. Baldoni, L. De Lederkremer, R.M. The first chemical synthesis of UDP[6-3H]-α-D- galactofuranose
topic_facet	Carbohydrates Galactofuranosyltransferase Isotopic labeling Nucleotides UDP-Galf
description	Galactofuranose metabolism is a good target for the development of novel chemotherapeutic agents for the treatment of some microbial infections. This is a valid objective because galactofuranose is absent in mammals. Two enzymes are involved in the biosynthesis of molecules containing galactofuranose: a mutase, which catalyzes the interconversion of UDP-Galp and UDP-Galf, and D-galactofuranosyltransferases. The mechanism of action of the mutase and its inhibition is currently being investigated, whereas studies on the galactofuranosyltransferases have been hampered by the lack of a labeled galactofuranose nucleotide. In the present work we describe the chemical synthesis of UDP-α-D-[6-3H]Galf and we prove its effectiveness for incorporation of radioactive galactofuranose into a natural acceptor. This is the first report on the chemical synthesis of a labeled donor of galactofuranose with the potential for studying the galactofuranosyltransferases independently from the UDP-Galp mutase. © Wiley-VCH Verlag GmbH & Co. KGaA, 2005.
format	JOUR
author	Mariño, K. Marino, C. Lima, C. Baldoni, L. De Lederkremer, R.M.
author_facet	Mariño, K. Marino, C. Lima, C. Baldoni, L. De Lederkremer, R.M.
author_sort	Mariño, K.
title	The first chemical synthesis of UDP[6-3H]-α-D- galactofuranose
title_short	The first chemical synthesis of UDP[6-3H]-α-D- galactofuranose
title_full	The first chemical synthesis of UDP[6-3H]-α-D- galactofuranose
title_fullStr	The first chemical synthesis of UDP[6-3H]-α-D- galactofuranose
title_full_unstemmed	The first chemical synthesis of UDP[6-3H]-α-D- galactofuranose
title_sort	first chemical synthesis of udp[6-3h]-α-d- galactofuranose
url	http://hdl.handle.net/20.500.12110/paper_1434193X_v_n14_p2958_Marino
work_keys_str_mv	AT marinok thefirstchemicalsynthesisofudp63hadgalactofuranose AT marinoc thefirstchemicalsynthesisofudp63hadgalactofuranose AT limac thefirstchemicalsynthesisofudp63hadgalactofuranose AT baldonil thefirstchemicalsynthesisofudp63hadgalactofuranose AT delederkremerrm thefirstchemicalsynthesisofudp63hadgalactofuranose AT marinok firstchemicalsynthesisofudp63hadgalactofuranose AT marinoc firstchemicalsynthesisofudp63hadgalactofuranose AT limac firstchemicalsynthesisofudp63hadgalactofuranose AT baldonil firstchemicalsynthesisofudp63hadgalactofuranose AT delederkremerrm firstchemicalsynthesisofudp63hadgalactofuranose
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The first chemical synthesis of UDP[6-3H]-α-D- galactofuranose

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