Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness

Potato (Solanum tuberosum L.) tubers are an excellent staple food due to its high nutritional value. When the tuber reaches physiological competence, sprouting proceeds accompanied by changes at mRNA and protein levels. Potato tubers become a source of carbon and energy until sprouts are capable of...

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Autores principales:	Grandellis, C., Giammaria, V., Fantino, E., Cerrudo, I., Bachmann, S., Santin, F., Ulloa, R.M.
Formato:	JOUR
Materias:	Light/dark conditions Protease inhibitors Solanum tuberosum TIGR 10K microarrays Tuber sprouting chloroplast protein cysteine proteinase cysteine proteinase inhibitor glyceraldehyde 3 phosphate dehydrogenase metallocarboxypeptidase inhibitor peptide hydrolase inhibitor proton transporting adenosine triphosphate synthase ribulosebisphosphate carboxylase transcriptome unclassified drug plant protein Article cell metabolism cell wall metabolism correlational study darkness enzyme activity feedback system GAPDH gene gene expression genetic association genetic procedures light microarray analysis nonhuman Northern blotting photosystem II plant gene plant genome plant growth potato priority journal protein expression protein metabolism protein synthesis inhibition RCA gene reverse transcription polymerase chain reaction senescence sprout sprouting transcript profiling upregulation biosynthesis gene expression regulation genetics growth, development and aging photosynthesis plant tuber seedling transcription initiation Cysteine Proteinase Inhibitors Darkness Gene Expression Regulation, Plant Light Photosynthesis Plant Proteins Plant Tubers Seedlings Transcriptional Activation
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1438793X_v16_n4_p399_Grandellis
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_1438793X_v16_n4_p399_Grandellis2023-10-03T16:16:12Z Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness Grandellis, C. Giammaria, V. Fantino, E. Cerrudo, I. Bachmann, S. Santin, F. Ulloa, R.M. Light/dark conditions Protease inhibitors Solanum tuberosum TIGR 10K microarrays Tuber sprouting chloroplast protein cysteine proteinase cysteine proteinase inhibitor glyceraldehyde 3 phosphate dehydrogenase metallocarboxypeptidase inhibitor peptide hydrolase inhibitor proton transporting adenosine triphosphate synthase ribulosebisphosphate carboxylase transcriptome unclassified drug cysteine proteinase inhibitor plant protein Article cell metabolism cell wall metabolism correlational study darkness enzyme activity feedback system GAPDH gene gene expression genetic association genetic procedures light microarray analysis nonhuman Northern blotting photosystem II plant gene plant genome plant growth potato priority journal protein expression protein metabolism protein synthesis inhibition RCA gene reverse transcription polymerase chain reaction senescence sprout sprouting transcript profiling upregulation biosynthesis darkness gene expression regulation genetics growth, development and aging photosynthesis plant tuber seedling transcription initiation Cysteine Proteinase Inhibitors Darkness Gene Expression Regulation, Plant Light Photosynthesis Plant Proteins Plant Tubers Seedlings Solanum tuberosum Transcriptional Activation Potato (Solanum tuberosum L.) tubers are an excellent staple food due to its high nutritional value. When the tuber reaches physiological competence, sprouting proceeds accompanied by changes at mRNA and protein levels. Potato tubers become a source of carbon and energy until sprouts are capable of independent growth. Transcript profiling of sprouts grown under continuous light or dark conditions was performed using the TIGR 10K EST Solanaceae microarray. The profiles analyzed show a core of highly expressed transcripts that are associated to the reactivation of growth. Under light conditions, the photosynthetic machinery was fully activated; the highest up-regulation was observed for the Rubisco activase (RCA), the glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the Photosystem II 22 kDa protein (CP22) genes, among others. On the other hand, sprouts exposed to continuous darkness elongate longer, and after extended darkness, synthesis of chloroplast components was repressed, the expression of proteases was reduced while genes encoding cysteine protease inhibitors (CPIs) and metallocarboxypeptidase inhibitors (MPIs) were strongly induced. Northern blot and RT-PCR analysis confirmed that MPI levels correlated with the length of the dark period; however, CPI expression was strong only after longer periods of darkness, suggesting a feedback loop (regulation mechanism) in response to dark-induced senescence. Prevention of cysteine protease activity in etiolated sprouts exposed to extended darkness could delay senescence until they emerge to light. © 2016, Springer-Verlag Berlin Heidelberg. Fil:Grandellis, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giammaria, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bachmann, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1438793X_v16_n4_p399_Grandellis
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Light/dark conditions Protease inhibitors Solanum tuberosum TIGR 10K microarrays Tuber sprouting chloroplast protein cysteine proteinase cysteine proteinase inhibitor glyceraldehyde 3 phosphate dehydrogenase metallocarboxypeptidase inhibitor peptide hydrolase inhibitor proton transporting adenosine triphosphate synthase ribulosebisphosphate carboxylase transcriptome unclassified drug cysteine proteinase inhibitor plant protein Article cell metabolism cell wall metabolism correlational study darkness enzyme activity feedback system GAPDH gene gene expression genetic association genetic procedures light microarray analysis nonhuman Northern blotting photosystem II plant gene plant genome plant growth potato priority journal protein expression protein metabolism protein synthesis inhibition RCA gene reverse transcription polymerase chain reaction senescence sprout sprouting transcript profiling upregulation biosynthesis darkness gene expression regulation genetics growth, development and aging photosynthesis plant tuber seedling transcription initiation Cysteine Proteinase Inhibitors Darkness Gene Expression Regulation, Plant Light Photosynthesis Plant Proteins Plant Tubers Seedlings Solanum tuberosum Transcriptional Activation
spellingShingle	Light/dark conditions Protease inhibitors Solanum tuberosum TIGR 10K microarrays Tuber sprouting chloroplast protein cysteine proteinase cysteine proteinase inhibitor glyceraldehyde 3 phosphate dehydrogenase metallocarboxypeptidase inhibitor peptide hydrolase inhibitor proton transporting adenosine triphosphate synthase ribulosebisphosphate carboxylase transcriptome unclassified drug cysteine proteinase inhibitor plant protein Article cell metabolism cell wall metabolism correlational study darkness enzyme activity feedback system GAPDH gene gene expression genetic association genetic procedures light microarray analysis nonhuman Northern blotting photosystem II plant gene plant genome plant growth potato priority journal protein expression protein metabolism protein synthesis inhibition RCA gene reverse transcription polymerase chain reaction senescence sprout sprouting transcript profiling upregulation biosynthesis darkness gene expression regulation genetics growth, development and aging photosynthesis plant tuber seedling transcription initiation Cysteine Proteinase Inhibitors Darkness Gene Expression Regulation, Plant Light Photosynthesis Plant Proteins Plant Tubers Seedlings Solanum tuberosum Transcriptional Activation Grandellis, C. Giammaria, V. Fantino, E. Cerrudo, I. Bachmann, S. Santin, F. Ulloa, R.M. Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness
topic_facet	Light/dark conditions Protease inhibitors Solanum tuberosum TIGR 10K microarrays Tuber sprouting chloroplast protein cysteine proteinase cysteine proteinase inhibitor glyceraldehyde 3 phosphate dehydrogenase metallocarboxypeptidase inhibitor peptide hydrolase inhibitor proton transporting adenosine triphosphate synthase ribulosebisphosphate carboxylase transcriptome unclassified drug cysteine proteinase inhibitor plant protein Article cell metabolism cell wall metabolism correlational study darkness enzyme activity feedback system GAPDH gene gene expression genetic association genetic procedures light microarray analysis nonhuman Northern blotting photosystem II plant gene plant genome plant growth potato priority journal protein expression protein metabolism protein synthesis inhibition RCA gene reverse transcription polymerase chain reaction senescence sprout sprouting transcript profiling upregulation biosynthesis darkness gene expression regulation genetics growth, development and aging photosynthesis plant tuber seedling transcription initiation Cysteine Proteinase Inhibitors Darkness Gene Expression Regulation, Plant Light Photosynthesis Plant Proteins Plant Tubers Seedlings Solanum tuberosum Transcriptional Activation
description	Potato (Solanum tuberosum L.) tubers are an excellent staple food due to its high nutritional value. When the tuber reaches physiological competence, sprouting proceeds accompanied by changes at mRNA and protein levels. Potato tubers become a source of carbon and energy until sprouts are capable of independent growth. Transcript profiling of sprouts grown under continuous light or dark conditions was performed using the TIGR 10K EST Solanaceae microarray. The profiles analyzed show a core of highly expressed transcripts that are associated to the reactivation of growth. Under light conditions, the photosynthetic machinery was fully activated; the highest up-regulation was observed for the Rubisco activase (RCA), the glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the Photosystem II 22 kDa protein (CP22) genes, among others. On the other hand, sprouts exposed to continuous darkness elongate longer, and after extended darkness, synthesis of chloroplast components was repressed, the expression of proteases was reduced while genes encoding cysteine protease inhibitors (CPIs) and metallocarboxypeptidase inhibitors (MPIs) were strongly induced. Northern blot and RT-PCR analysis confirmed that MPI levels correlated with the length of the dark period; however, CPI expression was strong only after longer periods of darkness, suggesting a feedback loop (regulation mechanism) in response to dark-induced senescence. Prevention of cysteine protease activity in etiolated sprouts exposed to extended darkness could delay senescence until they emerge to light. © 2016, Springer-Verlag Berlin Heidelberg.
format	JOUR
author	Grandellis, C. Giammaria, V. Fantino, E. Cerrudo, I. Bachmann, S. Santin, F. Ulloa, R.M.
author_facet	Grandellis, C. Giammaria, V. Fantino, E. Cerrudo, I. Bachmann, S. Santin, F. Ulloa, R.M.
author_sort	Grandellis, C.
title	Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness
title_short	Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness
title_full	Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness
title_fullStr	Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness
title_full_unstemmed	Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness
title_sort	transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness
url	http://hdl.handle.net/20.500.12110/paper_1438793X_v16_n4_p399_Grandellis
work_keys_str_mv	AT grandellisc transcriptprofilingrevealsthatcysteineproteaseinhibitorsareupregulatedintubersproutsafterextendeddarkness AT giammariav transcriptprofilingrevealsthatcysteineproteaseinhibitorsareupregulatedintubersproutsafterextendeddarkness AT fantinoe transcriptprofilingrevealsthatcysteineproteaseinhibitorsareupregulatedintubersproutsafterextendeddarkness AT cerrudoi transcriptprofilingrevealsthatcysteineproteaseinhibitorsareupregulatedintubersproutsafterextendeddarkness AT bachmanns transcriptprofilingrevealsthatcysteineproteaseinhibitorsareupregulatedintubersproutsafterextendeddarkness AT santinf transcriptprofilingrevealsthatcysteineproteaseinhibitorsareupregulatedintubersproutsafterextendeddarkness AT ulloarm transcriptprofilingrevealsthatcysteineproteaseinhibitorsareupregulatedintubersproutsafterextendeddarkness
_version_	1807321368390270976

Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness

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