Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes
Xanthomonas citri ssp. citri (X. citri) is the causal agent of Asiatic citrus canker, a disease that seriously affects most commercially important Citrus species worldwide. We have identified previously a natural variant, X. citri AT, that triggers a host-specific defence response in Citrus limon. H...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_14646722_v18_n9_p1267_Roeschlin |
| Aporte de: |
| id |
todo:paper_14646722_v18_n9_p1267_Roeschlin |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_14646722_v18_n9_p1267_Roeschlin2023-10-03T16:17:17Z Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes Roeschlin, R.A. Favaro, M.A. Chiesa, M.A. Alemano, S. Vojnov, A.A. Castagnaro, A.P. Filippone, M.P. Gmitter, F.G., jr. Gadea, J. Marano, M.R. autophagy biofilm formation biological control citrus canker resistance hypersensitive response salicylic acid secondary metabolites Xanthomonas citri ssp. citri (X. citri) is the causal agent of Asiatic citrus canker, a disease that seriously affects most commercially important Citrus species worldwide. We have identified previously a natural variant, X. citri AT, that triggers a host-specific defence response in Citrus limon. However, the mechanisms involved in this canker disease resistance are unknown. In this work, the defence response induced by X. citri AT was assessed by transcriptomic, physiological and ultrastructural analyses, and the effects on bacterial biofilm formation were monitored in parallel. We show that X. citri AT triggers a hypersensitive response associated with the interference of biofilm development and arrest of bacterial growth in C. limon. This plant response involves an extensive transcriptional reprogramming, setting in motion cell wall reinforcement, the oxidative burst and the accumulation of salicylic acid (SA) and phenolic compounds. Ultrastructural analyses revealed subcellular changes involving the activation of autophagy-associated vacuolar processes. Our findings show the activation of SA-dependent defence in response to X. citri AT and suggest a coordinated regulation between the SA and flavonoid pathways, which is associated with autophagy mechanisms that control pathogen invasion in C. limon. Furthermore, this defence response protects C. limon plants from disease on subsequent challenges by pathogenic X. citri. This knowledge will allow the rational exploitation of the plant immune system as a biotechnological approach for the management of the disease. © 2016 BSPP AND JOHN WILEY & SONS LTD. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14646722_v18_n9_p1267_Roeschlin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
autophagy biofilm formation biological control citrus canker resistance hypersensitive response salicylic acid secondary metabolites |
| spellingShingle |
autophagy biofilm formation biological control citrus canker resistance hypersensitive response salicylic acid secondary metabolites Roeschlin, R.A. Favaro, M.A. Chiesa, M.A. Alemano, S. Vojnov, A.A. Castagnaro, A.P. Filippone, M.P. Gmitter, F.G., jr. Gadea, J. Marano, M.R. Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes |
| topic_facet |
autophagy biofilm formation biological control citrus canker resistance hypersensitive response salicylic acid secondary metabolites |
| description |
Xanthomonas citri ssp. citri (X. citri) is the causal agent of Asiatic citrus canker, a disease that seriously affects most commercially important Citrus species worldwide. We have identified previously a natural variant, X. citri AT, that triggers a host-specific defence response in Citrus limon. However, the mechanisms involved in this canker disease resistance are unknown. In this work, the defence response induced by X. citri AT was assessed by transcriptomic, physiological and ultrastructural analyses, and the effects on bacterial biofilm formation were monitored in parallel. We show that X. citri AT triggers a hypersensitive response associated with the interference of biofilm development and arrest of bacterial growth in C. limon. This plant response involves an extensive transcriptional reprogramming, setting in motion cell wall reinforcement, the oxidative burst and the accumulation of salicylic acid (SA) and phenolic compounds. Ultrastructural analyses revealed subcellular changes involving the activation of autophagy-associated vacuolar processes. Our findings show the activation of SA-dependent defence in response to X. citri AT and suggest a coordinated regulation between the SA and flavonoid pathways, which is associated with autophagy mechanisms that control pathogen invasion in C. limon. Furthermore, this defence response protects C. limon plants from disease on subsequent challenges by pathogenic X. citri. This knowledge will allow the rational exploitation of the plant immune system as a biotechnological approach for the management of the disease. © 2016 BSPP AND JOHN WILEY & SONS LTD. |
| format |
JOUR |
| author |
Roeschlin, R.A. Favaro, M.A. Chiesa, M.A. Alemano, S. Vojnov, A.A. Castagnaro, A.P. Filippone, M.P. Gmitter, F.G., jr. Gadea, J. Marano, M.R. |
| author_facet |
Roeschlin, R.A. Favaro, M.A. Chiesa, M.A. Alemano, S. Vojnov, A.A. Castagnaro, A.P. Filippone, M.P. Gmitter, F.G., jr. Gadea, J. Marano, M.R. |
| author_sort |
Roeschlin, R.A. |
| title |
Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes |
| title_short |
Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes |
| title_full |
Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes |
| title_fullStr |
Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes |
| title_full_unstemmed |
Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes |
| title_sort |
resistance to citrus canker induced by a variant of xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes |
| url |
http://hdl.handle.net/20.500.12110/paper_14646722_v18_n9_p1267_Roeschlin |
| work_keys_str_mv |
AT roeschlinra resistancetocitruscankerinducedbyavariantofxanthomonascitrisspcitriisassociatedwithahypersensitivecelldeathresponseinvolvingautophagyassociatedvacuolarprocesses AT favaroma resistancetocitruscankerinducedbyavariantofxanthomonascitrisspcitriisassociatedwithahypersensitivecelldeathresponseinvolvingautophagyassociatedvacuolarprocesses AT chiesama resistancetocitruscankerinducedbyavariantofxanthomonascitrisspcitriisassociatedwithahypersensitivecelldeathresponseinvolvingautophagyassociatedvacuolarprocesses AT alemanos resistancetocitruscankerinducedbyavariantofxanthomonascitrisspcitriisassociatedwithahypersensitivecelldeathresponseinvolvingautophagyassociatedvacuolarprocesses AT vojnovaa resistancetocitruscankerinducedbyavariantofxanthomonascitrisspcitriisassociatedwithahypersensitivecelldeathresponseinvolvingautophagyassociatedvacuolarprocesses AT castagnaroap resistancetocitruscankerinducedbyavariantofxanthomonascitrisspcitriisassociatedwithahypersensitivecelldeathresponseinvolvingautophagyassociatedvacuolarprocesses AT filipponemp resistancetocitruscankerinducedbyavariantofxanthomonascitrisspcitriisassociatedwithahypersensitivecelldeathresponseinvolvingautophagyassociatedvacuolarprocesses AT gmitterfgjr resistancetocitruscankerinducedbyavariantofxanthomonascitrisspcitriisassociatedwithahypersensitivecelldeathresponseinvolvingautophagyassociatedvacuolarprocesses AT gadeaj resistancetocitruscankerinducedbyavariantofxanthomonascitrisspcitriisassociatedwithahypersensitivecelldeathresponseinvolvingautophagyassociatedvacuolarprocesses AT maranomr resistancetocitruscankerinducedbyavariantofxanthomonascitrisspcitriisassociatedwithahypersensitivecelldeathresponseinvolvingautophagyassociatedvacuolarprocesses |
| _version_ |
1807317008090398720 |