Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
We have previously observed expression of prostaglandin-endoperoxide synthase 2 (PTGS2), the key enzyme in the biosynthesis of prostaglandins (PGs), in reproductively active Syrian hamster Leydig cells, and reported an inhibitory role of PGF 2α on hamster testicular steroidogenesis. In this study, w...
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todo:paper_14701626_v138_n1_p163_Matzkin2023-10-03T16:17:36Z Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells Matzkin, M.E. Gonzalez-Calvar, S.I. Mayerhofer, A. Calandra, R.S. Frungieri, M.B. 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene androgen receptor antiandrogen bicalutamide luteinizing hormone mitogen activated protein kinase 1 mitogen activated protein kinase 3 prostaglandin endoperoxide synthase 2 prostaglandin F2 alpha prostaglandin synthase testosterone unclassified drug animal cell animal experiment animal tissue article controlled study enzyme activation hormonal regulation Leydig cell male nonhuman photoperiodicity priority journal prostaglandin synthesis protein expression protein induction protein phosphorylation protein synthesis sexual development spermatogenesis steroidogenesis Syrian hamster Androgen Antagonists Animals Cells, Cultured Chorionic Gonadotropin Cricetinae Cyclooxygenase 2 Dinoprost Enzyme Induction Leydig Cells Luteinizing Hormone Male Mesocricetus Mitogen-Activated Protein Kinases Phosphorylation Photoperiod Protein Kinase Inhibitors Receptors, Androgen Sexual Development Signal Transduction Testosterone Up-Regulation Cricetinae Mesocricetus auratus We have previously observed expression of prostaglandin-endoperoxide synthase 2 (PTGS2), the key enzyme in the biosynthesis of prostaglandins (PGs), in reproductively active Syrian hamster Leydig cells, and reported an inhibitory role of PGF 2α on hamster testicular steroidogenesis. In this study, we further investigated PTGS2 expression in hamster Leydig cells during sexual development and photoperiodic gonadal regression. Since PTGS2 is mostly expressed in pubertal and reproductively active adult hamsters with high circulating levels of LH and androgens, we studied the role of these hormones in the regulation/maintenance of testicular PTGS2/PGF 2α. In active hamster Leydig cells, LH/hCG and testosterone induced PTGS2 and PGF 2α production, and their actions were abolished by the antiandrogen bicalutamide (Bi). These results indicate that LH does not exert a direct effect on PG synthesis. Testosterone also stimulated phosphorylation of the mitogen-activated protein kinase isoforms 3/1 (MAPK3/1) within minutes and hours, but the testosterone metabolite dihydrotestosterone had no effect on PTGS2 and MAPK3/1. Because Bi and U0126, an inhibitor of the MAP kinase kinases 1 and 2 (MAP2K1/2), abolished testosterone actions on MAPK3/1 and PTGS2, our studies suggest that testosterone directly induces PTGS2/PGF 2α in hamster Leydig cells via androgen receptors and a non-classical mechanism that involves MAPK3/1 activation. Since PGF 2α inhibits testosterone production, it might imply the existence of a regulatory loop that is setting a brake on steroidogenesis. Thus, the androgen environment might be crucial for the regulation of testicular PG production at least during sexual development and photoperiodic variations in hamsters. © 2009 Society for Reproduction and Fertility. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14701626_v138_n1_p163_Matzkin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene androgen receptor antiandrogen bicalutamide luteinizing hormone mitogen activated protein kinase 1 mitogen activated protein kinase 3 prostaglandin endoperoxide synthase 2 prostaglandin F2 alpha prostaglandin synthase testosterone unclassified drug animal cell animal experiment animal tissue article controlled study enzyme activation hormonal regulation Leydig cell male nonhuman photoperiodicity priority journal prostaglandin synthesis protein expression protein induction protein phosphorylation protein synthesis sexual development spermatogenesis steroidogenesis Syrian hamster Androgen Antagonists Animals Cells, Cultured Chorionic Gonadotropin Cricetinae Cyclooxygenase 2 Dinoprost Enzyme Induction Leydig Cells Luteinizing Hormone Male Mesocricetus Mitogen-Activated Protein Kinases Phosphorylation Photoperiod Protein Kinase Inhibitors Receptors, Androgen Sexual Development Signal Transduction Testosterone Up-Regulation Cricetinae Mesocricetus auratus |
| spellingShingle |
1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene androgen receptor antiandrogen bicalutamide luteinizing hormone mitogen activated protein kinase 1 mitogen activated protein kinase 3 prostaglandin endoperoxide synthase 2 prostaglandin F2 alpha prostaglandin synthase testosterone unclassified drug animal cell animal experiment animal tissue article controlled study enzyme activation hormonal regulation Leydig cell male nonhuman photoperiodicity priority journal prostaglandin synthesis protein expression protein induction protein phosphorylation protein synthesis sexual development spermatogenesis steroidogenesis Syrian hamster Androgen Antagonists Animals Cells, Cultured Chorionic Gonadotropin Cricetinae Cyclooxygenase 2 Dinoprost Enzyme Induction Leydig Cells Luteinizing Hormone Male Mesocricetus Mitogen-Activated Protein Kinases Phosphorylation Photoperiod Protein Kinase Inhibitors Receptors, Androgen Sexual Development Signal Transduction Testosterone Up-Regulation Cricetinae Mesocricetus auratus Matzkin, M.E. Gonzalez-Calvar, S.I. Mayerhofer, A. Calandra, R.S. Frungieri, M.B. Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells |
| topic_facet |
1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene androgen receptor antiandrogen bicalutamide luteinizing hormone mitogen activated protein kinase 1 mitogen activated protein kinase 3 prostaglandin endoperoxide synthase 2 prostaglandin F2 alpha prostaglandin synthase testosterone unclassified drug animal cell animal experiment animal tissue article controlled study enzyme activation hormonal regulation Leydig cell male nonhuman photoperiodicity priority journal prostaglandin synthesis protein expression protein induction protein phosphorylation protein synthesis sexual development spermatogenesis steroidogenesis Syrian hamster Androgen Antagonists Animals Cells, Cultured Chorionic Gonadotropin Cricetinae Cyclooxygenase 2 Dinoprost Enzyme Induction Leydig Cells Luteinizing Hormone Male Mesocricetus Mitogen-Activated Protein Kinases Phosphorylation Photoperiod Protein Kinase Inhibitors Receptors, Androgen Sexual Development Signal Transduction Testosterone Up-Regulation Cricetinae Mesocricetus auratus |
| description |
We have previously observed expression of prostaglandin-endoperoxide synthase 2 (PTGS2), the key enzyme in the biosynthesis of prostaglandins (PGs), in reproductively active Syrian hamster Leydig cells, and reported an inhibitory role of PGF 2α on hamster testicular steroidogenesis. In this study, we further investigated PTGS2 expression in hamster Leydig cells during sexual development and photoperiodic gonadal regression. Since PTGS2 is mostly expressed in pubertal and reproductively active adult hamsters with high circulating levels of LH and androgens, we studied the role of these hormones in the regulation/maintenance of testicular PTGS2/PGF 2α. In active hamster Leydig cells, LH/hCG and testosterone induced PTGS2 and PGF 2α production, and their actions were abolished by the antiandrogen bicalutamide (Bi). These results indicate that LH does not exert a direct effect on PG synthesis. Testosterone also stimulated phosphorylation of the mitogen-activated protein kinase isoforms 3/1 (MAPK3/1) within minutes and hours, but the testosterone metabolite dihydrotestosterone had no effect on PTGS2 and MAPK3/1. Because Bi and U0126, an inhibitor of the MAP kinase kinases 1 and 2 (MAP2K1/2), abolished testosterone actions on MAPK3/1 and PTGS2, our studies suggest that testosterone directly induces PTGS2/PGF 2α in hamster Leydig cells via androgen receptors and a non-classical mechanism that involves MAPK3/1 activation. Since PGF 2α inhibits testosterone production, it might imply the existence of a regulatory loop that is setting a brake on steroidogenesis. Thus, the androgen environment might be crucial for the regulation of testicular PG production at least during sexual development and photoperiodic variations in hamsters. © 2009 Society for Reproduction and Fertility. |
| format |
JOUR |
| author |
Matzkin, M.E. Gonzalez-Calvar, S.I. Mayerhofer, A. Calandra, R.S. Frungieri, M.B. |
| author_facet |
Matzkin, M.E. Gonzalez-Calvar, S.I. Mayerhofer, A. Calandra, R.S. Frungieri, M.B. |
| author_sort |
Matzkin, M.E. |
| title |
Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells |
| title_short |
Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells |
| title_full |
Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells |
| title_fullStr |
Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells |
| title_full_unstemmed |
Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells |
| title_sort |
testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin f 2α production in hamster leydig cells |
| url |
http://hdl.handle.net/20.500.12110/paper_14701626_v138_n1_p163_Matzkin |
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1807320410943913984 |