Determination of the molecular size of BSA by fluorescence anisotropy

This work describes a laboratory experiment to illustrate the usefulness of fluorescence anisotropy in the field of biophysics. Fluorescence anisotropy of dansyl-labeled BSA was determined in media of increasing glycerol concentrations. The Perrin equation was fitted to the experimental data, obtain...

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Detalles Bibliográficos
Autores principales: Flecha, F.L.G., Levi, V.
Formato: JOUR
Materias:
BSA
Fluorescence anisotropy
Molecular size
glycerol
protein
protein BSA
unclassified drug
anisotropy
article
biophysics
data analysis
fluorescence analysis
molecular probe
molecular size
university student
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14708175_v31_n5_p319_Flecha
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_14708175_v31_n5_p319_Flecha
record_format dspace
spelling todo:paper_14708175_v31_n5_p319_Flecha2023-10-03T16:17:41Z Determination of the molecular size of BSA by fluorescence anisotropy Flecha, F.L.G. Levi, V. BSA Fluorescence anisotropy Molecular size glycerol protein protein BSA unclassified drug anisotropy article biophysics data analysis fluorescence analysis molecular probe molecular size university student This work describes a laboratory experiment to illustrate the usefulness of fluorescence anisotropy in the field of biophysics. Fluorescence anisotropy of dansyl-labeled BSA was determined in media of increasing glycerol concentrations. The Perrin equation was fitted to the experimental data, obtaining the molecular volume of the protein. The simplicity of the experiment and data analysis helped the students to focus on the relationship between probe anisotropy and rotational diffusion. Additionally, this laboratory exercise has the advantage of using a protein and a probe that are inexpensive and very common in many laboratories. © 2003 by The International Union of Biochemistry and Molecular Biology. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14708175_v31_n5_p319_Flecha
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic BSA
Fluorescence anisotropy
Molecular size
glycerol
protein
protein BSA
unclassified drug
anisotropy
article
biophysics
data analysis
fluorescence analysis
molecular probe
molecular size
university student
spellingShingle BSA
Fluorescence anisotropy
Molecular size
glycerol
protein
protein BSA
unclassified drug
anisotropy
article
biophysics
data analysis
fluorescence analysis
molecular probe
molecular size
university student
Flecha, F.L.G.
Levi, V.
Determination of the molecular size of BSA by fluorescence anisotropy
topic_facet BSA
Fluorescence anisotropy
Molecular size
glycerol
protein
protein BSA
unclassified drug
anisotropy
article
biophysics
data analysis
fluorescence analysis
molecular probe
molecular size
university student
description This work describes a laboratory experiment to illustrate the usefulness of fluorescence anisotropy in the field of biophysics. Fluorescence anisotropy of dansyl-labeled BSA was determined in media of increasing glycerol concentrations. The Perrin equation was fitted to the experimental data, obtaining the molecular volume of the protein. The simplicity of the experiment and data analysis helped the students to focus on the relationship between probe anisotropy and rotational diffusion. Additionally, this laboratory exercise has the advantage of using a protein and a probe that are inexpensive and very common in many laboratories. © 2003 by The International Union of Biochemistry and Molecular Biology.
format JOUR
author Flecha, F.L.G.
Levi, V.
author_facet Flecha, F.L.G.
Levi, V.
author_sort Flecha, F.L.G.
title Determination of the molecular size of BSA by fluorescence anisotropy
title_short Determination of the molecular size of BSA by fluorescence anisotropy
title_full Determination of the molecular size of BSA by fluorescence anisotropy
title_fullStr Determination of the molecular size of BSA by fluorescence anisotropy
title_full_unstemmed Determination of the molecular size of BSA by fluorescence anisotropy
title_sort determination of the molecular size of bsa by fluorescence anisotropy
url http://hdl.handle.net/20.500.12110/paper_14708175_v31_n5_p319_Flecha
work_keys_str_mv AT flechaflg determinationofthemolecularsizeofbsabyfluorescenceanisotropy
AT leviv determinationofthemolecularsizeofbsabyfluorescenceanisotropy
_version_ 1807320864092323840

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