Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories
Fast and easy-to-do methods capable to generate metabolite profile of plant single cells and tissues can be quite beneficial for analyzing biochemical and physiological status of growing crops in plant growth factories. Here we report the applicability of Pressure Probe, which is routinely used for...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_14746670_v44_n1PART1_p1791_Nonami |
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todo:paper_14746670_v44_n1PART1_p1791_Nonami2023-10-03T16:18:12Z Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories Nonami, H. Gholipour, Y. Erra-Balsells, R. Carbon nanotube In situ profiling Inorganic nanoparticle MALDI MS Pressure probe Tulip Biomolecules Carbon nanotubes Cells Cytology Histology Mass spectrometry Metabolites Nanoparticles Physiology Plant cell culture Plant life extension Potential flow Probes Reliability analysis Sugars Tissue Yarn Inorganic nanoparticle MALDI-mass spectrometry MALDI-MS Metabolite extraction Metabolite profiles Physiological status Pressure probes Tulip Tissue engineering Fast and easy-to-do methods capable to generate metabolite profile of plant single cells and tissues can be quite beneficial for analyzing biochemical and physiological status of growing crops in plant growth factories. Here we report the applicability of Pressure Probe, which is routinely used for pressure probing of living single cells, for microsampling of plant cells. The picoliter sample of cytoplasm was then analyzed for characterizing metabolite content by MALDI mass spectrometry (MS). This powerful technique could make molecular profile of the cell samples by detecting very small amount of metabolites by using our selected matrixes, after verification of detected signals by MS/MS analysis. Concurrent to single cell sampling and mass spectrometry, the plant tissues, from which single cells were analyzed, were directly profiled by MALDI MS after deposition of our novel nanoparticle matrixes on the dried surface of tissue. MALDI MS showed to be able to detect and verify metabolites, particularly sugars, directly from the surface of plant tissues. Interestingly, metabolites, particularly neutral sugars, detected in cell microsamples were comparable to those detected on the tissue. Accordingly, the single cell and tissue analysis can be concurrently carried out to create metabolite profile, an instant picture of the physiology in cell and tissue levels respectively, of plants during growth without need to common metabolite extraction and purification processes, with minimum preparation and high accuracy and reliability. © 2011 IFAC. CONF info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14746670_v44_n1PART1_p1791_Nonami |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Carbon nanotube In situ profiling Inorganic nanoparticle MALDI MS Pressure probe Tulip Biomolecules Carbon nanotubes Cells Cytology Histology Mass spectrometry Metabolites Nanoparticles Physiology Plant cell culture Plant life extension Potential flow Probes Reliability analysis Sugars Tissue Yarn Inorganic nanoparticle MALDI-mass spectrometry MALDI-MS Metabolite extraction Metabolite profiles Physiological status Pressure probes Tulip Tissue engineering |
| spellingShingle |
Carbon nanotube In situ profiling Inorganic nanoparticle MALDI MS Pressure probe Tulip Biomolecules Carbon nanotubes Cells Cytology Histology Mass spectrometry Metabolites Nanoparticles Physiology Plant cell culture Plant life extension Potential flow Probes Reliability analysis Sugars Tissue Yarn Inorganic nanoparticle MALDI-mass spectrometry MALDI-MS Metabolite extraction Metabolite profiles Physiological status Pressure probes Tulip Tissue engineering Nonami, H. Gholipour, Y. Erra-Balsells, R. Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories |
| topic_facet |
Carbon nanotube In situ profiling Inorganic nanoparticle MALDI MS Pressure probe Tulip Biomolecules Carbon nanotubes Cells Cytology Histology Mass spectrometry Metabolites Nanoparticles Physiology Plant cell culture Plant life extension Potential flow Probes Reliability analysis Sugars Tissue Yarn Inorganic nanoparticle MALDI-mass spectrometry MALDI-MS Metabolite extraction Metabolite profiles Physiological status Pressure probes Tulip Tissue engineering |
| description |
Fast and easy-to-do methods capable to generate metabolite profile of plant single cells and tissues can be quite beneficial for analyzing biochemical and physiological status of growing crops in plant growth factories. Here we report the applicability of Pressure Probe, which is routinely used for pressure probing of living single cells, for microsampling of plant cells. The picoliter sample of cytoplasm was then analyzed for characterizing metabolite content by MALDI mass spectrometry (MS). This powerful technique could make molecular profile of the cell samples by detecting very small amount of metabolites by using our selected matrixes, after verification of detected signals by MS/MS analysis. Concurrent to single cell sampling and mass spectrometry, the plant tissues, from which single cells were analyzed, were directly profiled by MALDI MS after deposition of our novel nanoparticle matrixes on the dried surface of tissue. MALDI MS showed to be able to detect and verify metabolites, particularly sugars, directly from the surface of plant tissues. Interestingly, metabolites, particularly neutral sugars, detected in cell microsamples were comparable to those detected on the tissue. Accordingly, the single cell and tissue analysis can be concurrently carried out to create metabolite profile, an instant picture of the physiology in cell and tissue levels respectively, of plants during growth without need to common metabolite extraction and purification processes, with minimum preparation and high accuracy and reliability. © 2011 IFAC. |
| format |
CONF |
| author |
Nonami, H. Gholipour, Y. Erra-Balsells, R. |
| author_facet |
Nonami, H. Gholipour, Y. Erra-Balsells, R. |
| author_sort |
Nonami, H. |
| title |
Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories |
| title_short |
Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories |
| title_full |
Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories |
| title_fullStr |
Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories |
| title_full_unstemmed |
Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories |
| title_sort |
plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories |
| url |
http://hdl.handle.net/20.500.12110/paper_14746670_v44_n1PART1_p1791_Nonami |
| work_keys_str_mv |
AT nonamih plantcellmeasurementswithmassspectrometryforspeakingcellapproachinplantgrowthfactories AT gholipoury plantcellmeasurementswithmassspectrometryforspeakingcellapproachinplantgrowthfactories AT errabalsellsr plantcellmeasurementswithmassspectrometryforspeakingcellapproachinplantgrowthfactories |
| _version_ |
1807317719494688768 |