Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring...

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Detalles Bibliográficos
Autores principales: Liras, M., Simoncelli, S., Rivas-Aravena, A., García, O., Scaiano, J.C., Alarcon, E.I., Aspée, A.
Formato: JOUR
Materias:
Amides
Cell culture
Cytology
Fibroblasts
Fluorescence
Fluorescence microscopy
Cellular redox
Fluorescence intensities
Human fibroblast
Nitroxides
Probes
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
amide
boron derivative
fluorescent dye
hydrogen peroxide
nitrogen oxide
cell culture
chemistry
fibroblast
fluorescence microscopy
human
metabolism
oxidation reduction reaction
synthesis
Boron Compounds
Cells, Cultured
Fluorescent Dyes
Humans
Hydrogen Peroxide
Microscopy, Fluorescence
Nitrogen Oxides
Oxidation-Reduction
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14770520_v14_n17_p4023_Liras
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered. © 2016 The Royal Society of Chemistry.

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