Update on primary HIV-1 resistance in Argentina: Emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients

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Here we present a survey including 52 drug-naive recently HIV-1-infected subjects from Buenos Aires City and province (79%) and 3 other regions in Argentina (21%). Recent infections were established from previous negative serology (32/52), indeterminate Western blot (12/52), or acute retroviral synd...

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Autores principales:	Petroni, A., Deluchi, G., Pryluka, D., Rotryng, F., Bortolozzi, R., Lopardo, G., Bouzas, M.B., Zapiola, I., Garone, D., Rodríguez, C., Chiocconi, E., Lázaro, M.E., Murano, F., Maranzana, A., Oliva, S.M., Aparicio, M., Beltrán, M., Benetucci, J.A.
Formato:	JOUR
Materias:	Argentina B/F recombinant viruses HIV-1 primary resistance K103N L89M M41L proteinase recombinant enzyme RNA directed DNA polymerase RNA directed DNA polymerase inhibitor adult amino acid sequence amino acid substitution controlled study exposure female gene mutation genotype health survey high risk population human Human immunodeficiency virus 1 Human immunodeficiency virus infection major clinical study male nucleotide sequence phylogeny priority journal review serology virus resistance Western blotting Base Sequence DNA Primers Female HIV Infections HIV-1 Humans Male Mutation Phylogeny Polymorphism, Genetic Recombination, Genetic Reverse Transcriptase Inhibitors Reverse Transcriptase Polymerase Chain Reaction
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15254135_v42_n4_p506_Petroni
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_15254135_v42_n4_p506_Petroni
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spelling	todo:paper_15254135_v42_n4_p506_Petroni2023-10-03T16:21:01Z Update on primary HIV-1 resistance in Argentina: Emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients Petroni, A. Deluchi, G. Pryluka, D. Rotryng, F. Bortolozzi, R. Lopardo, G. Bouzas, M.B. Zapiola, I. Garone, D. Rodríguez, C. Chiocconi, E. Lázaro, M.E. Murano, F. Maranzana, A. Oliva, S.M. Aparicio, M. Beltrán, M. Benetucci, J.A. Argentina B/F recombinant viruses HIV-1 primary resistance K103N L89M M41L proteinase recombinant enzyme RNA directed DNA polymerase RNA directed DNA polymerase inhibitor adult amino acid sequence amino acid substitution Argentina controlled study exposure female gene mutation genotype health survey high risk population human Human immunodeficiency virus 1 Human immunodeficiency virus infection major clinical study male nucleotide sequence phylogeny priority journal review serology virus resistance Western blotting Argentina Base Sequence DNA Primers Female HIV Infections HIV-1 Humans Male Mutation Phylogeny Polymorphism, Genetic Recombination, Genetic Reverse Transcriptase Inhibitors Reverse Transcriptase Polymerase Chain Reaction Here we present a survey including 52 drug-naive recently HIV-1-infected subjects from Buenos Aires City and province (79%) and 3 other regions in Argentina (21%). Recent infections were established from previous negative serology (32/52), indeterminate Western blot (12/52), or acute retroviral syndrome after high-risk HIV exposure (8/52) within 9 months before genotyping (median time, 4.2 months). Genotyping was performed from plasma by sequencing both protease and reverse transcriptase. Phylogenetic analysis combined with bootscanning resulted in 21 subtype B sequences and 31 B/F recombinants (RecBF). On protease, minor resistance-related mutations were found in both subtype B and RecBF with low frequencies. The substitution L89M, recently suggested as a resistance-related mutation in some subtype F viruses, was observed in 1 RecBF. On reverse transcriptase, major resistance-related mutations were found in 4 of 52 (7.7%) patients from different health centers: M41L (subtype B) and K103N±P225H (1 RecBF and 2 subtype B). The greater than 5% resistance threshold found indicates a need for sentinel resistance surveillances calling for an update in the current resistance testing guidelines in Argentina. Copyright © 2006 by Lippincott Williams & Wilkins. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15254135_v42_n4_p506_Petroni
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Argentina B/F recombinant viruses HIV-1 primary resistance K103N L89M M41L proteinase recombinant enzyme RNA directed DNA polymerase RNA directed DNA polymerase inhibitor adult amino acid sequence amino acid substitution Argentina controlled study exposure female gene mutation genotype health survey high risk population human Human immunodeficiency virus 1 Human immunodeficiency virus infection major clinical study male nucleotide sequence phylogeny priority journal review serology virus resistance Western blotting Argentina Base Sequence DNA Primers Female HIV Infections HIV-1 Humans Male Mutation Phylogeny Polymorphism, Genetic Recombination, Genetic Reverse Transcriptase Inhibitors Reverse Transcriptase Polymerase Chain Reaction
spellingShingle	Argentina B/F recombinant viruses HIV-1 primary resistance K103N L89M M41L proteinase recombinant enzyme RNA directed DNA polymerase RNA directed DNA polymerase inhibitor adult amino acid sequence amino acid substitution Argentina controlled study exposure female gene mutation genotype health survey high risk population human Human immunodeficiency virus 1 Human immunodeficiency virus infection major clinical study male nucleotide sequence phylogeny priority journal review serology virus resistance Western blotting Argentina Base Sequence DNA Primers Female HIV Infections HIV-1 Humans Male Mutation Phylogeny Polymorphism, Genetic Recombination, Genetic Reverse Transcriptase Inhibitors Reverse Transcriptase Polymerase Chain Reaction Petroni, A. Deluchi, G. Pryluka, D. Rotryng, F. Bortolozzi, R. Lopardo, G. Bouzas, M.B. Zapiola, I. Garone, D. Rodríguez, C. Chiocconi, E. Lázaro, M.E. Murano, F. Maranzana, A. Oliva, S.M. Aparicio, M. Beltrán, M. Benetucci, J.A. Update on primary HIV-1 resistance in Argentina: Emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients
topic_facet	Argentina B/F recombinant viruses HIV-1 primary resistance K103N L89M M41L proteinase recombinant enzyme RNA directed DNA polymerase RNA directed DNA polymerase inhibitor adult amino acid sequence amino acid substitution Argentina controlled study exposure female gene mutation genotype health survey high risk population human Human immunodeficiency virus 1 Human immunodeficiency virus infection major clinical study male nucleotide sequence phylogeny priority journal review serology virus resistance Western blotting Argentina Base Sequence DNA Primers Female HIV Infections HIV-1 Humans Male Mutation Phylogeny Polymorphism, Genetic Recombination, Genetic Reverse Transcriptase Inhibitors Reverse Transcriptase Polymerase Chain Reaction
description	Here we present a survey including 52 drug-naive recently HIV-1-infected subjects from Buenos Aires City and province (79%) and 3 other regions in Argentina (21%). Recent infections were established from previous negative serology (32/52), indeterminate Western blot (12/52), or acute retroviral syndrome after high-risk HIV exposure (8/52) within 9 months before genotyping (median time, 4.2 months). Genotyping was performed from plasma by sequencing both protease and reverse transcriptase. Phylogenetic analysis combined with bootscanning resulted in 21 subtype B sequences and 31 B/F recombinants (RecBF). On protease, minor resistance-related mutations were found in both subtype B and RecBF with low frequencies. The substitution L89M, recently suggested as a resistance-related mutation in some subtype F viruses, was observed in 1 RecBF. On reverse transcriptase, major resistance-related mutations were found in 4 of 52 (7.7%) patients from different health centers: M41L (subtype B) and K103N±P225H (1 RecBF and 2 subtype B). The greater than 5% resistance threshold found indicates a need for sentinel resistance surveillances calling for an update in the current resistance testing guidelines in Argentina. Copyright © 2006 by Lippincott Williams & Wilkins.
format	JOUR
author	Petroni, A. Deluchi, G. Pryluka, D. Rotryng, F. Bortolozzi, R. Lopardo, G. Bouzas, M.B. Zapiola, I. Garone, D. Rodríguez, C. Chiocconi, E. Lázaro, M.E. Murano, F. Maranzana, A. Oliva, S.M. Aparicio, M. Beltrán, M. Benetucci, J.A.
author_facet	Petroni, A. Deluchi, G. Pryluka, D. Rotryng, F. Bortolozzi, R. Lopardo, G. Bouzas, M.B. Zapiola, I. Garone, D. Rodríguez, C. Chiocconi, E. Lázaro, M.E. Murano, F. Maranzana, A. Oliva, S.M. Aparicio, M. Beltrán, M. Benetucci, J.A.
author_sort	Petroni, A.
title	Update on primary HIV-1 resistance in Argentina: Emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients
title_short	Update on primary HIV-1 resistance in Argentina: Emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients
title_full	Update on primary HIV-1 resistance in Argentina: Emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients
title_fullStr	Update on primary HIV-1 resistance in Argentina: Emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients
title_full_unstemmed	Update on primary HIV-1 resistance in Argentina: Emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients
title_sort	update on primary hiv-1 resistance in argentina: emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients
url	http://hdl.handle.net/20.500.12110/paper_15254135_v42_n4_p506_Petroni
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Update on primary HIV-1 resistance in Argentina: Emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients

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