Update on primary HIV-1 resistance in Argentina: Emergence of mutations conferring high-level resistance to nonnucleoside reverse transcriptase inhibitors in drug-naive patients

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Here we present a survey including 52 drug-naive recently HIV-1-infected subjects from Buenos Aires City and province (79%) and 3 other regions in Argentina (21%). Recent infections were established from previous negative serology (32/52), indeterminate Western blot (12/52), or acute retroviral synd...

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Autores principales: Petroni, A., Deluchi, G., Pryluka, D., Rotryng, F., Bortolozzi, R., Lopardo, G., Bouzas, M.B., Zapiola, I., Garone, D., Rodríguez, C., Chiocconi, E., Lázaro, M.E., Murano, F., Maranzana, A., Oliva, S.M., Aparicio, M., Beltrán, M., Benetucci, J.A.
Formato: JOUR
Materias:
Argentina
B/F recombinant viruses
HIV-1 primary resistance
K103N
L89M
M41L
proteinase
recombinant enzyme
RNA directed DNA polymerase
RNA directed DNA polymerase inhibitor
adult
amino acid sequence
amino acid substitution
controlled study
exposure
female
gene mutation
genotype
health survey
high risk population
human
Human immunodeficiency virus 1
Human immunodeficiency virus infection
major clinical study
male
nucleotide sequence
phylogeny
priority journal
review
serology
virus resistance
Western blotting
Base Sequence
DNA Primers
Female
HIV Infections
HIV-1
Humans
Male
Mutation
Phylogeny
Polymorphism, Genetic
Recombination, Genetic
Reverse Transcriptase Inhibitors
Reverse Transcriptase Polymerase Chain Reaction
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15254135_v42_n4_p506_Petroni
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Here we present a survey including 52 drug-naive recently HIV-1-infected subjects from Buenos Aires City and province (79%) and 3 other regions in Argentina (21%). Recent infections were established from previous negative serology (32/52), indeterminate Western blot (12/52), or acute retroviral syndrome after high-risk HIV exposure (8/52) within 9 months before genotyping (median time, 4.2 months). Genotyping was performed from plasma by sequencing both protease and reverse transcriptase. Phylogenetic analysis combined with bootscanning resulted in 21 subtype B sequences and 31 B/F recombinants (RecBF). On protease, minor resistance-related mutations were found in both subtype B and RecBF with low frequencies. The substitution L89M, recently suggested as a resistance-related mutation in some subtype F viruses, was observed in 1 RecBF. On reverse transcriptase, major resistance-related mutations were found in 4 of 52 (7.7%) patients from different health centers: M41L (subtype B) and K103N±P225H (1 RecBF and 2 subtype B). The greater than 5% resistance threshold found indicates a need for sentinel resistance surveillances calling for an update in the current resistance testing guidelines in Argentina. Copyright © 2006 by Lippincott Williams & Wilkins.

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