The role of the C-terminal region on the oligomeric state and enzymatic activity of Trypanosoma cruzi hypoxanthine phosphoribosyl transferase

Hypoxanthine phosphoribosyl transferase from Trypanosoma cruzi (TcHPRT) is a critical enzyme for the survival of the parasite. This work demonstrates that the full-length form in solution adopts a stable and enzymatically active tetrameric form, exhibiting large inter-subunit surfaces. Although this...

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Detalles Bibliográficos
Autores principales: Valsecchi, W.M., Cousido-Siah, A., Defelipe, L.A., Mitschler, A., Podjarny, A., Santos, J., Delfino, J.M.
Formato: JOUR
Materias:
Bisphosphonates
Disorder C-terminal region
Enzymatic activity modulation
Proteolysis
Quaternary structure
Reversible oligomerization
Stability
bisphosphonic acid derivative
hypoxanthine phosphoribosyltransferase
oligomer
biopolymer
Article
carboxy terminal sequence
circular dichroism
enzyme activity
enzyme conformation
fluorescence spectroscopy
hydrodynamics
oligomerization
priority journal
protein degradation
protein quaternary structure
protein unfolding
Trypanosoma cruzi
X ray crystallography
amino acid sequence
chemistry
enzymology
metabolism
molecular genetics
ultraviolet spectrophotometry
Amino Acid Sequence
Biopolymers
Circular Dichroism
Hypoxanthine Phosphoribosyltransferase
Molecular Sequence Data
Spectrophotometry, Ultraviolet
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15709639_v1864_n6_p655_Valsecchi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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