HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic
Early detection of toxic events induced by xenobiotics is necessary for a proper assessment of human risk after the exposure to those agents. The aim of this work was to evaluate the cell line HEp-2 as an experimental model to determine the genotoxic effects of sodium arsenate. To this end, we deter...
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todo:paper_16660390_v28_n2_p15_Andrioli2023-10-03T16:29:16Z HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic Andrioli, N.B. Chaufan, G. Coalova, I. Ríos De Molina, M.C. Mudry, M.D. Cytotoxicity Genotoxicity Glutathione HEp-2 cell line arsenate sodium glutathione glutathione transferase Article chromosome aberration concentration (parameters) controlled study cytotoxicity enzyme activity evaluation study experimental model genotoxicity HEp-2 cell line human human cell LC50 lowest observed effect concentration micronucleus micronucleus test MTT assay Early detection of toxic events induced by xenobiotics is necessary for a proper assessment of human risk after the exposure to those agents. The aim of this work was to evaluate the cell line HEp-2 as an experimental model to determine the genotoxic effects of sodium arsenate. To this end, we determined the metabolic activity cells by the MTT test on seven concentrations of arsenate that range from 27 to 135,000 μM, obtaining the median lethal concentration (LC50), the lowest observed effect concentration (LOEC), and the not observed effect concentration (NOEC) of sodium arsenate at 24 h of exposition. According to the cytotoxic response obtained, we evaluated the genotoxic effect of the 27 and 270 μM concentrations by using the micronucleus assay and chromosomal aberrations test. We found a statistically significant increase (p<0.05) in the frequency of micronuclei between control cultures and those exposed to the highest concentration of sodium arsenate. Furthermore, the frequencies of nucleoplasmic bridges and tripolar mitosis were significantly higher in cell cultures exposed to the above concentrations compared to the control cultures (p<0.05). The participation of the glutathione system as response to the arsenate exposition was also analyzed, and a statistically significant increase in the glutathione content was found in those cells exposed to 27 μM of arsenate. The Glutathione S-transferase activity did not increase in the exposed cells compared to control cells, suggesting that the arsenate reduction involved other metabolic pathways in the HEp-2 cells. These results confirm that, under the conditions carried out in this study, sodium arsenate is genotoxic for HEp-2 cells. Therefore, we suggest that this cell line would be a good model for the assessment of the cytotoxic and genotoxic effects of xenobiotics on human cells. © 2017 Sociedad Argentina de Genetica. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16660390_v28_n2_p15_Andrioli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cytotoxicity Genotoxicity Glutathione HEp-2 cell line arsenate sodium glutathione glutathione transferase Article chromosome aberration concentration (parameters) controlled study cytotoxicity enzyme activity evaluation study experimental model genotoxicity HEp-2 cell line human human cell LC50 lowest observed effect concentration micronucleus micronucleus test MTT assay |
| spellingShingle |
Cytotoxicity Genotoxicity Glutathione HEp-2 cell line arsenate sodium glutathione glutathione transferase Article chromosome aberration concentration (parameters) controlled study cytotoxicity enzyme activity evaluation study experimental model genotoxicity HEp-2 cell line human human cell LC50 lowest observed effect concentration micronucleus micronucleus test MTT assay Andrioli, N.B. Chaufan, G. Coalova, I. Ríos De Molina, M.C. Mudry, M.D. HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic |
| topic_facet |
Cytotoxicity Genotoxicity Glutathione HEp-2 cell line arsenate sodium glutathione glutathione transferase Article chromosome aberration concentration (parameters) controlled study cytotoxicity enzyme activity evaluation study experimental model genotoxicity HEp-2 cell line human human cell LC50 lowest observed effect concentration micronucleus micronucleus test MTT assay |
| description |
Early detection of toxic events induced by xenobiotics is necessary for a proper assessment of human risk after the exposure to those agents. The aim of this work was to evaluate the cell line HEp-2 as an experimental model to determine the genotoxic effects of sodium arsenate. To this end, we determined the metabolic activity cells by the MTT test on seven concentrations of arsenate that range from 27 to 135,000 μM, obtaining the median lethal concentration (LC50), the lowest observed effect concentration (LOEC), and the not observed effect concentration (NOEC) of sodium arsenate at 24 h of exposition. According to the cytotoxic response obtained, we evaluated the genotoxic effect of the 27 and 270 μM concentrations by using the micronucleus assay and chromosomal aberrations test. We found a statistically significant increase (p<0.05) in the frequency of micronuclei between control cultures and those exposed to the highest concentration of sodium arsenate. Furthermore, the frequencies of nucleoplasmic bridges and tripolar mitosis were significantly higher in cell cultures exposed to the above concentrations compared to the control cultures (p<0.05). The participation of the glutathione system as response to the arsenate exposition was also analyzed, and a statistically significant increase in the glutathione content was found in those cells exposed to 27 μM of arsenate. The Glutathione S-transferase activity did not increase in the exposed cells compared to control cells, suggesting that the arsenate reduction involved other metabolic pathways in the HEp-2 cells. These results confirm that, under the conditions carried out in this study, sodium arsenate is genotoxic for HEp-2 cells. Therefore, we suggest that this cell line would be a good model for the assessment of the cytotoxic and genotoxic effects of xenobiotics on human cells. © 2017 Sociedad Argentina de Genetica. All rights reserved. |
| format |
JOUR |
| author |
Andrioli, N.B. Chaufan, G. Coalova, I. Ríos De Molina, M.C. Mudry, M.D. |
| author_facet |
Andrioli, N.B. Chaufan, G. Coalova, I. Ríos De Molina, M.C. Mudry, M.D. |
| author_sort |
Andrioli, N.B. |
| title |
HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic |
| title_short |
HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic |
| title_full |
HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic |
| title_fullStr |
HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic |
| title_full_unstemmed |
HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic |
| title_sort |
hep-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic |
| url |
http://hdl.handle.net/20.500.12110/paper_16660390_v28_n2_p15_Andrioli |
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