Toxicity, freezing and cold storage tests of native species semen
In the first part of this study, toxicity tests were performed on the sperm of Macrobrachium acanthurus using four cryoprotectants for periods of 10 and 20 min at concentrations of 10 and 20%. In the second part, cryopreservation was performed by applying the least toxic cryoprotectant, and two free...
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todo:paper_16782305_v43_n3_p334_daCosta2023-10-03T16:29:40Z Toxicity, freezing and cold storage tests of native species semen da Costa, T.V. Oshiro, L.M.Y. de Mattos, L.A. López Greco, L.S. Melo, E.P. Flor, H.R. Caridea Cryopreservation Cryoprotectants Glycerol Methanol Shrimp In the first part of this study, toxicity tests were performed on the sperm of Macrobrachium acanthurus using four cryoprotectants for periods of 10 and 20 min at concentrations of 10 and 20%. In the second part, cryopreservation was performed by applying the least toxic cryoprotectant, and two freezing methods were tested over 24 hours: automated (protocols A and B) and conventional (protocols C and D). Protocol A exhibited a cooling rate of 0.5°C min-1 from -6°C to -32°C; protocol B was similar to A except for the starting temperature, which was room temperature; whereas protocols C and D exhibited a cooling rate of 2 and 10°C min-1, respectively. The third part of the study was conducted to assess the lifespan of the sperm when stored at 5°C, in which sperm viability was evaluated by a semen smear with eosin-nigrosin. The least toxic cryoprotectants were 10 and 20% glycerol, and 10% methanol, and the equilibrium time was 10 minutes. The optimal cooling rate was 2°C min-1 for 10% glycerol, which had a sperm survival rate of 21.8%. Cold storage for up to 3 days is recommended, presenting a sperm survival rate of 35.3%. © 2017, Instytut Technologii Drewna. All Rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16782305_v43_n3_p334_daCosta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Caridea Cryopreservation Cryoprotectants Glycerol Methanol Shrimp |
| spellingShingle |
Caridea Cryopreservation Cryoprotectants Glycerol Methanol Shrimp da Costa, T.V. Oshiro, L.M.Y. de Mattos, L.A. López Greco, L.S. Melo, E.P. Flor, H.R. Toxicity, freezing and cold storage tests of native species semen |
| topic_facet |
Caridea Cryopreservation Cryoprotectants Glycerol Methanol Shrimp |
| description |
In the first part of this study, toxicity tests were performed on the sperm of Macrobrachium acanthurus using four cryoprotectants for periods of 10 and 20 min at concentrations of 10 and 20%. In the second part, cryopreservation was performed by applying the least toxic cryoprotectant, and two freezing methods were tested over 24 hours: automated (protocols A and B) and conventional (protocols C and D). Protocol A exhibited a cooling rate of 0.5°C min-1 from -6°C to -32°C; protocol B was similar to A except for the starting temperature, which was room temperature; whereas protocols C and D exhibited a cooling rate of 2 and 10°C min-1, respectively. The third part of the study was conducted to assess the lifespan of the sperm when stored at 5°C, in which sperm viability was evaluated by a semen smear with eosin-nigrosin. The least toxic cryoprotectants were 10 and 20% glycerol, and 10% methanol, and the equilibrium time was 10 minutes. The optimal cooling rate was 2°C min-1 for 10% glycerol, which had a sperm survival rate of 21.8%. Cold storage for up to 3 days is recommended, presenting a sperm survival rate of 35.3%. © 2017, Instytut Technologii Drewna. All Rights reserved. |
| format |
JOUR |
| author |
da Costa, T.V. Oshiro, L.M.Y. de Mattos, L.A. López Greco, L.S. Melo, E.P. Flor, H.R. |
| author_facet |
da Costa, T.V. Oshiro, L.M.Y. de Mattos, L.A. López Greco, L.S. Melo, E.P. Flor, H.R. |
| author_sort |
da Costa, T.V. |
| title |
Toxicity, freezing and cold storage tests of native species semen |
| title_short |
Toxicity, freezing and cold storage tests of native species semen |
| title_full |
Toxicity, freezing and cold storage tests of native species semen |
| title_fullStr |
Toxicity, freezing and cold storage tests of native species semen |
| title_full_unstemmed |
Toxicity, freezing and cold storage tests of native species semen |
| title_sort |
toxicity, freezing and cold storage tests of native species semen |
| url |
http://hdl.handle.net/20.500.12110/paper_16782305_v43_n3_p334_daCosta |
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