Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells

Aim: Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages. Materials & methods: CPNs were developed employing 9,9-dioctylfluorene-alt-benzothiadiazo...

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Autores principales: Ibarra, L.E., Porcal, G.V., Macor, L.P., Ponzio, R.A., Spada, R.M., Lorente, C., Chesta, C.A., Rivarola, V.A., Palacios, R.E.
Formato: JOUR
Materias:
apoptosis
brain tumor
colorectal tumor
conjugated polymer nanoparticles
metallated porphyrin
photodynamic therapy
ROS
conjugated polymer nanoparticle
metalloporphyrin
nanoparticle
unclassified drug
Article
biocompatibility
brain tumor cell line
cell damage
cell killing
cell membrane
cellular distribution
colorectal cancer cell line
controlled study
cytotoxicity
drug mechanism
human
human cell
in vitro study
lysosome
macrophage
oxidative stress
photochemistry
priority journal
RAW 264.7 cell line
SW480 cell line
T98G cell line
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_17435889_v13_n6_p605_Ibarra
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_17435889_v13_n6_p605_Ibarra
record_format dspace
spelling todo:paper_17435889_v13_n6_p605_Ibarra2023-10-03T16:31:23Z Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells Ibarra, L.E. Porcal, G.V. Macor, L.P. Ponzio, R.A. Spada, R.M. Lorente, C. Chesta, C.A. Rivarola, V.A. Palacios, R.E. apoptosis brain tumor colorectal tumor conjugated polymer nanoparticles metallated porphyrin photodynamic therapy ROS conjugated polymer nanoparticle metalloporphyrin nanoparticle unclassified drug apoptosis Article biocompatibility brain tumor cell line cell damage cell killing cell membrane cellular distribution colorectal cancer cell line controlled study cytotoxicity drug mechanism human human cell in vitro study lysosome macrophage oxidative stress photochemistry photodynamic therapy priority journal RAW 264.7 cell line SW480 cell line T98G cell line Aim: Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages. Materials & methods: CPNs were developed employing 9,9-dioctylfluorene-alt-benzothiadiazole, an amphiphilic polymer (PS-PEG-COOH), and platinum octaethylporphyrin. T98G, SW480 and RAW 264.7 cell lines were exposed to CPNs to assess uptake and intracellular localization. Additionally, a PDT protocol using CPNs was employed for the in vitro killing of cancer and macrophage cell lines. Results & conclusion: CPNs were well incorporated into glioblastoma and macrophage cells with localization in lysosomes. SW480 cells were less efficient incorporating CPNs with localization in the plasma membrane. In all cell lines PDT treatment was efficient inducing oxidative stress that triggered apoptosis. © 2018 Future Medicine Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_17435889_v13_n6_p605_Ibarra
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic apoptosis
brain tumor
colorectal tumor
conjugated polymer nanoparticles
metallated porphyrin
photodynamic therapy
ROS
conjugated polymer nanoparticle
metalloporphyrin
nanoparticle
unclassified drug
apoptosis
Article
biocompatibility
brain tumor cell line
cell damage
cell killing
cell membrane
cellular distribution
colorectal cancer cell line
controlled study
cytotoxicity
drug mechanism
human
human cell
in vitro study
lysosome
macrophage
oxidative stress
photochemistry
photodynamic therapy
priority journal
RAW 264.7 cell line
SW480 cell line
T98G cell line
spellingShingle apoptosis
brain tumor
colorectal tumor
conjugated polymer nanoparticles
metallated porphyrin
photodynamic therapy
ROS
conjugated polymer nanoparticle
metalloporphyrin
nanoparticle
unclassified drug
apoptosis
Article
biocompatibility
brain tumor cell line
cell damage
cell killing
cell membrane
cellular distribution
colorectal cancer cell line
controlled study
cytotoxicity
drug mechanism
human
human cell
in vitro study
lysosome
macrophage
oxidative stress
photochemistry
photodynamic therapy
priority journal
RAW 264.7 cell line
SW480 cell line
T98G cell line
Ibarra, L.E.
Porcal, G.V.
Macor, L.P.
Ponzio, R.A.
Spada, R.M.
Lorente, C.
Chesta, C.A.
Rivarola, V.A.
Palacios, R.E.
Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells
topic_facet apoptosis
brain tumor
colorectal tumor
conjugated polymer nanoparticles
metallated porphyrin
photodynamic therapy
ROS
conjugated polymer nanoparticle
metalloporphyrin
nanoparticle
unclassified drug
apoptosis
Article
biocompatibility
brain tumor cell line
cell damage
cell killing
cell membrane
cellular distribution
colorectal cancer cell line
controlled study
cytotoxicity
drug mechanism
human
human cell
in vitro study
lysosome
macrophage
oxidative stress
photochemistry
photodynamic therapy
priority journal
RAW 264.7 cell line
SW480 cell line
T98G cell line
description Aim: Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages. Materials & methods: CPNs were developed employing 9,9-dioctylfluorene-alt-benzothiadiazole, an amphiphilic polymer (PS-PEG-COOH), and platinum octaethylporphyrin. T98G, SW480 and RAW 264.7 cell lines were exposed to CPNs to assess uptake and intracellular localization. Additionally, a PDT protocol using CPNs was employed for the in vitro killing of cancer and macrophage cell lines. Results & conclusion: CPNs were well incorporated into glioblastoma and macrophage cells with localization in lysosomes. SW480 cells were less efficient incorporating CPNs with localization in the plasma membrane. In all cell lines PDT treatment was efficient inducing oxidative stress that triggered apoptosis. © 2018 Future Medicine Ltd.
format JOUR
author Ibarra, L.E.
Porcal, G.V.
Macor, L.P.
Ponzio, R.A.
Spada, R.M.
Lorente, C.
Chesta, C.A.
Rivarola, V.A.
Palacios, R.E.
author_facet Ibarra, L.E.
Porcal, G.V.
Macor, L.P.
Ponzio, R.A.
Spada, R.M.
Lorente, C.
Chesta, C.A.
Rivarola, V.A.
Palacios, R.E.
author_sort Ibarra, L.E.
title Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells
title_short Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells
title_full Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells
title_fullStr Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells
title_full_unstemmed Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells
title_sort metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells
url http://hdl.handle.net/20.500.12110/paper_17435889_v13_n6_p605_Ibarra
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