Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells

Aim: Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages. Materials & methods: CPNs were developed employing 9,9-dioctylfluorene-alt-benzothiadiazo...

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Detalles Bibliográficos
Autores principales: Ibarra, L.E., Porcal, G.V., Macor, L.P., Ponzio, R.A., Spada, R.M., Lorente, C., Chesta, C.A., Rivarola, V.A., Palacios, R.E.
Formato: JOUR
Materias:
apoptosis
brain tumor
colorectal tumor
conjugated polymer nanoparticles
metallated porphyrin
photodynamic therapy
ROS
conjugated polymer nanoparticle
metalloporphyrin
nanoparticle
unclassified drug
Article
biocompatibility
brain tumor cell line
cell damage
cell killing
cell membrane
cellular distribution
colorectal cancer cell line
controlled study
cytotoxicity
drug mechanism
human
human cell
in vitro study
lysosome
macrophage
oxidative stress
photochemistry
priority journal
RAW 264.7 cell line
SW480 cell line
T98G cell line
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_17435889_v13_n6_p605_Ibarra
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Aim: Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages. Materials & methods: CPNs were developed employing 9,9-dioctylfluorene-alt-benzothiadiazole, an amphiphilic polymer (PS-PEG-COOH), and platinum octaethylporphyrin. T98G, SW480 and RAW 264.7 cell lines were exposed to CPNs to assess uptake and intracellular localization. Additionally, a PDT protocol using CPNs was employed for the in vitro killing of cancer and macrophage cell lines. Results & conclusion: CPNs were well incorporated into glioblastoma and macrophage cells with localization in lysosomes. SW480 cells were less efficient incorporating CPNs with localization in the plasma membrane. In all cell lines PDT treatment was efficient inducing oxidative stress that triggered apoptosis. © 2018 Future Medicine Ltd.

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