Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture

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Establishment of arbuscular mycorrhizal (AM) germplasm collections is complex because of the obligate biotrophic nature of AM fungi. Only a few AM species are routinely maintained in monoxenic culture with Ri T-DNA transformed roots as host. Incorporation of new AM species into this culture system i...

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Autores principales:	Bidondo, L.F., Pergola, M., Silvani, V., Colombo, R., Bompadre, J., Godeas, A.
Formato:	JOUR
Materias:	Five successive generations of axenic spores Gigaspora decipiens fungal DNA arbuscular mycorrhiza dicotyledon fungus germplasm host molecular analysis physiological response physiology propagule root spore article carrot chemistry DNA sequence fungus spore genetics Glomeromycota growth, development and aging isolation and purification methodology microbial viability microbiology molecular genetics mycology nucleotide sequence plant root Daucus carota DNA, Fungal Microbial Viability Molecular Sequence Data Mycology Plant Roots Sequence Analysis, DNA Spores, Fungal Arbuscular Bacteria (microorganisms) Fungi
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_18786146_v116_n6_p729_Bidondo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_18786146_v116_n6_p729_Bidondo
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spelling	todo:paper_18786146_v116_n6_p729_Bidondo2023-10-03T16:34:21Z Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture Bidondo, L.F. Pergola, M. Silvani, V. Colombo, R. Bompadre, J. Godeas, A. Five successive generations of axenic spores Gigaspora decipiens fungal DNA arbuscular mycorrhiza dicotyledon fungus germplasm host molecular analysis physiological response physiology propagule root spore article carrot chemistry DNA sequence fungus spore genetics Glomeromycota growth, development and aging isolation and purification methodology microbial viability microbiology molecular genetics mycology nucleotide sequence plant root Daucus carota DNA, Fungal Glomeromycota Microbial Viability Molecular Sequence Data Mycology Plant Roots Sequence Analysis, DNA Spores, Fungal Arbuscular Bacteria (microorganisms) Daucus carota Fungi Gigaspora decipiens Glomeromycota Establishment of arbuscular mycorrhizal (AM) germplasm collections is complex because of the obligate biotrophic nature of AM fungi. Only a few AM species are routinely maintained in monoxenic culture with Ri T-DNA transformed roots as host. Incorporation of new AM species into this culture system is important for molecular, physiological, and taxonomical studies. Here we report for the first time the successful monoxenic culture of Gigaspora decipiens (JA2 strain) with transformed carrot (Daucus carota) roots. In vitro cultures were established from field-collected spores; sub-culture of newly in vitro formed spores was established over five successive generations for a period of 6 y. Although initial culture of field-collected spores was difficult successive sub-cultures appeared to be adapted to the in vitro growing conditions. The JA2 strain of G. decipiens completed its life cycle while maintaining its morphological characteristics, stability, and propagule viability under the monoxenic conditions over several generations. This stable and homogeneous monoxenic material obtained for G. decipiens is part of the Banco de Glomeromycota In Vitro (BGIV, http://www.bgiv.com.ar), and could facilitate morphological, physiological, and molecular analysis of this AM species. © 2012 British Mycological Society. Fil:Pergola, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Silvani, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bompadre, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_18786146_v116_n6_p729_Bidondo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Five successive generations of axenic spores Gigaspora decipiens fungal DNA arbuscular mycorrhiza dicotyledon fungus germplasm host molecular analysis physiological response physiology propagule root spore article carrot chemistry DNA sequence fungus spore genetics Glomeromycota growth, development and aging isolation and purification methodology microbial viability microbiology molecular genetics mycology nucleotide sequence plant root Daucus carota DNA, Fungal Glomeromycota Microbial Viability Molecular Sequence Data Mycology Plant Roots Sequence Analysis, DNA Spores, Fungal Arbuscular Bacteria (microorganisms) Daucus carota Fungi Gigaspora decipiens Glomeromycota
spellingShingle	Five successive generations of axenic spores Gigaspora decipiens fungal DNA arbuscular mycorrhiza dicotyledon fungus germplasm host molecular analysis physiological response physiology propagule root spore article carrot chemistry DNA sequence fungus spore genetics Glomeromycota growth, development and aging isolation and purification methodology microbial viability microbiology molecular genetics mycology nucleotide sequence plant root Daucus carota DNA, Fungal Glomeromycota Microbial Viability Molecular Sequence Data Mycology Plant Roots Sequence Analysis, DNA Spores, Fungal Arbuscular Bacteria (microorganisms) Daucus carota Fungi Gigaspora decipiens Glomeromycota Bidondo, L.F. Pergola, M. Silvani, V. Colombo, R. Bompadre, J. Godeas, A. Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture
topic_facet	Five successive generations of axenic spores Gigaspora decipiens fungal DNA arbuscular mycorrhiza dicotyledon fungus germplasm host molecular analysis physiological response physiology propagule root spore article carrot chemistry DNA sequence fungus spore genetics Glomeromycota growth, development and aging isolation and purification methodology microbial viability microbiology molecular genetics mycology nucleotide sequence plant root Daucus carota DNA, Fungal Glomeromycota Microbial Viability Molecular Sequence Data Mycology Plant Roots Sequence Analysis, DNA Spores, Fungal Arbuscular Bacteria (microorganisms) Daucus carota Fungi Gigaspora decipiens Glomeromycota
description	Establishment of arbuscular mycorrhizal (AM) germplasm collections is complex because of the obligate biotrophic nature of AM fungi. Only a few AM species are routinely maintained in monoxenic culture with Ri T-DNA transformed roots as host. Incorporation of new AM species into this culture system is important for molecular, physiological, and taxonomical studies. Here we report for the first time the successful monoxenic culture of Gigaspora decipiens (JA2 strain) with transformed carrot (Daucus carota) roots. In vitro cultures were established from field-collected spores; sub-culture of newly in vitro formed spores was established over five successive generations for a period of 6 y. Although initial culture of field-collected spores was difficult successive sub-cultures appeared to be adapted to the in vitro growing conditions. The JA2 strain of G. decipiens completed its life cycle while maintaining its morphological characteristics, stability, and propagule viability under the monoxenic conditions over several generations. This stable and homogeneous monoxenic material obtained for G. decipiens is part of the Banco de Glomeromycota In Vitro (BGIV, http://www.bgiv.com.ar), and could facilitate morphological, physiological, and molecular analysis of this AM species. © 2012 British Mycological Society.
format	JOUR
author	Bidondo, L.F. Pergola, M. Silvani, V. Colombo, R. Bompadre, J. Godeas, A.
author_facet	Bidondo, L.F. Pergola, M. Silvani, V. Colombo, R. Bompadre, J. Godeas, A.
author_sort	Bidondo, L.F.
title	Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture
title_short	Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture
title_full	Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture
title_fullStr	Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture
title_full_unstemmed	Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture
title_sort	continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus gigaspora decipiens in root organ culture
url	http://hdl.handle.net/20.500.12110/paper_18786146_v116_n6_p729_Bidondo
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_version_	1807321755478392832

Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture

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