Novel essential role of ethanol oxidation genes at low temperature revealed by transcriptome analysis in the antarctic bacterium pseudomonas extremaustralis
Temperature is one of the most important factors for bacterial growth and development. Cold environments are widely distributed on earth, and psychrotolerant and psychrophilic microorganisms have developed different adaptation strategies to cope with the stress derived from low temperatures. Pseudom...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_19326203_v10_n12_p_Tribelli |
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todo:paper_19326203_v10_n12_p_Tribelli |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
alcohol dehydrogenase aldehyde dehydrogenase cytochrome transcriptome tricarboxylic acid alcohol alcohol dehydrogenase alcohol oxidation amino acid metabolism Article azu gene bacterial gene bacterial growth bacterial survival cold acclimatization controlled study cyoA gene cyoB gene cyoC gene cyoD gene down regulation enzyme activity exaA gene exaB gene exaC gene fleQ gene flG gene flH gene flhA gene fliF gene fliG gene fliM gene flK gene flL gene gabD gene gabT gene gas chromatography gene function gene identification genetic association low temperature mobA gene mobB gene nonhuman potABCD gene potFGHI gene pqqB gene protein metabolism Pseudomonas Pseudomonas extremaustralis RNA analysis RNA sequence sequence analysis signal transduction strain identification transcription regulation transcriptomics upregulation Antarctica bacterial gene cold gene expression profiling gene expression regulation genetics growth, development and aging metabolism open reading frame oxidation reduction reaction Pseudomonas software Alcohol Dehydrogenase Antarctic Regions Cold Temperature Ethanol Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Bacterial Open Reading Frames Oxidation-Reduction Pseudomonas Software Up-Regulation |
| spellingShingle |
alcohol dehydrogenase aldehyde dehydrogenase cytochrome transcriptome tricarboxylic acid alcohol alcohol dehydrogenase alcohol oxidation amino acid metabolism Article azu gene bacterial gene bacterial growth bacterial survival cold acclimatization controlled study cyoA gene cyoB gene cyoC gene cyoD gene down regulation enzyme activity exaA gene exaB gene exaC gene fleQ gene flG gene flH gene flhA gene fliF gene fliG gene fliM gene flK gene flL gene gabD gene gabT gene gas chromatography gene function gene identification genetic association low temperature mobA gene mobB gene nonhuman potABCD gene potFGHI gene pqqB gene protein metabolism Pseudomonas Pseudomonas extremaustralis RNA analysis RNA sequence sequence analysis signal transduction strain identification transcription regulation transcriptomics upregulation Antarctica bacterial gene cold gene expression profiling gene expression regulation genetics growth, development and aging metabolism open reading frame oxidation reduction reaction Pseudomonas software Alcohol Dehydrogenase Antarctic Regions Cold Temperature Ethanol Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Bacterial Open Reading Frames Oxidation-Reduction Pseudomonas Software Up-Regulation Tribelli, P.M. Venero, E.C.S. Ricardi, M.M. Gómez-Lozano, M. Iustman, L.J.R. Molin, S. López, N.I. Novel essential role of ethanol oxidation genes at low temperature revealed by transcriptome analysis in the antarctic bacterium pseudomonas extremaustralis |
| topic_facet |
alcohol dehydrogenase aldehyde dehydrogenase cytochrome transcriptome tricarboxylic acid alcohol alcohol dehydrogenase alcohol oxidation amino acid metabolism Article azu gene bacterial gene bacterial growth bacterial survival cold acclimatization controlled study cyoA gene cyoB gene cyoC gene cyoD gene down regulation enzyme activity exaA gene exaB gene exaC gene fleQ gene flG gene flH gene flhA gene fliF gene fliG gene fliM gene flK gene flL gene gabD gene gabT gene gas chromatography gene function gene identification genetic association low temperature mobA gene mobB gene nonhuman potABCD gene potFGHI gene pqqB gene protein metabolism Pseudomonas Pseudomonas extremaustralis RNA analysis RNA sequence sequence analysis signal transduction strain identification transcription regulation transcriptomics upregulation Antarctica bacterial gene cold gene expression profiling gene expression regulation genetics growth, development and aging metabolism open reading frame oxidation reduction reaction Pseudomonas software Alcohol Dehydrogenase Antarctic Regions Cold Temperature Ethanol Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Bacterial Open Reading Frames Oxidation-Reduction Pseudomonas Software Up-Regulation |
| description |
Temperature is one of the most important factors for bacterial growth and development. Cold environments are widely distributed on earth, and psychrotolerant and psychrophilic microorganisms have developed different adaptation strategies to cope with the stress derived from low temperatures. Pseudomonas extremaustralis is an Antarctic bacterium able to grow under low temperatures and to produce high amounts of polyhydroxyalkanoates (PHAs). In this work, we analyzed the genome-wide transcriptome by RNA deepsequencing technology of early exponential cultures of P. extremaustralis growing in LB (Luria Broth) supplemented with sodium octanoate to favor PHA accumulation at 8°C and 30°C. We found that genes involved in primary metabolism, including tricarboxylic acid cycle (TCA) related genes, as well as cytochromes and amino acid metabolism coding genes, were repressed at low temperature. Among up-regulated genes, those coding for transcriptional regulatory and signal transduction proteins were over-represented at cold conditions. Remarkably, we found that genes involved in ethanol oxidation, exaA, exaB and exaC, encoding a pyrroloquinoline quinone (PQQ)-dependent ethanol dehydrogenase, the cytochrome c550 and an aldehyde dehydrogenase respectively, were up-regulated. Along with RNA-seq experiments, analysis of mutant strains for pqqB (PQQ biosynthesis protein B) and exaA were carried out. We found that the exaA and pqqB genes are essential for growth under low temperature in LB supplemented with sodium octanoate. Additionally, prosaniline assay measurements showed the presence of alcohol dehydrogenase activity at both 8°C and 30°C, while the activity was abolished in a pqqB mutant strain. These results together with the detection of ethanol by gas chromatography in P. extremaustralis cultures grown at 8°C support the conclusion that this pathway is important under cold conditions. The obtained results have led to the identification of novel components involved in cold adaptation mechanisms in this bacterium, suggesting for the first time a role of the ethanol oxidation pathway for bacterial growth at low temperatures. © 2015 Tribelli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| format |
JOUR |
| author |
Tribelli, P.M. Venero, E.C.S. Ricardi, M.M. Gómez-Lozano, M. Iustman, L.J.R. Molin, S. López, N.I. |
| author_facet |
Tribelli, P.M. Venero, E.C.S. Ricardi, M.M. Gómez-Lozano, M. Iustman, L.J.R. Molin, S. López, N.I. |
| author_sort |
Tribelli, P.M. |
| title |
Novel essential role of ethanol oxidation genes at low temperature revealed by transcriptome analysis in the antarctic bacterium pseudomonas extremaustralis |
| title_short |
Novel essential role of ethanol oxidation genes at low temperature revealed by transcriptome analysis in the antarctic bacterium pseudomonas extremaustralis |
| title_full |
Novel essential role of ethanol oxidation genes at low temperature revealed by transcriptome analysis in the antarctic bacterium pseudomonas extremaustralis |
| title_fullStr |
Novel essential role of ethanol oxidation genes at low temperature revealed by transcriptome analysis in the antarctic bacterium pseudomonas extremaustralis |
| title_full_unstemmed |
Novel essential role of ethanol oxidation genes at low temperature revealed by transcriptome analysis in the antarctic bacterium pseudomonas extremaustralis |
| title_sort |
novel essential role of ethanol oxidation genes at low temperature revealed by transcriptome analysis in the antarctic bacterium pseudomonas extremaustralis |
| url |
http://hdl.handle.net/20.500.12110/paper_19326203_v10_n12_p_Tribelli |
| work_keys_str_mv |
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1807320544228409344 |
| spelling |
todo:paper_19326203_v10_n12_p_Tribelli2023-10-03T16:34:34Z Novel essential role of ethanol oxidation genes at low temperature revealed by transcriptome analysis in the antarctic bacterium pseudomonas extremaustralis Tribelli, P.M. Venero, E.C.S. Ricardi, M.M. Gómez-Lozano, M. Iustman, L.J.R. Molin, S. López, N.I. alcohol dehydrogenase aldehyde dehydrogenase cytochrome transcriptome tricarboxylic acid alcohol alcohol dehydrogenase alcohol oxidation amino acid metabolism Article azu gene bacterial gene bacterial growth bacterial survival cold acclimatization controlled study cyoA gene cyoB gene cyoC gene cyoD gene down regulation enzyme activity exaA gene exaB gene exaC gene fleQ gene flG gene flH gene flhA gene fliF gene fliG gene fliM gene flK gene flL gene gabD gene gabT gene gas chromatography gene function gene identification genetic association low temperature mobA gene mobB gene nonhuman potABCD gene potFGHI gene pqqB gene protein metabolism Pseudomonas Pseudomonas extremaustralis RNA analysis RNA sequence sequence analysis signal transduction strain identification transcription regulation transcriptomics upregulation Antarctica bacterial gene cold gene expression profiling gene expression regulation genetics growth, development and aging metabolism open reading frame oxidation reduction reaction Pseudomonas software Alcohol Dehydrogenase Antarctic Regions Cold Temperature Ethanol Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Bacterial Open Reading Frames Oxidation-Reduction Pseudomonas Software Up-Regulation Temperature is one of the most important factors for bacterial growth and development. Cold environments are widely distributed on earth, and psychrotolerant and psychrophilic microorganisms have developed different adaptation strategies to cope with the stress derived from low temperatures. Pseudomonas extremaustralis is an Antarctic bacterium able to grow under low temperatures and to produce high amounts of polyhydroxyalkanoates (PHAs). In this work, we analyzed the genome-wide transcriptome by RNA deepsequencing technology of early exponential cultures of P. extremaustralis growing in LB (Luria Broth) supplemented with sodium octanoate to favor PHA accumulation at 8°C and 30°C. We found that genes involved in primary metabolism, including tricarboxylic acid cycle (TCA) related genes, as well as cytochromes and amino acid metabolism coding genes, were repressed at low temperature. Among up-regulated genes, those coding for transcriptional regulatory and signal transduction proteins were over-represented at cold conditions. Remarkably, we found that genes involved in ethanol oxidation, exaA, exaB and exaC, encoding a pyrroloquinoline quinone (PQQ)-dependent ethanol dehydrogenase, the cytochrome c550 and an aldehyde dehydrogenase respectively, were up-regulated. Along with RNA-seq experiments, analysis of mutant strains for pqqB (PQQ biosynthesis protein B) and exaA were carried out. We found that the exaA and pqqB genes are essential for growth under low temperature in LB supplemented with sodium octanoate. Additionally, prosaniline assay measurements showed the presence of alcohol dehydrogenase activity at both 8°C and 30°C, while the activity was abolished in a pqqB mutant strain. These results together with the detection of ethanol by gas chromatography in P. extremaustralis cultures grown at 8°C support the conclusion that this pathway is important under cold conditions. The obtained results have led to the identification of novel components involved in cold adaptation mechanisms in this bacterium, suggesting for the first time a role of the ethanol oxidation pathway for bacterial growth at low temperatures. © 2015 Tribelli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ricardi, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iustman, L.J.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v10_n12_p_Tribelli |