The TATA-less promoter of VP1, a plant gene controlling seed germination

Vp1 is a seed-specific gene involved in the control of dormancy and germination. We here present the complete sequence of the sorghum vp1 promoter/enhancer region highlighting its main features, especially the lack of canonical TATA and CAAT boxes and the presence of elements responsive to abscisic...

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Autores principales: Carrari, F., Frankel, N., Lijavetzky, D., Benech-Arnold, R., Sánchez, R., Iusem, N.D.
Formato: JOUR
Materias:
germination
MITE
Sorghum bicolor
TATA-less promoter
vpl gene
Base Sequence
Cereals
Germination
Molecular Sequence Data
Plant Proteins
Promoter Regions (Genetics)
TATA Box
Trans-Activators
Transcription Initiation Site
Acari
Arabidopsis
Embryophyta
Zea mays
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19401736_v12_n2_p107_Carrari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_19401736_v12_n2_p107_Carrari
record_format dspace
spelling todo:paper_19401736_v12_n2_p107_Carrari2023-10-03T16:36:52Z The TATA-less promoter of VP1, a plant gene controlling seed germination Carrari, F. Frankel, N. Lijavetzky, D. Benech-Arnold, R. Sánchez, R. Iusem, N.D. germination MITE Sorghum bicolor TATA-less promoter vpl gene Base Sequence Cereals Germination Molecular Sequence Data Plant Proteins Promoter Regions (Genetics) TATA Box Trans-Activators Transcription Initiation Site Acari Arabidopsis Embryophyta Sorghum bicolor Zea mays Vp1 is a seed-specific gene involved in the control of dormancy and germination. We here present the complete sequence of the sorghum vp1 promoter/enhancer region highlighting its main features, especially the lack of canonical TATA and CAAT boxes and the presence of elements responsive to abscisic acid and light. The region closest to the start of transcription is highly homologous to the partial proximal sequence reported for the maize vp1 promoter. This region is interrupted by a 57-nt stretch containing 14 CT microsatellite repeats. We observed a poor overall homology to the promoter from abi3 gene, the Arabidopsis counterpart bearing a similar coding sequence. However, there exists a high degree of homology (89%) between a TATA-rich 103-bp stretch of the sorghum vp1 promoter located about 700 nt upstream of the startpoint and miniature inverted transposable elements (MITEs) interspersed within the sorghum seed-specific kafirin cluster. This sorghum MITE-like element displays considerable homology (68%) to the TATA-less promoter from the sorghum NADP-malate dehydrogenase gene and lesser similarity to the Tourist, Pilgrim and Batuta MITEs previously identified within the promoter from the maize Abp1 (auxin-binding protein) gene. © 2001 OPA (Overseas Publishen Aswciatinn) N V. Fil:Carrari, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Frankel, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lijavetzky, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19401736_v12_n2_p107_Carrari
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic germination
MITE
Sorghum bicolor
TATA-less promoter
vpl gene
Base Sequence
Cereals
Germination
Molecular Sequence Data
Plant Proteins
Promoter Regions (Genetics)
TATA Box
Trans-Activators
Transcription Initiation Site
Acari
Arabidopsis
Embryophyta
Sorghum bicolor
Zea mays
spellingShingle germination
MITE
Sorghum bicolor
TATA-less promoter
vpl gene
Base Sequence
Cereals
Germination
Molecular Sequence Data
Plant Proteins
Promoter Regions (Genetics)
TATA Box
Trans-Activators
Transcription Initiation Site
Acari
Arabidopsis
Embryophyta
Sorghum bicolor
Zea mays
Carrari, F.
Frankel, N.
Lijavetzky, D.
Benech-Arnold, R.
Sánchez, R.
Iusem, N.D.
The TATA-less promoter of VP1, a plant gene controlling seed germination
topic_facet germination
MITE
Sorghum bicolor
TATA-less promoter
vpl gene
Base Sequence
Cereals
Germination
Molecular Sequence Data
Plant Proteins
Promoter Regions (Genetics)
TATA Box
Trans-Activators
Transcription Initiation Site
Acari
Arabidopsis
Embryophyta
Sorghum bicolor
Zea mays
description Vp1 is a seed-specific gene involved in the control of dormancy and germination. We here present the complete sequence of the sorghum vp1 promoter/enhancer region highlighting its main features, especially the lack of canonical TATA and CAAT boxes and the presence of elements responsive to abscisic acid and light. The region closest to the start of transcription is highly homologous to the partial proximal sequence reported for the maize vp1 promoter. This region is interrupted by a 57-nt stretch containing 14 CT microsatellite repeats. We observed a poor overall homology to the promoter from abi3 gene, the Arabidopsis counterpart bearing a similar coding sequence. However, there exists a high degree of homology (89%) between a TATA-rich 103-bp stretch of the sorghum vp1 promoter located about 700 nt upstream of the startpoint and miniature inverted transposable elements (MITEs) interspersed within the sorghum seed-specific kafirin cluster. This sorghum MITE-like element displays considerable homology (68%) to the TATA-less promoter from the sorghum NADP-malate dehydrogenase gene and lesser similarity to the Tourist, Pilgrim and Batuta MITEs previously identified within the promoter from the maize Abp1 (auxin-binding protein) gene. © 2001 OPA (Overseas Publishen Aswciatinn) N V.
format JOUR
author Carrari, F.
Frankel, N.
Lijavetzky, D.
Benech-Arnold, R.
Sánchez, R.
Iusem, N.D.
author_facet Carrari, F.
Frankel, N.
Lijavetzky, D.
Benech-Arnold, R.
Sánchez, R.
Iusem, N.D.
author_sort Carrari, F.
title The TATA-less promoter of VP1, a plant gene controlling seed germination
title_short The TATA-less promoter of VP1, a plant gene controlling seed germination
title_full The TATA-less promoter of VP1, a plant gene controlling seed germination
title_fullStr The TATA-less promoter of VP1, a plant gene controlling seed germination
title_full_unstemmed The TATA-less promoter of VP1, a plant gene controlling seed germination
title_sort tata-less promoter of vp1, a plant gene controlling seed germination
url http://hdl.handle.net/20.500.12110/paper_19401736_v12_n2_p107_Carrari
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