Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli
Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia c...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_21647860_v1_n5_p260_Traglia |
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todo:paper_21647860_v1_n5_p260_Traglia2023-10-03T16:39:37Z Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli Traglia, G.M. Sala, C.D. Bass, J.I.F. Soler-Bistué, A.J.C. Zorreguieta, A. Ramírez, M.S. Tolmasky, M.E. Drug discovery Drug resistance Gene therapy Microbiology Nucleic acids alexa fluor 488 locked nucleic acid oligomer unclassified drug antisense therapy article bacterial cell cell culture confocal microscopy conjugation controlled study cytoplasm drug penetration Escherichia coli flow cytometry gene delivery system nonhuman priority journal Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cytoplasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a sta- tionary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm.© Mary Ann Liebert, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_21647860_v1_n5_p260_Traglia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Drug discovery Drug resistance Gene therapy Microbiology Nucleic acids alexa fluor 488 locked nucleic acid oligomer unclassified drug antisense therapy article bacterial cell cell culture confocal microscopy conjugation controlled study cytoplasm drug penetration Escherichia coli flow cytometry gene delivery system nonhuman priority journal |
| spellingShingle |
Drug discovery Drug resistance Gene therapy Microbiology Nucleic acids alexa fluor 488 locked nucleic acid oligomer unclassified drug antisense therapy article bacterial cell cell culture confocal microscopy conjugation controlled study cytoplasm drug penetration Escherichia coli flow cytometry gene delivery system nonhuman priority journal Traglia, G.M. Sala, C.D. Bass, J.I.F. Soler-Bistué, A.J.C. Zorreguieta, A. Ramírez, M.S. Tolmasky, M.E. Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli |
| topic_facet |
Drug discovery Drug resistance Gene therapy Microbiology Nucleic acids alexa fluor 488 locked nucleic acid oligomer unclassified drug antisense therapy article bacterial cell cell culture confocal microscopy conjugation controlled study cytoplasm drug penetration Escherichia coli flow cytometry gene delivery system nonhuman priority journal |
| description |
Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cytoplasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a sta- tionary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm.© Mary Ann Liebert, Inc. |
| format |
JOUR |
| author |
Traglia, G.M. Sala, C.D. Bass, J.I.F. Soler-Bistué, A.J.C. Zorreguieta, A. Ramírez, M.S. Tolmasky, M.E. |
| author_facet |
Traglia, G.M. Sala, C.D. Bass, J.I.F. Soler-Bistué, A.J.C. Zorreguieta, A. Ramírez, M.S. Tolmasky, M.E. |
| author_sort |
Traglia, G.M. |
| title |
Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli |
| title_short |
Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli |
| title_full |
Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli |
| title_fullStr |
Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli |
| title_full_unstemmed |
Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli |
| title_sort |
internalization of locked nucleic acids/dna hybrid oligomers into escherichia coli |
| url |
http://hdl.handle.net/20.500.12110/paper_21647860_v1_n5_p260_Traglia |
| work_keys_str_mv |
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