Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli

Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia c...

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Detalles Bibliográficos
Autores principales: Traglia, G.M., Sala, C.D., Bass, J.I.F., Soler-Bistué, A.J.C., Zorreguieta, A., Ramírez, M.S., Tolmasky, M.E.
Formato: JOUR
Materias:
Drug discovery
Drug resistance
Gene therapy
Microbiology
Nucleic acids
alexa fluor 488
locked nucleic acid
oligomer
unclassified drug
antisense therapy
article
bacterial cell
cell culture
confocal microscopy
conjugation
controlled study
cytoplasm
drug penetration
Escherichia coli
flow cytometry
gene delivery system
nonhuman
priority journal
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_21647860_v1_n5_p260_Traglia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cytoplasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a sta- tionary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm.© Mary Ann Liebert, Inc.

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