Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells
Since antiretroviral therapy suppresses but does not eradicate HIV-1 infection, methods to purge viral reservoirs are required. Many strategies involve the reactivation of chronically HIV infected cells to induce the expression of integrated viral genome. In this study, five bioactive compounds, the...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Barquero |
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todo:paper_23146133_v2014_n_p_Barquero2023-10-03T16:40:56Z Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells Barquero, A.A. Dávola, M.E. Riva, D.A. Mersich, S.E. Alché, L.E. 1 cinnamoyl 3,11 dihydroxymeliacarpin 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antigen p24 antiretrovirus agent curcumin interleukin 2 interleukin 6 interleukin 7 nordihydroguaiaretic acid plant medicinal product reactive oxygen metabolite stigmasterol tumor necrosis factor alpha unclassified drug 1-cinnamoyl-3,11-dihydroxymeliacarpin 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one biological factor cholestane derivative interleukin 6 limonoid plant extract reactive oxygen metabolite stigmasterol tumor necrosis factor alpha article cell viability controlled study cytokine production cytokine release drug mechanism human human cell Human immunodeficiency virus 1 infection lymphocyte monocyte macrophage precursor cell oxidation reduction state protein expression virus reactivation virus replication analogs and derivatives cell line DNA replication drug effects Human immunodeficiency virus 1 Human immunodeficiency virus infection metabolism monocyte tumor cell line U937 cell line virology virus replication Biological Factors Cell Line Cell Line, Tumor Cholestanones Curcumin DNA Replication HIV Infections HIV-1 Humans Interleukin-6 Limonins Masoprocol Monocytes Plant Extracts Reactive Oxygen Species Stigmasterol Tumor Necrosis Factor-alpha U937 Cells Virus Replication Since antiretroviral therapy suppresses but does not eradicate HIV-1 infection, methods to purge viral reservoirs are required. Many strategies involve the reactivation of chronically HIV infected cells to induce the expression of integrated viral genome. In this study, five bioactive compounds, the plant derivatives 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM), nordihydroguaiaretic acid (NDGA), and curcumin (Cur) and the synthetic stigmasterol analogs (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (compound 2), were evaluated for their ability to elicit HIV replication in promonocytic (U1) and lymphocytic (H9+) HIV-1 chronically infected cells. The results revealed that natural compounds CDM, NDGA, and Cur were able to increase HIV-1 p24 antigen, determined by ELISA, only in latently infected promonocytic cells. CDM would reactivate HIV from latency by modulating the release of IL-6 and TNF-α, since the amount of both cytokines measured through ELISA significantly increased in U1 treated cells. Besides, NDGA increased ROS production, which might be related to the increase on p24 level observed in NDGA treated U1. These findings suggest that CDM, NDGA, and Cur might be candidates for further studies on latency-reversing therapeutics to eliminate latently HIV-1 reservoirs. © 2014 Andrea Alejandra Barquero et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Barquero |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
1 cinnamoyl 3,11 dihydroxymeliacarpin 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antigen p24 antiretrovirus agent curcumin interleukin 2 interleukin 6 interleukin 7 nordihydroguaiaretic acid plant medicinal product reactive oxygen metabolite stigmasterol tumor necrosis factor alpha unclassified drug 1-cinnamoyl-3,11-dihydroxymeliacarpin 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one biological factor cholestane derivative interleukin 6 limonoid plant extract reactive oxygen metabolite stigmasterol tumor necrosis factor alpha article cell viability controlled study cytokine production cytokine release drug mechanism human human cell Human immunodeficiency virus 1 infection lymphocyte monocyte macrophage precursor cell oxidation reduction state protein expression virus reactivation virus replication analogs and derivatives cell line DNA replication drug effects Human immunodeficiency virus 1 Human immunodeficiency virus infection metabolism monocyte tumor cell line U937 cell line virology virus replication Biological Factors Cell Line Cell Line, Tumor Cholestanones Curcumin DNA Replication HIV Infections HIV-1 Humans Interleukin-6 Limonins Masoprocol Monocytes Plant Extracts Reactive Oxygen Species Stigmasterol Tumor Necrosis Factor-alpha U937 Cells Virus Replication |
| spellingShingle |
1 cinnamoyl 3,11 dihydroxymeliacarpin 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antigen p24 antiretrovirus agent curcumin interleukin 2 interleukin 6 interleukin 7 nordihydroguaiaretic acid plant medicinal product reactive oxygen metabolite stigmasterol tumor necrosis factor alpha unclassified drug 1-cinnamoyl-3,11-dihydroxymeliacarpin 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one biological factor cholestane derivative interleukin 6 limonoid plant extract reactive oxygen metabolite stigmasterol tumor necrosis factor alpha article cell viability controlled study cytokine production cytokine release drug mechanism human human cell Human immunodeficiency virus 1 infection lymphocyte monocyte macrophage precursor cell oxidation reduction state protein expression virus reactivation virus replication analogs and derivatives cell line DNA replication drug effects Human immunodeficiency virus 1 Human immunodeficiency virus infection metabolism monocyte tumor cell line U937 cell line virology virus replication Biological Factors Cell Line Cell Line, Tumor Cholestanones Curcumin DNA Replication HIV Infections HIV-1 Humans Interleukin-6 Limonins Masoprocol Monocytes Plant Extracts Reactive Oxygen Species Stigmasterol Tumor Necrosis Factor-alpha U937 Cells Virus Replication Barquero, A.A. Dávola, M.E. Riva, D.A. Mersich, S.E. Alché, L.E. Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells |
| topic_facet |
1 cinnamoyl 3,11 dihydroxymeliacarpin 22,23 dihydroxystigmast 4 en 3 one 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one antigen p24 antiretrovirus agent curcumin interleukin 2 interleukin 6 interleukin 7 nordihydroguaiaretic acid plant medicinal product reactive oxygen metabolite stigmasterol tumor necrosis factor alpha unclassified drug 1-cinnamoyl-3,11-dihydroxymeliacarpin 22,23-dihydroxystigmast-4-en-3-one 3-bromo-5,22,23-trihydroxystigmastan-6-one biological factor cholestane derivative interleukin 6 limonoid plant extract reactive oxygen metabolite stigmasterol tumor necrosis factor alpha article cell viability controlled study cytokine production cytokine release drug mechanism human human cell Human immunodeficiency virus 1 infection lymphocyte monocyte macrophage precursor cell oxidation reduction state protein expression virus reactivation virus replication analogs and derivatives cell line DNA replication drug effects Human immunodeficiency virus 1 Human immunodeficiency virus infection metabolism monocyte tumor cell line U937 cell line virology virus replication Biological Factors Cell Line Cell Line, Tumor Cholestanones Curcumin DNA Replication HIV Infections HIV-1 Humans Interleukin-6 Limonins Masoprocol Monocytes Plant Extracts Reactive Oxygen Species Stigmasterol Tumor Necrosis Factor-alpha U937 Cells Virus Replication |
| description |
Since antiretroviral therapy suppresses but does not eradicate HIV-1 infection, methods to purge viral reservoirs are required. Many strategies involve the reactivation of chronically HIV infected cells to induce the expression of integrated viral genome. In this study, five bioactive compounds, the plant derivatives 1-cinnamoyl-3,11-dihydroxymeliacarpin (CDM), nordihydroguaiaretic acid (NDGA), and curcumin (Cur) and the synthetic stigmasterol analogs (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (compound 2), were evaluated for their ability to elicit HIV replication in promonocytic (U1) and lymphocytic (H9+) HIV-1 chronically infected cells. The results revealed that natural compounds CDM, NDGA, and Cur were able to increase HIV-1 p24 antigen, determined by ELISA, only in latently infected promonocytic cells. CDM would reactivate HIV from latency by modulating the release of IL-6 and TNF-α, since the amount of both cytokines measured through ELISA significantly increased in U1 treated cells. Besides, NDGA increased ROS production, which might be related to the increase on p24 level observed in NDGA treated U1. These findings suggest that CDM, NDGA, and Cur might be candidates for further studies on latency-reversing therapeutics to eliminate latently HIV-1 reservoirs. © 2014 Andrea Alejandra Barquero et al. |
| format |
JOUR |
| author |
Barquero, A.A. Dávola, M.E. Riva, D.A. Mersich, S.E. Alché, L.E. |
| author_facet |
Barquero, A.A. Dávola, M.E. Riva, D.A. Mersich, S.E. Alché, L.E. |
| author_sort |
Barquero, A.A. |
| title |
Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells |
| title_short |
Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells |
| title_full |
Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells |
| title_fullStr |
Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells |
| title_full_unstemmed |
Naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells |
| title_sort |
naturally occurring compounds elicit hiv-1 replication in chronically infected promonocytic cells |
| url |
http://hdl.handle.net/20.500.12110/paper_23146133_v2014_n_p_Barquero |
| work_keys_str_mv |
AT barqueroaa naturallyoccurringcompoundselicithiv1replicationinchronicallyinfectedpromonocyticcells AT davolame naturallyoccurringcompoundselicithiv1replicationinchronicallyinfectedpromonocyticcells AT rivada naturallyoccurringcompoundselicithiv1replicationinchronicallyinfectedpromonocyticcells AT mersichse naturallyoccurringcompoundselicithiv1replicationinchronicallyinfectedpromonocyticcells AT alchele naturallyoccurringcompoundselicithiv1replicationinchronicallyinfectedpromonocyticcells |
| _version_ |
1807324317212475392 |