Electrochemical impedance biosensor for Chagas Disease diagnosis in clinical samples

Chagas disease (CD) is one of the main neglected tropical diseases, diagnosed mainly by serological tests performed in centralized laboratories, which severely limits the clinical management of the disease in communities with scarce resources. Herein, an electrochemical impedance biosensor for the d...

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Autores principales: Cisneros, José Sebastián, Chain, Cecilia Yamil, Daza Millone, María Antonieta, Labriola, C. A., Scollo, K., Ruiz, A. M., Estrela, P., Vela, María Elena
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Ciencias Exactas
Biología
immunosensor
impedimetric sensor
Chagas disease
cruzipain
self-assembled monolayers
clinical samples
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/156722
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-156722
record_format dspace
spelling I19-R120-10915-1567222023-08-24T04:06:20Z http://sedici.unlp.edu.ar/handle/10915/156722 Electrochemical impedance biosensor for Chagas Disease diagnosis in clinical samples Cisneros, José Sebastián Chain, Cecilia Yamil Daza Millone, María Antonieta Labriola, C. A. Scollo, K. Ruiz, A. M. Estrela, P. Vela, María Elena 2022-12 2023-08-23T14:48:31Z en Ciencias Exactas Biología immunosensor impedimetric sensor Chagas disease cruzipain self-assembled monolayers clinical samples Chagas disease (CD) is one of the main neglected tropical diseases, diagnosed mainly by serological tests performed in centralized laboratories, which severely limits the clinical management of the disease in communities with scarce resources. Herein, an electrochemical impedance biosensor for the detection of CD was developed for the first time using a cruzipain-based sensor surface. The protein, highly immunogenic and isolated from Trypanosoma cruzi, was immobilized over the surface of gold disc electrodes modified with 11-mercaptoundecanoic (MUA) and 6-mercapto-1-hexanol (MCH) self-assembled monolayers (SAMs). Reproducible sensor surfaces, yielding 38 ± 3% coverage as measured by Surface Plasmon Resonance, were obtained by amide coupling of 120 μg/mL cruzipain onto 1/10 MUA/MCH SAMs for 30 min. Under optimized operational conditions, the impedimetric immunosensor recognized specific interactions for anti-T. cruzi antibodies in 1/800 diluted human serum patient samples. The charge transfer resistance of the biosensors increases by ~18% in the presence of the positive samples, whereas the negative samples give rise to a negligible increase of around 6%. An excellent selectivity to clinical samples from patients infected with T. cruzi was obtained. The clear signal difference obtained for positive and negative clinical samples highlights the applicability of the sensors for the point-of-care diagnosis of CD. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
immunosensor
impedimetric sensor
Chagas disease
cruzipain
self-assembled monolayers
clinical samples
spellingShingle Ciencias Exactas
Biología
immunosensor
impedimetric sensor
Chagas disease
cruzipain
self-assembled monolayers
clinical samples
Cisneros, José Sebastián
Chain, Cecilia Yamil
Daza Millone, María Antonieta
Labriola, C. A.
Scollo, K.
Ruiz, A. M.
Estrela, P.
Vela, María Elena
Electrochemical impedance biosensor for Chagas Disease diagnosis in clinical samples
topic_facet Ciencias Exactas
Biología
immunosensor
impedimetric sensor
Chagas disease
cruzipain
self-assembled monolayers
clinical samples
description Chagas disease (CD) is one of the main neglected tropical diseases, diagnosed mainly by serological tests performed in centralized laboratories, which severely limits the clinical management of the disease in communities with scarce resources. Herein, an electrochemical impedance biosensor for the detection of CD was developed for the first time using a cruzipain-based sensor surface. The protein, highly immunogenic and isolated from Trypanosoma cruzi, was immobilized over the surface of gold disc electrodes modified with 11-mercaptoundecanoic (MUA) and 6-mercapto-1-hexanol (MCH) self-assembled monolayers (SAMs). Reproducible sensor surfaces, yielding 38 ± 3% coverage as measured by Surface Plasmon Resonance, were obtained by amide coupling of 120 μg/mL cruzipain onto 1/10 MUA/MCH SAMs for 30 min. Under optimized operational conditions, the impedimetric immunosensor recognized specific interactions for anti-T. cruzi antibodies in 1/800 diluted human serum patient samples. The charge transfer resistance of the biosensors increases by ~18% in the presence of the positive samples, whereas the negative samples give rise to a negligible increase of around 6%. An excellent selectivity to clinical samples from patients infected with T. cruzi was obtained. The clear signal difference obtained for positive and negative clinical samples highlights the applicability of the sensors for the point-of-care diagnosis of CD.
format Articulo
Articulo
author Cisneros, José Sebastián
Chain, Cecilia Yamil
Daza Millone, María Antonieta
Labriola, C. A.
Scollo, K.
Ruiz, A. M.
Estrela, P.
Vela, María Elena
author_facet Cisneros, José Sebastián
Chain, Cecilia Yamil
Daza Millone, María Antonieta
Labriola, C. A.
Scollo, K.
Ruiz, A. M.
Estrela, P.
Vela, María Elena
author_sort Cisneros, José Sebastián
title Electrochemical impedance biosensor for Chagas Disease diagnosis in clinical samples
title_short Electrochemical impedance biosensor for Chagas Disease diagnosis in clinical samples
title_full Electrochemical impedance biosensor for Chagas Disease diagnosis in clinical samples
title_fullStr Electrochemical impedance biosensor for Chagas Disease diagnosis in clinical samples
title_full_unstemmed Electrochemical impedance biosensor for Chagas Disease diagnosis in clinical samples
title_sort electrochemical impedance biosensor for chagas disease diagnosis in clinical samples
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/156722
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AT scollok electrochemicalimpedancebiosensorforchagasdiseasediagnosisinclinicalsamples
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